Penicillin/streptomycin

Antibiotics such as penicillin, streptomycin, tetracyclines, chloramphenicol, and antifungals ... 

Drugs can also Interfere with laboratory results by negating certain nonspecific oxidation and reduction reactions essential for the chemical assay. Penicillin, streptomycin and ascorbic acid are known to react with cupric Ion thus, false positive results for glucose may occur If a copper reduction method Is used. If the specific enzymatic glucose-oxidase method Is employed, ascorbic acid can cause a false negative result by preventing the oxidation of a specific chromogen In the reaction. 

We have found that blood used in our fatigue tester needs to be changed about once a week. We routinely add penicillin, streptomycin, and mycostatin to the blood to prevent bacterial and... 

L-glutamine, penicillin, streptomycin and gentamycin (Gibco, Gent, Belgium) at the usual concentrations. 

Colony forming ability of the fetal liver cells was determined in the medium comprised 1.3% methylcellulose, 4.0 mM glutamine, 10 U/ml penicillin/-streptomycin, 100 U/ml GM-CSF, 100 U/ml IL-3, 50 ng/ml stem cell factor and 10 U/ml erythropoietin in IMDM. An aliquot of 10 cells was transferred to a 35 mm sterile plastic Petri dish and incubated at 37 C in a fully humidified atmosphere of 5% CO2 in air. The final colony count was performed on day 14 of culture, the colony types being defined by general morphological criteria. 

Lincomycin Neomycin Novobiocin Nystatin Oxytetracycline Penicillin Streptomycin Tylosin... 

The conventional bioassays based on methodology developed by FDA and expanded by FSIS use four extractant buffers, five test organisms, five growth media, two incubation temperatures, and penicillinase to detect, identify, and/or quantify antibiotics such as the penicillins, streptomycins, tetracyclines, neomycins, erythromycin, tylosin, etc. Bioassay laboratory results are used by FSIS to take regulatory action and by FDA to prosecute farmers with histories of improperly withdrawing antibiotics before marketing their herds or flocks. 

Penicillin-Streptomycin mixed solution (Nacalai Tesque, 26253-84). 

Horse serum Glutamine solution Penicillin Streptomycin M199... 

Primary fibroblasts from skin biopsy samples are grown at 37°C in the presence of 5% C02 in Dulbecco s modified Eagle s medium (DMEM) supplemented with 10% foetal calf serum (FCS) and 1% penicillin/streptomycin. For passaging, confluent cells are washed with phosphate-buffered saline (PBS) and incubated for 5-10 min at 37°C in the presence of trypsin. 

A number of additives have, since the original feed additives list in 1970, been withdrawn from the list of authorized additives for different reasons including their contribution to the generation of resistance to bacteria (avoparcin, tylo-sin, virginiamycin), and the decision to restrict certain antibiotics to therapeutic use (penicillin, streptomycin, tetracyclines). Until recently there has been an incoherence between feed additive and veterinary medicine legislation in the European Union since use of some agents such as dimetridazole and ronidazole was... 

In the period 1988-1989, the drug residue profile in edible animal products does not change. Data complied by the FSIS National Residue Program during 1988 showed that chlortetracycline, erytlrromycin, gentamicin, neomycin, oxytetracycline, penicillins, streptomycin, tetracycline, and tylosin were the antibiotics most often present in the samples analyzed (Table 13.3). During the same period. 

Other chemicals which inhibit milk lipase include hydrogen peroxide, animal cephalin, sodium arsenite, diisopropyl fluorophosphate, 2,4 din-itro-l-fluorobenzene, p-hydroxymercuribenzoate, potassium dichromate, lauryl dimethyl benzyl ammonium chloride, aureomycin, penicillin, streptomycin, and terramycin (Schwartz 1974). 

Diluted antibody solutions can be reused multiple times They should be stored at 4°C after additional aliquots of penicillin/streptomycin and sodium azide have been added Some workers believe that the amount of nonspecific (background) staining on Western blots diminishes as antibody solutions are reutilized Antibody solutions are discarded or supplemented with additional antibody when the intensity of the specific signal begins to diminish 10. Choice of wash buffer after incubation with primary antibody 2M urea is included m the suggested wash buffer to diminish nonspecific binding. Alternatively, some... 

Dr. Tishler published more than 100 scientific papers and is cited as an inventor on more than 100 United States patents. A partial list of research contributions include development of processes for the commercial production of vitamin B6, vitamin K, vitamin E, penicillin, streptomycin, and cortisone. 

Media basal RPMI 1640 medium requires supplementation with sodium pyruvate, L-glutamine, and penicillin/streptomycin before use. The supplements are supplied as concentrates of 50X or 100X, and the appropriate amounts should be added. Standard tissue culture media for hybridoma production contain 5%, 10%, or 15% fetal bovine serum (FBS see Note 6). Sufficient quantities should be prepared in advance and a sterility check should be performed on them prior to use. 

Dulbecco s modified Eagle s medium (DMEM) with L-glutamine and 4.5 gm/L glucose. Add penicillin-streptomycin (Gibco BRL, Gaithersburg, MD) from 100X stock. Add fetal bovine serum (FBS) to a final concentration of 10%. 

NIH3T3 culture medium DMEM (Gibco) supplemented with 10% new born calf serum (Lonza, Cat 14-416F), 1% penicillin-streptomycin solution (Gibco), 1% L-glutamine solution (Gibco). 

Bone marrow cell first time stimulation medium 77% (v/v) DMEM, 15% (v/v) heat inactivated FBS, 5% (v/v) WEHI-3B conditioned medium, penicillin-streptomycin, 1.0 m g/mL ciprofloxacin, 200 mM L-glutamine, 6 ng/mL recombinant murine IL-3 (Peprotech, Cat 213-13), 10 ng/mL recombinant murine IL-6 (Peprotech, Cat 216-lb), and 50-100 ng/mL recombinant murine stem cell factor (SCF Peprotech, Cat 250-03). The total volume is 10 mL for each sample. For the second round stimulating medium, the volume is 4 mL for each sample. 

Antibiotics such as penicillin, streptomycin, and amphotericin B can also be added to the culture medium to prevent microbial growth. However, their use should be kept to a minimum level, because some antibiotics can have cytotoxic effect, even when high serum concentrations are used. Besides, the continuous use of antibiotics can result in the selection of resistant microorganisms, inducing a chronic contamination of the culture (Freshney, 1992). 

When some foodstuffs like sugar or medicines (penicillin, streptomycin and other antibiotics), it is necessary to evaporate large quantities of liquids to extract the diluted substance in the crystallic form. The evaporation forms a lot of foam, which interferes with the process, causes losses of the products and makes it necessary to increase the volume of the apparatuses. Experiment shows that a neglible amount of silicone liquid added to these solutions (1 weight part per 100 thousand or even 1 min of weight parts of the solution) completely prevents the formation of foam during evaporation. 

Rinse each uterus with 25 ml of PBS-A containing 1% penicillin/streptomycin. 

Seed with 2 X 106 (9-cm dish) or 105 (TC well) cells in 10 ml or 0.5 ml Eagle s medium containing non-essential amino acids, penicillin-streptomycin and 10% foetal bovine serum (Appendix 1). 

HEK 293 cells are grown in Dulbecco s MEM / Nut Mix F-12 supplemented with 2 mM L-glutamine, 100 U/mL penicillin-streptomycin and 10% heat-inactivated fetal bovine serum (FBS complete medium). 

Medium 500 mL Dulbecco s Modified Eagle s Medium (DMEM) containing 10% fetal bovine serum (50 mL FBS) and 1% Penicillin-Streptomycin (5 mL) Invitrogen... 

HeLa cell cultures are propagated in Dulbecco s Modified Eagle Medium (D-MEM) (500 mL) (Gibco) supplemented with 10% fetal bovine semm (FBS, Bio West), 5 mL MEM non-essential amino acids (100 x ) (Gibco), 5 mL sodium pyruvate MEM (100 m /. Gibco), and 5 mL Penicillin-Streptomycin-Neomycin (PSN) Antibiotic Mixture (Gibco). 

HeLa cells grown in DMEM with 10% FBS supplemented with glutamine and-penicillin/streptomycin (Invitrogen). 

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