Human embryonic kidney

FIGURE 2.11 Receptor-occupancy curves for activation of human calcitonin type 2 receptors by the agonist human calcitonin. Ordinates (response as a fraction of the maximal response to human calcitonin). Abscissae (fractional receptor occupancy by human calcitonin). Curves shown for receptors transfected into three cell types human embryonic kidney cells (HEK), Chinese hamster ovary cells (CHO), and Xenopus laevis melanophores. It can be seen that the different cell types lead to differing amplification factors for the conversion from agonist receptor occupancy to tissue response. 

Human embryonic kidney cells, 21 Human genome, 2 Hydrogen bonding, 10 Hypothesis testing definition of, 239 description of, 227, 233 dose-response curves for, 239-243 F-test, 242t... 

Fig. 3. Comparison between IP-One kit versus calcium mobilization assay (384-well format). Human embryonic kidney (HEK) 293 cells expressing a chemokine receptor were evaluated on HTRF IP-One kit (CisBio, Bedford, MA) and fluroescent imaging plate reader (FLIPR) with Calcium 3 kit (Molecular Devices, Mountain View, CA).

Wang, F., Nelson, M., Kuryatov, A. et al. Chronic nicotine treatment upregulates human a3 32 but not a3P4 acetylcholine receptors stably transfected in human embryonic kidney cells. J. Biol. Chem. 1998 28721, 1998. 

McAleer MA, Breen MA, White NL, Matthews N. pABCll (also known as MOAT-C and MRP5), a member of the ABC family of proteins, has anion transporter activity but does not confer multidrug resistance when overexpressed in human embryonic kidney 293 cells. J Biol Chem 1999 274(33) 23541-23548. 

In addition to direct enhancement of channel activity, PTKs can indirectly increase GABA-evoked inhibitory current by recruiting intracellular GABAaR to the surface of postsynaptic membrane. Insulin has been shown to increase surface expression GABAaR in transfected human embryonic kidney cells. In central neurons insulin rapidly increases the expression of functional postsynaptic GABAaR in a tyrosine kinase-dependent manner, resulting in an increase in the amplitude of the miniature inhibitory postsynaptic currents. 

Galli, A., Jayanthi, L. D., Ramsey, I. S., Miller, J. W., Fremeau, R. T Jr., and DeFelice, L. J. (1999) L-proline, and L-pipecolate induce enkephalin-sensitive currents in human embryonic kidney 293 cells transfected with the high-affinity mammalian brain L-proline transporter. J. Neurosci. 19, 6290-6297. 

Recent results that examined the effect of pyrethroids on voltage-gated sodium channels expressed in human embryonic kidney (HEK293) cells grown in... 

He B, Soderlund DM (2010) Human embryonic kidney (HEK293) cells express endogenous voltage-gated sodium currents and Nav1.7 sodium channels. Neurosci Lett 469 268-272... 

PR. Wielinga, G. Reid, E.E. Challa, I. van der Heijden, L. van Deemter, M. de Haas, C. Mol, A.J. Kuil, E. Groeneveld, J.D. Schuetz, C. Brouwer, R.A. De Abreu, J. Wijnholds, J.H. Beijnen, and P. Borst. Thiopurine metabolism and identification of the thiopurine metabolites transported by MRP4 and MRP5 overexpressed in human embryonic kidney cells. Mol Pharmacol. 62 1321-1331 (2002). 

Non-polarising BHK CHO Cos-1, Cos-7 HEK-293 (Baby) Syrian hamster kidney, fibroblast-like Chinese hamster ovary, fibroblast-like African green monkey kidney, fibroblast-like Human embryonic kidney, epithelial... 

Figure 22. Human embryonic kidney cells (A), rat vascular smooth muscle cells (B, C) and human osteoblast-like MG 63 cells (D) in cultures on micropattemed surfaces. A, B PTFE irradiated with UV light produced by a Xe2 -excimer lamp for 30 min in an ammonia atmosphere through a mask with holes 100 pm in diameter and center-to-center distance 300 pm C PE irradiated with Ar ions (energy 150 keV, ion dose lO ions/cm ) through a mask with holes 100 pm in diameter and center-to-center distance 200 pm fullerenes Qo deposited through a mask with rectangular holes with an average size of 128 3 pm per 98 8 pm on glass coverslips. Day 7 after seeding. A native cells in an inverted phase-contrast microscope B, C cells stained with hematoxylin and eosin, Olympus microscope IX 50 D cells stained with fluorescence-based LIVE/DEAD viability/cytotoxicity kit (Invitrogen), Olympus microscope IX 50. Bars 300 pm (A), 200 pm (B, D), Imm (C) [10,11].

Cui et al. [19] SWCNT Human embryonic kidney cells (HEK 293) in solution with 0.78-200 mg/ mL of SW CNT Induction of apoptosis and reduction of adhesion ability (and corresponding genes), reduction of cellular proliferation... 

Interactions with drugs have also been demonstrated for flavonoids from citrus. Naringin and naringenin were shown to interact with simvastatin and saquinavir in in vitro experiments (41,42) and caused alterations in the pharmacokinetics of quinine in rats (44). Moreover, naringenin and naringin were found to inhibit the OATP-B-mediated uptake of estrone-3-sulfate into human embryonic kidney cells (23). 

Regarding interactions between citrus and OATP, data from in vitro, animal and also human clinical studies are available. These studies demonstrated the inhibition of OATP-A in HeLa cells and of OATP-B mediated uptake of estrone-3-sulfate in human embryonic kidney cells and also in the oral intake of fexofenadine in rats by fruit juices, including GFJ and orange juice. Human clinical trials suggest a potential role of OATP in grapefruit-drug interactions using fexofenadine as substrate (22,23,67,78,79). 

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