Human colonic cell line

RT Worrell, AG Butt, WH Cliff, RA Frizzell. (1989). A volume-sensitive chloride conductance in human colonic cell line T84. Am J Physiol 256 C1111-C1119. 

Delie, F. and W. Rubas. A human colonic cell line sharing similarities with enterocytes as a model to examine oral absorption advantages and limitations of the Caco-2 model, Crit. Rev. Ther. Drug Carrier Syst. 1997, 34, 221-286... 

Howell, D., Kenny, A. J., Turner, J., A survey of membrane peptidases in two human colonic cell lines, Caco-2 and HT29, Biochem. J. 1992, 284, 595-601. 

Collett, A., Higgs, N. B., Sims, E., Rowland, M., Warhurst, G., Modulation of the permeability of H2 receptor antagonists cimetidine and ranitidine by P-glycoprotein in rat intestine and the human colonic cell line Caco-2, J. Pharmacol. Exp. Ther. 1999, 288, 171-178. 

Bonnesen C, Eggleston I M and Hayes J D (2001), Dietary indoles and isothiocyanates that are generated from cruciferous vegetables can both stimulate apoptosis and confer protection against DNA damage in human colon cell lines , Cancer Rese, 61, 6120-6130. 

A. I. Haza, B. Glinghammar, A. Grandien and J. Rafter, Effect of colonic luminal components on induction of apoptosis in human colonic cell lines, Nutr. Cancer, 2000, 36(1), 79. 

Fucolipids and Gangliosides of Human Colonic Cell Lines... 

Cell Lines. The human fetal intestinal cell line (FHS) was kindly supplied to us by Dr. Walter A. Nelson-Rees at the Naval Bioscience Laboratory, Oakland, California. The human colonic cell line, SKCO-1 developed by Drs. G. Trempe and L.F. Olds, was obtained from Dr. Jorgen Fogh, Sloan Kettering Institute, Rye, New York. The HT-29 cell line was developed and obtained from Dr. J. Fogh. SW-480 and SW-620 were developed at Scott and White Clinic in Temple, Texas and were obtained from Col. A. Liebovitz. 

Ham M. and Kaunitz J.D. (2007) Gastroduodenal defense. Curr Opin Gastroentrol 23, 607-616 Hidaka E., Ota H. and Hidaka H. (2001) Helicobacter pylori and two ultrastructurally distinct layers of gastric mucous cell mucins in the surface mucous gel layer. Gut 49, 474-480 Hong D.H., Forstner J. and Forstner G. (1997) Protein kinase C-e is the likely mediator of mucin exocytosis in human colonic cell lines. Am J Physiol Gastrointest Liver Physiol 272,G31-G37... 

Palominol. Corey has reported the application of an intramolecular titanium-mediated pinacol coupling reaction to the synthesis of a 15-membered ring, en route to palominol, a marine diterpenoid that displays cytotoxicity toward the human colon cell line (Eq. 3.10) [28]. Slow addition of the keto aldehyde (32 h) to the titanium reagent furnished the cyclized product in 53% yield as a mixture of diastereomers (2.1 1). 

Diallyl sulfide (DAS) Diallyl disulfide (DAOS) Diallyl trisulfide (DATS) inhibition of BP-induced fore-stomach, lung and liver cancer in mice, increase hepatic and fore-stomach GST and EROO activities, inhibition of tumors in human colon cell line (DADS only), anti-mutagenic, antihepatotoxic. 102,105, 107-111. 

The most commonly used cell culture model today is the 21-day Caco-2 human colonic cell line derived from a human adenocarcinoma. They can be cultured in special transwell cell culture plates (Figure 9) that enable the investigation of passive diffusion (apical to basolateral side) and active transport (basolateral to apical side). Although the system has its limitations, for many compounds it can give a good indication of likely in vivo absorption. An alternative cell culture, which has been shown to correlate well with absorption in vivo and permeability in Caco-2 cultures, is the 3-day Madin-Darby canine kidney cell line. Also, the expression of transporter proteins in cell cultures has led to new screens being established for identifying transporter substrates. 

Conjugated Linoleic Acid Inhibits Cell Proliferation and ErbB3 Signaling in HT-29 Human Colon Cell Line, Am. Physiol. 284 G996—1005 (2003). 

Kalkitoxin showed potent cytotoxicity against the human colon cell line HCT-116 (IC50 1.0 ng/mL) [7]. In a zone differential cytotoxicity assay, kalkitoxin showed differential cytotoxicity for a solid tumor cell (Colon 38) versus... 

Adlerberth, I., Ahrne, S., Johansson, M.L., et al. (1996) A mannose-specific adherence mechanism in Lactobacillus plantarum conferring binding to the human colonic cell line HT-29. Appl Environ Microbiol 62,2244-2251. 

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