Cell transformation in vitro

Gruenert, D. C. 1987. Differentiated properties of human epithelial cells transformed in vitro. BioTechniques 5 740-50. 

The pea lectin, like con A, agglutinated normal, embryonic fibroblasts of human and rat origin at high lectin concentrations (1.500 mg/ ml), whereas, various, rat tumor-cells transformed in vitro (spontaneously, and by Rous sarcoma virus) were agglutinated at very low concentrations (5 pg/ml) of lectin.460... 

Two interferon-Y-controUed metabolic pathways accounting for some of the cytostatic effects of interferon-y in rat pleural mesothelial cells transformed in vitro with benzo[a] pyrene or chrysotile asbestos were not efficient in human mesothelioma cells, and suggested that cytokine-induced growth inhibition is mediated by a different pathway in human mesothelioma cell lines (Phan-Bitch etal. 1997). 

The adipocyte-derived hormone, adiponectin, accumulates in the injured artery from the plasma and suppresses endothelial inflammatory response and VSMC proliferation, as well as macrophage-to-foam cell transformation in vitro. In Apo E KO mice, adiponectin treatment led to inhibition of lesion formation with smaller lipid droplets in the lesions, and suppressed mRNA of VCAM-1 and class A scavenger receptor (SR). Adiponectin migrates to foam cells in the fatty streak lesions (81). 

J. A. DiPaolo, R. L. Nelson, and P. J. Donovan, Morphological, oncogenic, and karyological characteristics of Syrian hamster embryo cells transformed in vitro by carcinogenic polycyclic hydrocarbons, Cancer Res. 31, 1118-1127 (1971). 

Gallimore, P.H. 1972. Tumour production in immunosuppressed rats with cells transformed in vitro by adenovirus type 2. J. Gen. Virol. 16 99-102. 

Jensen, F., H. Koprowski, J.S. Pagano, J. Pon-ten, and R.G. Ravdin. 1964. Autologous and homologous implantation of human cells transformed in vitro by simian virus 40. /. Nat. Cancer Inst. 32 917-937. 

Isfort, R.J., Cody, D.B., Doerson, C., Kerckaert, G.A., and LeBoeuf, R.A., Alterations in cellular differentiation, mitogenesis, cytoskeleton and growth characteristics during Syrian hamster embryo cell multistep in vitro transformation, Int. J. Cancer, 59, 114, 1994. 

The results from a study employing a human cell line showed that neither 5 nor 50 ppm petroleum-derived JP-5 (PD-JP5) interfered with Snyder-Theilen feline sarcoma virus (ST-FeSV)-directed transformation of human foreskin fibroblastic cells (Blakeslee et al. 1983). Higher concentrations ( 100 ppm) were cytotoxic. It was reported that marine diesel fuel failed to inhibit transformation in this assay, but data were not shown. The study authors consider this in vitro assay to be a useful predictor of carcinogenesis since several known carcinogens have been shown to suppress transformation in cells infected with the ST-FeSV virus by blocking a specific virus gene function (i.e., transformation) noncarcinogens do not inhibit virus-induced cell transformation in this test system. 

In vitro genotoxic studies have given positive results for gene mutations, chromosomal aberrations, DNA damage, and cell transformation in the presence of metabolic activation in vivo assays have generally been negative. ... 

IP was mutagenic in bacterial assays and was positive for in vitro cell transformation in vivo it binds to mouse skin DNA. ... 

Ethylbenzene was non-mutagenic in bacteria, yeast and insects. In mammalian cells, it was inactive in inducing sister chromatid exchanges in Chinese hamster embryo cells but very weakly positive in cultured human l5miphocytes. It did not induce micronuclei in vivo, although it was positive in Syrian hamster embryo cells in vitro. It also caused cell transformation in these cells. Ethylbenzene induced mutations in the mouse lymphoma assay, but only at the highest non-lethal concentration tested. 

Diethanolamine induced cell transformation in Syrian hamster embryo cells in vitro in two studies but not in another. It did not induce gene mutations, sister chromatid exchanges or chromosomal aberrations. Diethanolamine did not induce micronucleus formation in larval newt blood cells in either the absence or presence of sodium nitrite or nitrate. It was without effect on gene conversion in yeast and was not mutagenic in bacteria. 

Chromosomal aberrations were not induced in rat liver cells, Chinese hamster lung cells or Chinese hamster ovary cells by in-vitro exposure to triethanolamine. It did not induce cell transformation in Syrian hamster embryo cells. 

Kennedy, A.R. (1984). Promotion and other interactions between agents in the induction of transformation in vitro in fibroblast-like cell culture systems, in Mechanisms of Thmor Promotion, VoL 3. Thmor Promotion and Carcinogenesis In Vitro, Slaga, T.J., Ed. (CRC Press, Inc., Cleveland, Ohio). 

Montesano, R., Saint Vincent, L., and Tomatis, L. (1973). Malignant transformation in vitro of rat liver cells by dimethylnitrosamine and N-methyl-N -nitro-N-nitrosoguanidine, Br. J. Cancer 28,215. 

Parodi, S. and Brambilla, G. (1977). Relationship between mutation and transformation frequencies in mammalian cells treated in vitro with chemical carcinogens, Mutat. Res. 47,53. 

SumMERHAYES, I.C. (1979). Influence of donor age on In vitro transformation of bladder epithelium, page 137 in Neoplastic Thansformation in Different Epithelial Cell Systems in Vitro, Franks, L.M. AND Wigley, C.B., Eds. (Acrdemic Press London). 

Hamster lung cells exposed in vitro were transformed to phenotypes that could form tumours upon transplantation either subcutaneously into newborn hamsters or intraocu-larly into adults. 

Tetrachloroethane did not induce chromosomal aberrations in Chinese hamster ovary cells. It induced sister chromatid exchanges in Chinese hamster ovary and mouse BALB/c 3T3 cell cultures and cell transformation in BALB/c 3T3 cells in vitro. 

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