Cell Transformation systems

Sanchez, J.H., Abemethy, D.J. Boreiko, C.J. (1987) Lack of di-(2-ethylhexyl) phthalate activity in the C3H/10T1/2 cell transformation system. Toxicol. In Vitro, 1, 49-53 Sarmer, T. Rivedal, E. (1985) Tests with the Syrian hamster embryo (SHE) cell transformation assay. Prog. Mu tat. Res., 5, 665-671 Sasakawa, S. Mitomi, Y. (1978) Di-2-ethylhexyl phthalate (DEHP) content of blood or blood components stored in plastic bags. Vox Sang., 34, 81-86 Saxena, D.K., Srivastava, S.P, Chandra, S.V. Seth, P.K. (1985) Testicrrlar effects of di-(2-ethylhexyl) phthalate (DEHP) histochemical and histopathological alteratiorrs. Ind. Health, 23, 191-198... 

Abernethy, D.J., Frazelle, J.H. Boreiko, C.J. (1982) Effects of ethanol, acetaldehyde and acetic acid in the C3H/I0TI/2 cl8 cell transformation system. Environ. Mol. Mutagen., 4. 331 American Conference of Governmental Industrial Hygienists (1991) Documentation of the Thres-... 

Sanchez JH, Abemethy DJ, Boreiko CJ. 1987. Lack of di-(2-ethylhexyl) phthalate activity in the C3H/10T1/2 cell transformation system Toxicol in Vitro l(l) 49-53. 

In this chapter, emphasis will be placed on the use of early passage Syrian hamster cells for identifying carcinogens. Results from this cell transformation system will be correlated with reported results from the bacterial mutagenesis system and chronic carcinogenicity tests in animals. 

Mammalian Cell Transformation Systems as Prescreens for Identifying Carcinogens in the Environment... 

Most in vitro cell transformation systems employ fibroblast cells that produce sarcomas when inoculated into suitable test animals. Since most human cancers are carcinomas of epithelial cell origin, it would seem more relevant to use cultured epithelial cells. However, only relatively few studies... 

TABLE 1. Mammalian Cell Transformation Systems Employing Fibroblast... 

Most of the fibroblast cell transformation systems employ cells derived from the fetal tissues of various rodents such as hamster, rat, mouse, and guinea pig. The transformation of human cells has been reported only recently. Use of these or similar human cell lines in bioassay test systems must await further development. 

The fungal transformation of estrogens, by enzymes or cells, seems to produce metabolites with reduced estrogenic activity in short periods, usually days or even few hours. However, only oligomeric products have been identified in enzymatic processes and a lack in the identification of metabolites derived from whole cell fungal systems is still observed. 

In order to reduce the time required to confirm the accumulation of a given recombinant protein, we have developed a cell culture system in which transgenic alfalfa callus material produced at the proliferation step of Agrobacterium-based transformation is used to initiate cell cultures. These cell suspensions can be subcultured to sustain batch production of modest protein amounts. The protein blot shown in Fig. 1.2 demonstrates our ability to detect a recombinant protein in total... 

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