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Multiwell plates

HTS is usually carried out it multiwell plates and the industry has settled on certain standard formats. One key standard is that most screening assays are carried out in 96 (8 12) or 384 (16 24) well plates with a standard footprint. This standard is important because most automation is optimized for use with these plates and attendant assay volumes in the 100 pL range. Furthermore, source plates containing test compound are typically stored in a similar configuration, allowing for more efficient transfer of test compound from the source plate to the assay plate. Various types of plates are available to match different assay formats, including... [Pg.585]

The advent of automation techniques moved high-throughput ADME screening from individual test tube to multiwell plates. The use of 96- and 384-well plates produced a data explosion and the need to capture, store, and mine data so that it can be used effectively. A database for storing and... [Pg.233]

Miniaturization Currently, permeability studies using cell mono-layers are conducted in automation-friendly 12- or 24-well multiwell plates. Unfortunately, further miniaturization (96- or 384-well cell models) has been technically difficult to achieve because of the small surface area of the... [Pg.198]

Enzyme-linked immunosorbent assay (ELISA) is based on the specific reaction between an antibody and an antigen. One of the reagents in the reaction is labeled with an enzyme that generates a colorimetric product that can be measured with a spectrophotometric device. The color intensity correlates with the concentration of specific antibody and the respective antigen. The reaction can be formatted in various ways in a multiwell plate (microtiter plate) with the common formats being the sandwich assay, the competitive assay, and the direct assay. (See Figure 11.1.)... [Pg.279]

Accuracy also depends on the error of measurement. During assay development, it should be demonstrated that there are no plate edge effects, that is, the response is the same in every well of the multiwell plate. The adsorbing... [Pg.296]

All fluorescence intensity measurements described here were performed using a Perkin-Elmer LS-50B luminescence spectrometer. Some of the methods were adapted to much smaller volumes using 96-well plates and the Bio-Tek Synergy HT multiwell plate reader (equipped with KC-4 software) (Bio-Tek Instruments, Winoaski, Vermont, U.S.A.). [Pg.21]

Multiwell plate reader to measure color at 405 10 nm wavelength. [Pg.236]

Raman microspectroscopy is readily performed on multiple locations inside each well. As in other instances, the results might not be representative of the whole sample because of the small sample volume probed. Polarization effects can be pronounced, but may be mitigated by averaging the results from additional locations. An alternative is rotating the sample, but this usually is not practical for multiwell plates. Both options increase analysis time. Such problems appear to be minimized when handling bulk powders [222,223,230], Several vendors sell systems preconfigured for automated analysis of microtiter plates and are typically integrated with optical microscopy. [Pg.225]

If the chemistry is amenable, it is possible to synthesize a large number of small samples of polymers by simply mixing the ingredients in either small vials or multiwell plates. For example, Brocchini et al. prepared a library of 112 polymers by mixing the monomers in individual vials which were placed in a water bath [22]. Akinc et al. synthesized a library of 24 unique poly(j3-amino esters) via the conjugate addition of acrylates and amines by mixing the monomers in sample vials fitted with stir bars [23]. To speed up this process, a liquid handling robot can be used to dispense the raw materials into an array of vials [24]. [Pg.6]

Transfer each selected embryo to a single well of a multiwell tissue culmre plate (see Note 8) filled with embryo medium containing the substance to be tested (see Note 9). In most cases, the test substance will be evaluated at several concentrations along with a vehicle and/or untreated control, as appropriate. If possible, just prior to transferring embryos pre-fill the multiwell plates with the appropriate medium and test solution... [Pg.393]

In addition to selecting an appropriate assay, it is also necessary to have a pooling strategy. It is more efficient to test many compounds per well on the microplate, rather than one. If one could test 100 compounds per well, then the standard 96-well plate would enable almost 10,000 compounds to be evaluated in one experiment. (Currently, multiwell plates containing more than 96 wells are routinely being used.) To facilitate effective pooling, the library of compounds is usually divided into a number of nonoverlapping subsets. [Pg.125]

Protein-specific arrays allow scientists to carry out parallel studies dealing with identification of protein-protein interactions and the influence of drugs, other chemical entities, and diseases on these interactions. Arrays printed on glass slides or multiwell plates are used to investigate protein-protein and protein-drug interactions. Subsequent analysis is conducted by mass spectrometry tools. [Pg.131]

Materials commonly use as solid supports include polystyrene, polyvinyl, nylon, glass, nitrocellulose, silica, polyacrylamide, or polystyrene beads. Separation of the bound from the free reagents can be achieved through either filtration for particulate solid supports such as agarose, polyacrylamide, and polystyrene beads, or centrifugation. For disposable forms of solid supports such as multiwell plates, plastic tubes, cuvettes, balls, and dipsticks, separation can be performed through simple rinsing steps. [Pg.692]

RIA Using Antigens Bound Directly to PVC Multiwell Plates... [Pg.31]

Transfer 50-pL aliquots of the mixtures to the antibody or antigen-coated PVC multiwell plate so that each well contains 1-4 x 104 cpm of radiolabel. [Pg.32]

Adsorb the antibody to a solid support such as the surface of a plastic tube, multiwell plate, latex particle, magnetic particle, nylon, nitrocellulose, or glass fiber filter. [Pg.357]

Dianilino-l,T-binaphthyl-5,5 -disulfonate (bis-ANS) is a probe that has been shown to increase in fluorescence with soluble Ap in acidic buffer solutions.24 Briefly, Ap 42 was incubated for 30 minutes at room temperature in the presence of different polyphenols. Bis-ANS fluorescence (excitation = 360 nm, emission = 485 nm) was then measured by dilution of 100-pl aliquots in a final volume of 300 pi of citrate buffer (30 mM, pH 2.4) containing bis-ANS (25 pM), using a fluorescence multiwell plate reader (Bio-Tek Instruments , Inc.). To determine amyloid fibril formation, the thioflavin T (Th-T) fluorescence method was performed as previously described.24 Briefly, a fresh solution of Ap 42 was incubated at 37°C for 24 hours in phosphate-buffered saline (pH 7.4). After incubation, a 100-pl aliquot of solution was added in a final volume of 300 pi of phosphate buffer (50 mM, pH 6.0) containing 5 pM Th-T in the presence of different drugs. Fluorescence was then monitored at excitation and emission wavelengths of 450 and 485 nm, respectively. [Pg.109]

SPRI plate. The multiwelled plates move through vacuum manifolds, shakers, heating blocks, and magnetic plate shelf. Gripper arms and conveyer belts are used as necessary. [Pg.356]

The use of online SPE in the column-switching mode is particularly adapted to illicit drug analyses, on the other hand the offline SPE on multiwell plates is qualified for multianalyte procedures since each sample is independently extracted, this format is compatible with different separation techniques working with different mechanisms (reversed or normal phase, ion exchange, etc.) reduces contamination risk presents a great advantage in analyses where a legal aspect has to be taken into account it does not require a particular technical skill [62],... [Pg.383]

In general, the subsequent stages occur in small bioreactors, known simply as flasks or bottles, since they have no monitoring and control system and are usually disposable. Apart from flasks and bottles, multiwell plates can be used to culture cells, but their use is restricted mainly to cloning and selection of cells. [Pg.222]

An in vitro bioassay can be designed in several ways, but requires statistical validity. A one point assay is not valid. The bioassay should be designed to consider factors that introduce variability, and the analysis should test such variability. A measurement series of a test sample should be compared to an equivalent series of the reference material, carefully considering the comparisons between the linear portions of the dose-response curves (Mire-Sluis et al., 1996). To test validity of a bioassay inter- and intra-assay variability should be considered in both preparation, and in the case of multiwell plates, the variability between each plate. To reduce the positional effect in plate tests, it is advisable to distribute the points on the curves randomly and also to include a reference standard in each plate (Gaines-Das and Meager, 1995). One of the most widely used techniques to validate a bioassay s performance is to include internal duplicates. The data arising from the comparison can be important in assessing the test s variability. [Pg.344]

The clones may be isolated either by surrounding each colony with a stainless steel cylinder and removing the cells by trypsin action or by initially seeding the cells into a multiwell plate and selecting those wells in which single colonies become established. If care is taken, single cells can be selected from a suspension and transferred to individual wells in a multiwell plate. [Pg.118]


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