Recombination mitotic

MITOTIC RECOMBINATION Mitotic crossing-over and mitotic gene conversion. 

Assay for mitotic recombination in yeast and Drosophila Assay for unscheduled DNA synthesis in cultured hepatocytes and rodents... 

Mammalian cells Cultured human lymphocytes Mitotic recombination Aberrant metaphases No data - L Vova 1984... 

S. cerevisiae Gene conversion or mitotic recombination — — Fukunaga et al. 1982 Kharab and Singh 1985 Nestmann et al. 1979 Simmon 1979a... 

Saccharomyces cerevisiae mitotic recombination assay OECD... 

OECD 482] Mitotic recombination in Saccharomyces cerevisiae (yeast) / ... 

Katz AJ. 1987. Inhalation of methyl bromide gas induces mitotic recombination in somatic cells of Drosophilla melanogaster. Mutat Res 192 131-135. 

Genetic Toxicology Saccharomyces cerevisiae, Mitotic Recombination Assay (Original Guideline, adopted 23 October 1986)... 

The term genotoxicity is a broader term and refers to potentially harmful effects on genetic material, which are not necessarily associated with mutagenicity. Thus, tests for genotoxicity include tests, which provide an indication of induced damage to DNA (but not direct evidence of mutation) via effects such as unscheduled DNA synthesis (UDS), sister chromatid exchange (SCE), DNA strand breaks, DNA adduct formation or mitotic recombination, as well as tests for mutagenicity. ... 

Genetic toxicology Saccharomyces cerevisiae, mitotic recombination assay 1986... 

The Saccharomyces cerevisiae mitotic recombination assay OECD TG 481 US-EPA OPPTS 870.5575 EU Annex V B.16... 

Positive results from the Saccharomyces cerevisiae mitotic recombination assay indicate that a substance induces DNA recombination in this test system, i.e., mitotic gene conversion (within a gene) and/or mitotic crossing-over (between a gene and its centromere). 

DNB on mitotic recombination in Saccharomyces cerevisiae. Doses of up to 32 mg/mL were administered, but no genotoxic effects of any kind were observed either with or without metabolic activation (McGregor et al. 1980). Refer to Table 2-2 for a further summary of the genotoxic effects of 1,3-DNB exposure. 

As shovm in Figure 19.4, the D7 strain of S. cerevisiae is a particularly useful tool because of its ability to assess various genotoxic events such as intergenic and intragenic mitotic recombination as well as point mutagenesis [37, 38[. In a recent paper, we showed that the D7 yeast strain could detect the phototoxicity of most of chemicals used in the validated phototoxicity test 3T3 NRU, except the antibiotics... 

Fortunately, a number of in situ, short-term bioassays to detect genotoxic and related effects have become available. These include a variety of measured endpoints such as aneuploids, chromosal aberrations, DNA damage, dominant lethal mutation, gene mutation, inhibition of intercellular communication, micronuclei, mitotic recombination and gene conversions, and sister chromatid exchange and cell transformation (IARC, 1989). A detailed discussion of these tests is beyond the scope of this book. However, such tests are important from our perspective as atmospheric chemists because, as we shall see, they can be used to detect biologically active compounds in very complex mixtures, and hence serve to focus chemical analysis efforts (IARC, 1989, p. 20). We emphasize in advance the... 

Sinigaglia M, Reguly ML, de Andrade HH (2004) Effect of vanillin on toxicant-induced mutation and mitotic recombination in proliferating somatic cells of Drosophila melanogaster. Environ Mol Mutagen 44 394-400... 

A small increase in somatic mutation frequency was reported in the eye-colour spot test with Drosophila melanogaster exposed to di(2-ethylhexyl) phthalate in the feed but no effect was observed in two independent wing-spot tests. In a single study, mitotic recombination was not induced by di(2-ethylhexyl) phthalate neither was sex-linked recessive lethal mutation induced in two studies in D. melanogaster treated with di(2-ethylhexyl) phthalate in the feed or by injection. When administered to D. melanogaster, di(2-ethylhexyl) phthalate and A-nitrosodiethylamine induced DNA double-strand breakage and DNA repair, although neither compound was active when administered alone. 

Drosophila melanogaster, loss of heterozygosity by mitotic recombination + 21.8 p.g/mL in feed Vogel Nivard (1993)... 

Although it is necessary to have both alleles altered to give complete loss of function, it is possible to have an intermediate position. If the second allele is altered, then loss of heterozygosity has occurred. This could be through chromosome non-disjunction or mitotic recombination. 

---