Human myeloid leukemia cell line

One of the first reports showing that DAG attenuates ceramide induced apoptosis used two human myeloid leukemia cell lines, namely HL-60 and U937. Exposure of HL-60 and U937 cells to SMase or lOpM ceU-permeable ceramides for 6 h resulted in DNA-laddering, a typical hallmark of apoptosis. This could be aboUshed by di-octanoylglycerol, a cell permeable... 

Kim CH, Gollapudi S, Lee T, Gupta S (1997) Altered e ression of the genes regulating apoptosis in muitidrug resistant human myeloid leukemia cell lines overexpressing MDRl or MRP gene. Int I Oncol 11 945-950... 

Vitamin K2 (menaquinone) represents a series of compounds in which the phytyl side chain of phytonadione has been replaced by a side chain built up of 1-14 isoprenyl units. It has been reported that vitamin K2 with four isoprenyl units (4) induced the monocytic differentiation of human myeloid leukemia cell lines [58], or apoptosis in isolated osteoclast [59] and human ovary cancer cells [60]. We have recently found that vitamin K2 derivatives (1-3) induced some tumor-specific cytotoxicity and non-apoptotic cell death in oral carcinoma [61]. As an extension of the search for tumor-specific substances targeted against human oral squamous, hepatocellular carcinoma, and promyelocytic leukemia cell lines, we investigated the QSAR of seven vitamin K2 derivatives (1-7) (Fig. 18), by conventional and recent techniques of computation chemistry, such as the concept of absolute hardness [ 16-18]. 

In addition to growth inhibition and apoptosis, retinoids are well-established as redifferentiating agents in vitro. Redifferentiation and growth inhibition have been documented in osteosarcoma cell lines in vitro (Barroga et al, 1999). In human myeloid leukemia cell lines, all-tra wr-RA can induce differentiation to granulocytic- or monocytic-like cells. The same effect can be demonstrated in leukemic cells from patients with promyelocytic leukemia (Chomienne ij /., 1990). 

Human myeloid leukemia cell line (HL60 cells, ATCC 240-CCL American Type Culture Collection, Rockville, MD). Upon receipt, HL60 cells should be stored in liquid nitrogen (see Note 1). 

Anfossi G, Gewirtz AM, Calabretta B (1989) An oligomer complementary to c-myb-encoded mRNA inhibits proliferation of human myeloid leukemia cell lines. Proc Natl Acad Sci USA 86 3379-3383... 

In this study we investigated the activity of three acrylate monomers on HL-60 cells by examining of some functional parameters. Ours results clearly indicate that TEGDMA and Bis-GMA, in a dose-dependent manner, reduces cellular proliferation in HL-60 leukemia cell line. This antiproliferative effect of these monomers seems to depend on a cytostatic activity with a cytotoxic effect only at 0.6 and 0.775 mM concentrations. Moreover, the effect on cell proliferation appears higher than that observed with ATRA. HEMA, on the contrary, do not shows any effect on the cellular proliferation. All monomers induce the differentiation process of HL-60 cell line as indicated by the presence of CL activity which is strictly dependent on the appearance of respiratory burst in human myeloid tumour cell lines. 

Resveratrol has been shown to inhibit proliferation, induce differentiation, and enhance the expression of adhesion molecules (GDI la, GDI lb, GDIS, GD54) in a variety of myeloid leukemia cell lines [144]. The inhibitory effect of resveratrol on cell survival and proliferation of human breast cancer cells was shown to be estrogen receptor dependent [145]. Other studies indicated that it was very effective in inhibiting growth of 4T1 breast cancer cells in culture but had little effect on 4T1 tumor cell growth in mice [146]. In stark contrast, resveratrol was tested against mammary tumors induced by 7, 12-dimethylbenz(a)anthracene (DMBA) in rats [147]. In this case, reductions in incidence, multiplicity, and latency period of tumor development were observed. Additional... 

Cells were cultured in a humidified ahnospheric incubator at 37°C in 5% CO2. Human promyelocytic leukemia cell line (HL-60) was cultured in RPMI 1640 supplemented with 13% heat-inactivated fetal bovine serum and SO U/mL penicillin-streptomycin. To induced myeloid differentiation, cells were seeded at a density of S IO cells/mL and were cultivated for five days in RPMI 1640 containing 1.24% DMSO. TTie characteristics of mature cells were determined by smaller cell size, decreased nucleoli-to-cytoplasm ratio and pyknotic changes in nuclear chromatin. Cell numbers were counted using a hemocytometer, and cell viability was >98% as evidenced by trypan blue staining. 

HL-60 human acute myeloid leukemia cell line... 

The antiproliferative activity of 1,2,3-dithiazoles 168-176 has been tested in vitro on human myeloid leukemia K562 and L1210 murine leukemia cell lines and compared to the antiproliferative effects of the natural product distamycin A. All the imines 168-176 are active at concentration ranging from 3 to 10 pM and they retain antiproliferative activities comparable to those exhibited by distamycin A. 

Park et al. (1971) compared the effects of ascorbate on mouse plasmacytoma cells with that on normal bone marrow cells forming granulocytic colonies. Only the plasmacytoma cells responded with marked proliferation. This, however, does not imply that myeloic stem cells in general are unresponsive to ascorbate. The growth of the human promyeloic tumor cell line HL-60 was stimulated by ascorbate (Alcain et al., 1990). Similarly, bone marrow cells from a variety of acute myeloid leukemia patients responded with growth, or growth inhibition or remained unaffected (Park et al, 1992). In this context it should be stressed that inhibition of... 

Rao et al. [85] reported the molecular iodine-catalyzed reaction of 5,5-dimeth-yl-l,3-cyclohexanedione with aromatic aldehydes in iso-propanol affording a variety of 1,8-dioxo-octahydroxanthenes 61 (Scheme 10.43). Synthesized compounds were tested for in vitro antiproliferative properties against three caneer cell lines and 9-(2-hydroxyphertyl)-3,3,6,6-tetramethyl-3,4,5,6,7,9-hexahydro-l/f-xanthene-l,8(2/f)-dione possessing a 2-hydroxy phenyl group at C-9 positioa Antiproliferative properties of these eompoimds were also evaluated in vitro against a number of cancer eell lines, for example, human chronic myeloid leukemia cells (K562), human colon carcinoma cells (Colo-205), and human neuroblastoma cells (IMR32). 

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