Alkaline elution

Bradley MO. 1985. Measurement of DNA single-strand breaks by alkaline elution in rat hepatocytes. In Ashby J, de Serres FJ, et al., eds. Progress in mutation research. Vol. 5. Evaluation of short- term tests for carcinogens. Amsterdam, The Netherlands Elsevier Science Publishers, 353-357. 

Hachiya N, Sato M, Takizawa Y. 1984. Detection of DNA damage in mutagen-treated mammalian tissues by alkaline elution assay. Mutat Res 130 363. 

Parodi, S., M.Taningher, P.Russo, M.Pala, M.Tamaro, and C.Monti-Bragadin. 1982. DNA damage in liver, kidney, bone marrow and spleen of rats and mice treated with commercial and purified aniline as determined by alkaline elution assay and sister chromatid exchange induction. Cancer Res. 42 2277—2283. 

Monomethylhydrazine-induced mutagenesis was not observed in Ames Salmonella/ microsome with activation (Matheson et al. 1978). In vivo tests in mice (dominant lethal, revertants in host-mediated assay), and dogs (micronuclei) were negative (reviewed in Trochimowicz 1994). However, in vitro chromosomal damage in human and rat tissue has been demonstrated, although in vivo liver DNA damage (as determined by DNA alkaline elution) was equivocal (reviewed in Trochimowicz 1994). 

Administration of single oral doses of 90 or 120 mg/kg mirex by gavage to female Sprague-Dawley rats resulted in induction of hepatic ornithine decarboxylase activity there was, however, no evidence of significant damage to deoxyribonucleic acid (DNA) as measured by alkaline elution (Mitra et al. 1990). 

Skare JA, Schrotel KR. 1984. Alkaline elution of rat testicular DNA Detection of DNA strand breaks after in vivo treatment with chemical mutagens. Mutat Res 130 283-294. 

White RD, Sipes IG, Gandolfi AJ, et al. 1980. Determination of ethylene bromide induced DNA injury by alkaline elution. Fed Proc Fed Am Soc Exp Biol 39 1022. 

Boekelheide, K. (1993) Sertoli cell toxicants. In Russell, L.D. Griswold, M.D., eds. The Sertoli Cell, Clearwater, FL, Cache River Press, pp. 551-575 Bove, J.L., Dalven, P. Kukreja, V.P (1978) Airborne di-butyl and di(2-ethylhexyl)phthalate at three New York City air sampling stations. Int. J. environ. Chem., 5, 189-194 Bradley, M.O. (1985) Measurement of DNA single-strand breaks by alkaline elution in rat hepatocytes. Prog. Mutat. Res., 5, 353-357... 

Brambilla, G, Carlo, R, Finollo, R. Sciaba, L. (1987) Dose-response curves for liver DNA fragmentation induced in rats by sixteen A-nitroso compounds as measured by viscometric and alkaline elution analyses. Cancer Res., 47, 3485-3491... 

Vangala, R.R., Laib, R.J. Bolt, H.M. (1993) Evaluation of DNA damage by alkaline elution technique after inhalation exposure of rats and mice to 1,3-butadiene. Arch. Toxicol., 67,34-38... 

Bradley. M.O. (1985) Measurement of DNA single-strand breaks by alkaline elution in rat... 

Barbin, A., Bereziat, J.C. Bartsch, H. (1983) Evaluation of DNA damage by the alkaline elution technique in liver, kidneys and lungs of rats and hamsters treated with V-nitrosodialkyl-amines. Carcinogenesis, 4, 541-545... 

Schwarz, M.. Hummel, J., Appel, K.E., Rickert, R. Kunz, W. (1979) DNA damage induced in vivo evaluated with a non-radioactive alkaline elution technique. Cancer Lett.. 6, 221-226... 

Sina, J.F., Bean, C.L., Dysart, G.R., Taylor, V.I. Bradley, M.O. (1983) Evaluation of the alkaline elution/rat hepatocyte assay as a predictor of carcinogenic/mutagenic potential. Mutat. Res., 113, 347-391... 

Swenberg, J.A. (1981) Utilization of the alkaline elution assay as a short-term test for chemical... 

Brunborg, G. Holme. J.A.. Soderlund, E.J., Omichinski. J.G. Dybing. E. (1988) An automated alkaline elution system DNA damage induced by 1.2-dibro mo-3-chloropropane in vivo and in vitro. Anal. Biochem.. 174. 522-536... 

Swenberg, J.A. (1981) Utilization of the alkaline elution assay as a short-term test for chemical carcinogens. In Stich, H.F. San, R.H.C.. eds, Short-Term Tests for Chemical Carcinogens, New York, Springer, pp. 48-58... 

Ethylene dibromide caused a dose-dependent increase in liver DNA alkaline-labile sites and single-strand breaks (as determined by alkaline elution assay) in female Sprague-Dawley rats. It was positive in an unscheduled DNA synthesis assay in rat spermatocytes in vitro but was negative in the spermatocytes of rats dosed in vivo. Ethylene dibromide gave positive results in an amphibian Pleurodeles yvaltl) mieronueleus test but gave negative results in dominant lethal tests. 

White, R.D., Sipes, I.G, Gandolfi, A.J. Bowden, GT. (1981) Characterization of the hepatic DNA damage caused by 1,2-dibromoethane using the alkaline elution technique. Carcinogenesis, 2, 839-844... 

DIA, DNA single strand breaks, cross-links or related damage, LYS mouse lymphoma cells, alkaline elution in vitro... 

Phenol did not induce DNA single-strand breaks in mouse lymphoma L5178Y cells. It was reported in abstracts that phenol induced DNA strand breaks in mouse lymphoma cells, as measured by the alkaline unwinding technique followed by elution through hydroxyapatite (Garberg Bolesfoldi, 1985), but that it did not induce strand breaks, as measured by the alkaline elution technique, in rat germ-cell DNA after either single or multiple dose treatments (Skare Schrotel, 1984). 

Sega, G.A., Sluder, A.E., McCoy, L.S., Owens, J.G. Generoso, E.E. (1986) The use of alkaline elution procedures to measure DNA damage in spermiogenic stages of mice exposed to methyl methanesulfonate. Mutat. Res., 159, 55-63... 

DNA strand breaks and related damage (alkaline elution assay) in - 1500 ipx 1 Longneeker et al. (1990)... 

As already indicated, strand breaks can be detected and measured by alkaline sucrose-gradient centrifugation and by alkaline elution. Both procedures have been applied to cells from intact animals, as well as to in vitro systems. 

Alkaline elution of large DNA fragments from cells lysed on filters was devised by Kohn and colleagues215 216 and has been applied to the study of damaging effects on DNA of carcinogens given to animals in vivo. As with the... 

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