Cancer, human colon

Human Lung Carcinoma Cell Line Human Cervical Carcinoma Human T-Cell Leukaemia Human Non Small Cell Lung Cancer Laryngeal Cancer Human Colon Cancer Human Colon Cancer... 

Folate antagonists (eg, methotrexate and certain antiepileptics) are used ia treatment for various diseases, but their adininistration can lead to a functional folate deficiency. Folate utilization can be impaired by a depletion of ziac (see Zinc compounds). In humans, the intestinal bmsh border folate conjugase is a ziac metaHoenzyme (72). One study iadicates that the substantial consumption of alcohol, when combiaed with an iaadequate iatake of folate and methionine, may iacrease the risk of colon cancer (73). Based on this study, it is recommended to avoid excess alcohol consumption and iacrease folate iatake to lower the risk of colon cancer. 

Faulty mismatch repair has been finked to fieredi-tary nonpolyposis colon cancer (HNPCC), one of die most common inherited cancers. Genetic smdies finked HNPCC in some families to a region of cfiromosome 2. The gene located, designated hMSH2, was sub-sequendy shown to encode the human analog of the... 

TULLiEZ J and terce f (2000) Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells . Cancer Res, 60 1426-33. 

AVIVI-GREEN c, PLOAK-CHARCON s, MADAR z, SCHWARTZ B (2002) Different molecule events account for butyrate-induced apoptosis in tiw human colon cancer cell line. J. Nutr. 132 1812-18. 

GAMET L, DAViAUD D, DENis-POUXViEL 0, REMESY 0, MURAT J 0 (1992) Effects of shoit-chain fatty acids on growth and differentiation of the human colon-cancer cell line HT29. Int J Cancer. 52 286-9. 

HUDSON A, DiNH p A, KOKUBUN T, siMMONDS M s, GESCHER A (2000) Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. Cancer Epidemiol Biomarkers Prev, 9 (11) 1163-70. 

Carter, O., Bailey, G.S., and Dashwood, R.H., The dietary phytochemical chlorophyllin alters E-cadherin and P-catenin expression in human colon cancer cells International Research Conference on Food, Nutrition and Cancer, J. Nutr., 134,3441S, 2004. 

Kang, S.Y. et al., Tart cherry anthocyanins inhibit tumor development in Apc(Min) mice and reduce prohferation of human colon cancer cells. Cancer Lett., 194, 13, 2003. 

Extraction procedures must be adjusted when separated anthocyanins will be tested in biological studies. We have found that the types of acids used for anthocyanin extraction as well as their residual concentrations in the final extract may affect the results obtained from biological tests. The growth inhibitory effect of anthocyanins on HT29 (human colonic cancer) cells may be overestimated if the residual acid in the extract exerts a toxic effect on the cells. Acetic acid residues in anthocyanin extracts showed less toxicity to HT29 cells than hydrochloric acid when samples were prepared under the same extraction procedure and subjected to the same tests on HT29 cells. In addition, the procedure to remove acids affected the acid residual concentration as well in final anthocyanin extracts, with lyophilization being more successful than rotary evaporation. 

Fiorelli, G. Picariello, L. Martineti, V. Tonelli, F. Brandi, M. L. Estrogen synthesis in human colon cancer epithelial cells. J. Steroid Biochem. Mol. Biol. 1999, 71, 223-230. 

Recently, fucoxanthin has been shown to inhibit the proliferation of human colon cancer cells by a mechanism involving an up-regulation of p21WAFl/Cipl (Das et al., 2005). 

Terasaki, M., Asai, A., Zhang, H. and Nagao, A. 2007. A highly polar xanthophyll of 9 -cis-neoxanthin induces apoptosis in HCT116 human colon cancer cells through mitochondrial dysfunction. Mol Cell Biochem 300 227-237. 

Belley, A., Keller, K., Grove, J. and Chadee, K. (1996) Interaction of LS174T human colon cancer cell mucins with Entamoeba histolytica an in vitro model for colonic disease. Gastroenterology 111, 1484—1492. 

Another human colonic cancer cell line is T84 this develops monolayers of high TER ( 1000 Q cm2) when grown on permeable supports, but cells are not well differentiated and have been described as resembling a colonic crypt cell phenotype. Hence, these cells have been used mainly in studies of epithelial ion transport and are generally not considered to be adequate for drug transport studies, particularly with respect to carrier-mediated processes [10, 79, 82-84]. 

In a somewhat similar scheme, Noguchi et al. (1992) prepared a carboxylate spacer arm by reacting 6-bromohexanoic acid with a dextran polymer. The carboxylate then was aminated with ethylene diamine to form an amine-terminal spacer (Figure 25.15). This dextran derivative finally was reacted with N-Succinimidyl 3-(2-pyridyldithio)propionate (SPDP) (Chapter 5, Section 1.1) to create the desired sulfhydryl-reactive polymer (Section 2.4, this chapter). The SPDP-activated polymer then could be used to prepare an immunoconjugate composed of an antibody against human colon cancer conjugated with the drug mitomycin-C. 

Noguchi, A., Takahashi, T., Yamaguchi, T., Kitamura, K., Takakura, Y., Hashida, M., and Sezaki, H. (1992) Preparation and properties of the immunoconjugate composed of anti-human colon cancer monoclonal antibody and mitomycin C—Dextran conjugate. Bioconjugate Chem. 3, 132-137. 

D. Pirkebner, M. Fuetschi, W. Wittmann, H. Weiss, T. Haller, H. Schramer, R. Margereiter, and A. Amberger, Reduction of intracellular pH inhibits constitutive expression of cyclooxygenase-2 in human colon cancer cells. J. Cell. Physiol. 198, 295-301 (2004). 

Human carcinoembryonic antigen Mouse/human chimeric IgGl antibody (cT84.66) Antibody - mediated cancer therapy (colon cancer, breast cancer and tumor with epithelial origin) N. tabacum cv petit Havana SRI (Transient expression) 35S MSP + 1 mg/kg FLW 42... 

Tang FY, Cho HJ, Pai MH and Chen YH. 2008. Concomitant supplementation of lycopene and eicosapen-taenoic acid inhibits the proliferation of human colon cancer cells. J Nutr Biochem. In press. 

T. J. Yeatman, Activation of c-Src by receptor tyrosine kinases in human colon cancer cells with high metastatic potential, Oncogene 15 3083 (1997). 

In conclusion, phytic acid forms soluble complexes with Ca2+ at intestinal pH under a variety of conditions and fails to inhibit Ca2 bioavailability to mice in our experimental system. Despite the hazard in direct extrapolation of results obtained with animals kept on a well-defined dietary regimen to humans consuming a complex diet, many elements of which affect Ca2+ bioavailability, our data demonstrate the need for a reevaluation of the putative antinutritional properties of dietary phytate. Our further contention that adequate levels of dietary phytate may actually be beneficial due to its food preserving properties and its protection against colonic cancer will warrant a prospective epidemiological human study designed to assess the longterm effects of dietary phytate on mineral bioavailability and inflammatory bowel diseases. 

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