Bioreactor disposable

Wave Bioreactor Disposable, sterile, plastic bag bioreactor that is mounted on a rocking platform, which creates wave action inside the bs to mix the culture that grows inside it. 

Wave bioreactor Disposable system, reduces contamination issues and the need for sterilization of the bioreactor Suitable for GMP operations Easily scalable Sampling, monitoring, and control are not as simple as with other systems High cost... 

Product extraction Effluent and waste disposal Medium preparation Seed vessel Purification Cell free supernatant Cell biomass Production bioreactor Downstream processing Medium sterilisation Primary culture Upstream processing... 

Bioreactor landfill operations should comply with RCRA landfill facility standards under 40 CFR Part 264. It should be noted that SARA strongly recommends on-site treatment that permanently and significantly reduces the volume, toxicity, or mobility of hazardous substances, and utilizes cost-effective permanent solutions. The legislation prohibits land disposal of hazardous wastes unless U.S. EPA determines otherwise. U.S. EPA guidance for CERCLA responses requires most on-site disposal actions to attain or exceed applicable and relevant standards of all Federal public health and environmental laws unless specific circumstances dictate otherwise. 

Tobacco cell culture Anti-rabies virus monoclonal antibodies Girard et al., 2006 0.5 mg/L Disposable plastic bioreactor... 

The MiniPERM bioreactor is our method of choice for the high-density culture of hybridoma cells (Fig. 1). The modular system, consisting of a 40-mL disposable culture chamber and a 550-mL reusable nutrient module separated by a dialysis membrane (12.5 kDa molecular weight cutoff), allows for the production of a low-volume, high-density cell population with a correspondingly high antibody yield. 

In general, the subsequent stages occur in small bioreactors, known simply as flasks or bottles, since they have no monitoring and control system and are usually disposable. Apart from flasks and bottles, multiwell plates can be used to culture cells, but their use is restricted mainly to cloning and selection of cells. 

This type of bioreactor consists of two main parts a disposable sterile bag made of flexible plastic, in which cells are cultivated, and a moving platform that supports and shakes the plastic bag. A hydrophobic filter attached to the bag allows the supply of gases, and small tubing allows culture sampling, as well as nutrient addition and product harvesting. 

The plastic bag is disposable and is provided sterile by the manufacturer. This makes it a very attractive bioreactor type for production purposes, especially if the production scale is not very large. Since it is not sterilized in-house, there is no need for validation of the sterilization procedure. The validation of bag sterilization is the responsibility of its manufacturer. Since it is not reused, there is no need for cleaning and validation of cleaning procedures. Figure 9.6 (see color section) shows a photograph and a schematic representation of a wave bioreactor. 

Singh V (1999), Disposable bioreactor for cell culture using wave-induced agitation, Cytotechnology 30 149-158. 

Ventrex (Appendix 3) market a disposable cell lift system which takes up to 6S0 ml suspension. It can be used in batch or continuous feed and a heating jacket is available which means it does not need to be operated in a hot room. Kontes (Appendix 3) sell a similar, reusable Cytolift bioreactor. 

One of the major drawbacks of the stirred-tank bioreactors is the difficulty of cleaning and sterilizing the vessels. Disposable bioreactors are now commercially available that... 

The treatment and safe disposal of waste is equally important whether it be waste generated by the pulp and paper industry, by the leather industry or gaseous waste. Recently, in addition to other membrane-based processes, demand has grown in the wastewater treatment industry for a process that uses both a biological stage and a membrane module, known as the membrane bioreactor (MBR) process. The bioreactor and membrane module each have a specific function (1) the biological degradation... 

The yield of secondary metabolites in a large-scale fermenter typically ranges from 0.1 to lOg/L of broth. Such poor yield leads to cumbersome and expensive processes for both product separation and broth disposal. Within the last decade, several novel bioreactors have been developed for the intensification of fermentation processes. Examples include a centrifugal bioreactor, a rotating packed bed fermenter, and a sonobioreactor. Most of these, however, are yet to be implemented on a production scale because they generally lack practicality and well-defined scale-up criteria. 

In the core disposal operations that follow disassembly, the chemical agents are destroyed by hydrolysis, which is in turn followed by a secondary biotreatment process in immobilized cell bioreactors (ICBs) to treat the streams resulting from the hydrolysis (the hydrolysates) to meet Chemical Weapons Convention requirements and produce environmentally acceptable wastes. 

The anticipated closure wastes are summarized in Table 4-6. These are estimates based on the wastes generated during closure of the Aberdeen Chemical Agent Disposal Facility, which used the same neutralization process to destroy HD mustard agent stored in bulk. Estimates were made of the additional waste from the closure of the bioreactors, which were not a part of the process at Aberdeen, where the hydrolysate was sent to a commercial TSDF. 

Oiled debris, beach material, and sorbents are sometimes disposed of at landfill sites. Legislation requires that this material not contain free oil that could migrate from the site and contaminate groundwater. Some governments have standard leach-ability test procedures that determine whether the material will release oil. Several stabilization processes have been developed to ensure that free oil does not contaminate soil or groundwater. One process uses quick lime (calcium oxide) to form a cement-like material, which can be used on roads as a dust-inhibitor. Another form of disposal is to process liquid oil in a bioreactor and thus attempt to break it down. This is usually not successful because of the many slowly degraded components in some oils. 

In the ANS, the mustard agent is hydrolyzed with hot water and the hydrolysate pH is adjusted with caustic solution. The PCAPP design incorporates six immobilized cell bioreactors (ICBs) for the treatment of agent hydrolysate, although PMACWA continues to investigate off-site shipment options. The water stream from biotreatment is recycled, and the biosludge is sent to an off-site permitted disposal facility. 

For both the General Atomics and the Parsons/Honeywell design packages, the primary treatment to destroy the agent and the energetic materials is hydrolysis. However, the hydrolysis products (hydrolysates) must be further treated before the final products can be properly disposed of. For this secondary step. General Atomics proposes to use supercritical water oxidation (SCWO) and Parsons/ Honeywell proposes to use biotreatment via immobilized cell bioreactors (ICBs). 

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