BALB/c 3T3 cell transformation assay

Fig. 5 BALB/c 3T3 cell transformation assay example of focus induced in BALB/c 3T3 clone A31-1-1 after exposure to a carcinogenic compound. Cells are stained with Giemsa stain...

Fig. 6 BALB/c 3T3 cell transformation assay Colony formation assay...

Fig. 9 BALB/c 3T3 cell transformation assay transformation frequency of PFOA and PFNA. Red bar is the control group (untreated cells)...

Vanparys P, Schechtman L, Aardema M, Hayashi M, Hoffmann S, Gribaldo L, Corvi R (2010) Balb/c 3T3 Cell Transformation Assay - Prevalidation Study Report. ECVAM Balb/c 3T3 CTA PRP report vl - 30 July 2010... [Pg.205]

BALB/C-3T3 (cell transformation assay exposure in sealed chamber)... [Pg.42]

Ponti J, Munaro B, Fischbach M, Hoffmann S, Sabbioni E (2007) An optimised data analysis for the Balb/c 3T3 cell transformation assay and its application to metal compounds. Int J Immunopathol and Pharmacol 20(Suppl 2) 673-684... [Pg.264]

Urani C, Corvi R, Callegaro G, Stefanini FM (2013) Objective scoring of transformed foci in BALB/c 3T3 cell transformation assay by statistical image descriptors. Toxicol In Vitro 27 1905-1912... [Pg.331]

Schechtman LM (1985) BALB/c 3T3 cell transformation protocols, problems and improvements. In Kakunaga T, Yamasaki H (eds) Transformation assay of established cell lines mechanisms and application. IARC Scientific Publications 67, Lyon, pp 165-181... [Pg.205]

Matthews EJ, DelBalzo T, Rundell JO. 1985. Assays for morphological transformation and mutation to ouabain resistance of Balb/c-3T3 cells in culture. In Ashby J, de Serres FJ, et al., eds. [Pg.113]

Hazleton Labs. 1988b. Mutagenicity tests on o-cresol in the in vitro transformation of BALB/C-3T3 cells assay in the presence of rat liver cellactivation system. Unpublished data submitted to EPA/OTS. Fiche no.OTS0517697. [Pg.151]

RLV/Fischer rat assay without the addition of an exogenous metabolic activation system. In a single study, mouse JB6 epidermal cells were transformed by di(2-ethyl-hexyl) phthalate without activation and in one of two studies a weak response was reported in the CSHIOT A cell transformation assay with di(2-ethylhexyl) phthalate in either the absence or presence of exogenous metabolic activation. BALB/c-3T3 cells were not transformed by di(2-ethylhexyl) phthalate with or without metabolic activation. Di(2-ethylhexyl) phthalate inhibited gap-junctional intercellular communication in Chinese hamster V79 cells in six of seven studies, but not in one study of liver cells of cynomolgus monkeys in vivo. Di(2-ethylhexyl) phthalate treatment of Syrian hamster embryo cells in a two-stage exposure with 12-O-tetradecanoylphorbol 13-acetate resulted in superinduction of ornithine decarboxylase, an early event in morphological transformation no effect was seen after a one-stage treatment with di(2-ethylhexyl) phthalate alone. [Pg.115]

Marayama, H., Tanaka, T. Williams, GM. (1990) Effects of the peroxisome proliferator di(2-ethylhexyl)phthalate on enzymes in rat liver and on carcinogen-induced liver altered foci in comparison to the promoter phenobarbital Toxicol. Pathol., 18, 257-267 Matsushima, T, Muramatsu, M. Haresaku, M. (1985) Mutation tests on Salmonella typhimurium by the preincubation method. Prog. Mutat. Res., 5, 181-186 Matthews, E.J., DelBalzo, T. Rundell, J.O. (1985) Assays for morphological transformation and mutation to ouabain resistance of Balb/c-3T3 cells in culture. Prog. Mutat. Res., 5, 639-650... [Pg.138]

Tetrachloroethane induced gene mutations in the mouse lymphoma tH -assay only in the presence of an exogenous metabolic activation system. It did not increase the frequency of chromosomal aberrations in Chinese hamster lung fibroblasts or ovary cells but did induce sister chromatid exchanges in Chinese hamster ovary cells and aneuploidy in Chinese hamster lung fibroblasts in the absence of exogenous activation. 1,1,1,2-Tetrachloroethane did not induce cell transformation in BALB/C-3T3 cells. [Pg.1136]

Asada S, Sasaki K, Tanaka N, Takeda K, Hayashi M et al (2005) Detection of initiating as well as promoting activity of chemicals by a novel cell transformation assay using v-Ha-ras-transfected BALB/c 3T3 cells (Bhas 42 cells). Mutat Res 588 7-21... [Pg.331]

The BALB/c 3T3 cell line developed by Aaronson and Todaro has been extensively used for studies with oncogenic viruses. More recently, clones of this cell line have been used to establish quantitative focus assays in which transformation was demonstrated with several polycyclic aromatic hydrocarbons, A -acetoxy-2-acetylaminofluorene, 4-nitroquinoline-l-oxide, afla-toxin Bi, and A -methyl-A/ -nitro-A -nitrosoguanidine. Foci were not observed with diethylnitrosamine, dimethylnitrosamine, or a number of noncarcinogens. [Pg.180]

Cell Transformation Assays (in vitro) (e.g., Balb/c 3T3, C3H10T1/2, Bhas 42 cells)... [Pg.415]

ECVAM Recommendation (2012) Recommendation concerning the cell transformation assays using Syrian hamster embryo cells (SHE) and the BALB/c 3T3 mouse fibroblast cell line for in vitro carcinogenicity testing... [Pg.330]

Carcinogenicity Three cell transformation assays (CTA) (Syrian Hamster Embryo (SHE) CTA performed at pH 6.7, SHE CTA performed at pH 7.0, and BALB/c 3T3 CTA) In vitro... [Pg.666]

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