Neuroblastoma IMR-32, human

These compounds block N-type calcium channels in human neuroblastoma IMR-32 cells. PD-151307 (IC50 = 0.22 pM) shows about 40-fold selectivity for N- over L-type calcium channels (IC50 = 9.1 pM in GH3 cells). These compounds may also be potentially useful in the treatment of cerebral ischemia and chronic intractable pain. 

Cell Culture. Human neuroblastoma IMR-32 (passaged through nude mice the cells were donated by Dr. Steven E. Brooks, Kingsbrook Jewish Medical Center, Brooklyn) and mouse neuroblastoma clones NIE-115, NS-20, and N-18) (donated by Dr. Shraga Makover, Hoffmann LaRoche, Inc., Nutley, New Jersey) were maintained in our laboratory as described previously (30,31). Confluent monolayers (6 to 8 x 10 cells per 250-ml Falcon plastic flask) were harvested for enzymatic studies with phosphate-buffered saline [7.0 mM potassium phosphate/0.14 M NaCl - buffer, pH 7.2 (Pi/NaCl)] containing 0.1% EDTA. 

In order to obtain some idea about the nature of gly-coconjugates and their gross topographical orientation on the cell surfaces, we measured the binding of 125j labeled lectins and toxin to human neuroblastoma IMR-32 and mouse neuroblastoma N1E-115, NS-20, and N-18 clones (Tables VII and VIII). The "5% TCA Wash" column (Table VII) represents 1251-iabeled lectin or toxin bound to both glycoprotein and glycolipid. The "5%... 

Lee, J.M., Hanson, .M., et ah, Phosphatidylinositol 3-kinase, not extracellular signal-regulated kinase, regulates activation of the antioxidant-responsive element in IMR-32 human neuroblastoma cells, J. Biol. Chem., 276, 20011-20016, 2001. 

Lee JM, Moehlenkamp JD, Hanson JM, Johnson JA (2001) Nrf2-dependent activation of the antioxidant responsive element by tert-butylhydroquinone is independent of oxidative stress in IMR-32 human neuroblastoma cells. Biochem Biophys Res Commun 280 286-292... 

A. Takahara, S. Fujita, K. Moki, Y. Ono, H. Koganei, S. Iwayama, and H. Yamamoto. Neuronal calcium channel blocking action of an antihypertensive drug, Cilinidipine, in IMR-32 human neuroblastoma cells. Hypertension Research 26 743-747 (2003). 

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