Cell culture human cells

Mammalian cells Cultured human lymphocytes Mitotic recombination Aberrant metaphases No data - L Vova 1984... 

In view of the fact that large volumes have been administered to man without causing serious side effects (Zonneveld and Crommelin, 1988), it may be concluded that although liposomes can damage in vitro-cultured human cell lines (Nuzzo et al., 1985 Mayhew et al., 1987b), adverse effects observed in vivo are expected to be minimal. One should realize that liposomes reduce the toxicity of the encapsulated drug. Thus, the toxicity of liposomes may be of minor importance compared to the advantages of administration of certain chemotherapeutics encapsulated in liposomes. 

Mayhew, E., Ito, M., and Lazo, R. (1987b). Toxicity of non-drug containing liposomes for cultured human cells, Exp. Cell Res.. 

Hurst, JS, Saini, MK, Jin, GF, Awasthi, YC, and van Kuijk, FJ, 2005. Toxicity of oxidized beta-carotene to cultured human cells. Exp Eye Res 81, 239-243. 

It has been reported that ceruloplasmin increases iron uptake into cultured human cells (Mukhopadhyay et al, 1998), although this apparently contradictory in vitro observation must be set against the in vivo evidence presented above from both animal and human studies. Plasma ceruloplasmin levels increase markedly in anaemia, consistent with a physiological role in tissue iron mobilization, and this effect is due to transcriptional activation of ceruloplasmin mRNA synthesis (Mukhopadhyay et al, 2000). 

Fogh, J., Fogh, J. M., Oreeo, T., One hundred and twenty-seven cultured human cell lines producing tumors in nude mice, J. Natl. Cancer Inst. 1977, 59, 221-225. 

Finally, GTP is bound by many proteins, and diazirine-bearing thiol-cleavable GTP analogs (e.g., 23) have been proposed as probes to detect such proteins. By this method, H-Ras was successfully labeled in a nuclear extract of cultured human cells [70]. 

Ahmed FE, Hart RW, Lewis NJ. 1977. Pesticide induced DNA damage and its repair in cultured human cells. Mutat Res 42 161-174. 

Figure 27. Human osteoblast-like MG 63 cells in cultures on porous (A) or fibrous (B) poly(L-lactide-co-glycolide) scaffolds. A A summarizing picture of horizontal optical sections. The depth of cell ingrowth into the pores (average pore diameter of 400-600 mm) is indicated by spectral colors (blue 0-60 mm, green 80-160 mm, yellow 180-220 mm, orange 240-300 mm, red 320-400 mm, violet 420-480 mm). Day 14 after seeding, cells stained with propidium iodide. B cells grown for 4 days in static culture followed by 2 days in dynamic perfusion cell culture system. Cell membrane stained with Texas Red C2-maleimide and the nuclei counterstained with Hoechst 33342. Leica TCS SP2 confocal microscope, objective 5x (A) or lOx (B) [37].

Ali-Khan, Z., Chan, S.L., Jung, S.S., Chronopoloulos, S. (1996) Ubiquilin and Alzheimer s amyloid beta precursor protein colocalize to endosomes-lysosomes in cultured human cells. Neuroreport, 8, 385-389. 

Thiram was genotoxic to insects, plants, fungi, and bacteria it induced sister chromatid exchange and unscheduled DNA synthesis in cultured human cells. Despite established genotoxicity in vitro, it showed no clastogenic and/or aneugenic activity in vivo after oral administration to mice at the maximum tolerated dose. ... 

It has a cytocidal effect on both proliferating and nonproliferating cultured human cells, suggesting lack of cell cycle phase specificity. 

Myriad Genetics reports that Flurizan is a selective amyloid-lowering agent (SALA) that reduces levels of the peptide amyloid beta 43 in cultured human cells and in animal models. Results of a completed Phase II follow-on study of Flurizan in patients with mild AD were presented in July 2006 at the International Conference on Alzheimer s Disease and Related Disorders. According to Myriad Genetics, results from this study reportedly... 

Roe CR, Roe DS (1999) Recent developments in the investigation of inherited metabolic disorders using cultured human cells. Mol Genet Metab 68 243-257... 

Palmer, T.D., Schwartz, P.H., Taupin, P., Kaspar, B., Stein, S.A., Gage, F.H. (2001). Cell culture. Progenitor cells from human brain after death. Nature, 411, 42-3. 

Cell culture. Progenitor cells from human brain after death. Palmer, T.D., Schwartz,... 

Budavari, S., ed. (1996) The Merck Index. 12th Ed.. Whitehouse Station, NJ, Merck Co., p. 648 Chang, T.H. Elequin, F.T. (1967) Induction of cliromosomc aberrations in cultured human cells... 

The product yields in y-irradiated DNA are given in Tables 12.5-12.7. FAPY-G has always been observed, but 8-oxo-G yields were extremely low, when y-ir-radiations were carried out under N2. This may serve as a caveat for the common practice to use 8-oxo-G as a kind of marker for free-radical DNA damage (for assays see Chap. 13.2). Besides the other products reported in Table 12.5, 8 cG lesions per 106 bases are formed per Gy in y-irradiated N20-saturated DNA (50 pg ml-1) solution (Dizdaroglu et al. 2001a corresponding to 1.6 x 10 9 mol J-1), and this lesion has also been observed in y-irradiated cultured human cells (Dizdaroglu 1986 for its elimination by nucleotide excision repair see Kuraoka et al. 2000). 

Cell Culture. Human neuroblastoma IMR-32 (passaged through nude mice the cells were donated by Dr. Steven E. Brooks, Kingsbrook Jewish Medical Center, Brooklyn) and mouse neuroblastoma clones NIE-115, NS-20, and N-18) (donated by Dr. Shraga Makover, Hoffmann LaRoche, Inc., Nutley, New Jersey) were maintained in our laboratory as described previously (30,31). Confluent monolayers (6 to 8 x 10 cells per 250-ml Falcon plastic flask) were harvested for enzymatic studies with phosphate-buffered saline [7.0 mM potassium phosphate/0.14 M NaCl - buffer, pH 7.2 (Pi/NaCl)] containing 0.1% EDTA. 

Holladay, S. R., Yang, Zhen-fan, Kennedy, M. D., et al. Riboflavin-mediated delivery of a macromolecule into cultured human cells. Biochim. Biophys. Acta 1426 195-204, 1999. 

E. coli bacteria4S) and in cultured human cells. In Fig. 21 the survival curves are given for human fibroblasts after treatment with various cispiatin concentrations50 The curves obtained for cells of patients suffering from inherited DNA repair deficiencies, such as xeroderma pigmentosum and Fanconi s anemia, clearly indicate that these cells are more susceptible to the cytotoxic activity of cispiatin than the same type of cells obtained from normal , healthy persons. 

In vitro genotoxicity studies are summarized in Table 2-17. Eukaryotic cell systems were used for detecting the effects of 2,3,7,8-TCDD exposure on DNA. Exposure to 2,3,7,8-TCDD did not stimulate the unscheduled DNA synthesis in cultural human cells (Loprieno et al. 1982), but inhibited DNA, ribonucleic acid (RNA), and protein synthesis in mouse lymphocytes (Luster et al. 1979) caused gene mutations in mouse lymphoma cells (Rogers et al. 1982) and induced sister chromatid exchanges in Chinese hamster cells (Toth et al. 1984). 

Cell Culture Human colon cancer cells (LoVo) were obtained from Dr. M. Lipkin (Cornell University Medical Center) and human breast cancer cells (MCF-7) were obtained from Dr. 

Therefore, test systems must be designed to mimic the metabolism of the human body. Extracts from rat liver have proved to be highly effective, and many substances have been found to be mutagenic in bacteria and cultured human cells only when such activating systems are included. Mutation tests are run both with and without activation, to detect chemicals that are already mutagenic and might be inactivated, as well as those which require activation to become mutagenic. 

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