Mouse L cells

Murine macrophage colony-stimulating factor obtained from mouse L cells can be purified more efficiently on a large scale by HPLC in the presence of sodium dodecyl sulfate. The adsorption on hydroxylapatite gel is dependent on the presence of sodium dodecyl sulfate [197]. 

Chu, Y-W., Runyan, R.B., Oshima, R.G., Hendrix, M.J.C. (1993). Expression of complete keratin filaments in mouse L cells augments cell migration and invasion. Proc. Natl. Acad. Sci. USA 90, 4261-4265. 

Dubois, M.F., Mezger, V., Morange, M., Ferrieux, C., Lebon, P., Bensaude, O. (1988). Regulation of the heat-shock response by interferon in mouse L cells. J. Cell Physiol. 137, 102—109. 

Historically, the development of animal cell culture systems has been dependent upon the development of new types of tissue culture media. Mouse L cells and HeLa cells were developed using a balanced salt solution supplemented with blood plasma, an embryonic tissue extract, and/or serum. In 1955 Eagle developed a nutritionally defined medium, containing all of the essential amino acids, vitamins, cofactors, carbohydrates, salts, and small amounts of dialyzed serum (Table 1). He demonstrated that this minimal essential medium (MEM) supported the long-term growth of mouse L and HeLa ceils. Eagle s MEM was so well defined that the omission of a single essential nutrient eventually resulted in the death of these animal cells in culture. 

Pinnaduwage, P., Schmitt, L., and Huang, L., Use of a quaternary ammonium detergent in liposome mediated DNA transfection of mouse L-cells, Biochimica et Biophysica Acta, 1989, 985, 33-37. 

LMTK thymidine kinase-deficient mouse L cells (fibroblasts)... 

I. Transfection of Genes Encoding Proteases Enhances Metastasis. Transfection of different cell types with the genes for either uPA or CD confers or enhances the metastatic ability of the recipient cells. This has been shown for uPA in mouse L cells, ras-transformed NIH 3T3 cells, and murine B16 mela-... 

Cl. Cajot, J. F., Schleuning, W. D., Medcalf, R. L., Bamat, J., Tetuz, J., Libermann, L., and Sordat, B., Mouse L-cells expressing human prourokinase-type plasminogen activator Effects on extracellular matrix degradation and invasion. J. Cell. Biol. 109, 915-925 (1989). 

The following cell cultures and virus have shown to be suitable MDBK cells (ATCC No. CCL22), or Mouse L cells (NCTC clone 929 ATCC No. CCL I) as the cell culture and vesicular stomatitis virus VSV Indiana strain (ATCC No. VR-158) as the infective agent or human diploid fibroblast FS-71 cells responsive to interferon as the cell culture, and encephalomyocarditis virus (ATCC No. VR-129B) as the infective agent. 

Bredinin, Neosidomycin, and SF-2140. Bredinin (CiyH NjO, ) isolated from the culture filtrates of Eupenicillium brefeldianum, inhibits the multiplication of L5I78Y, HeLa 83, RK-13, mouse L-cells, and Chmese hamster cells. 

For Weight Gain in I For Positive Nitrogen For Mouse L Cells... 

Color Plate 9 Laser scanning 3-dimensional confocal images of mouse L-cells mixed with 185... 

Color Plate 9 Laser scannin" -dimensional confocal images of mouse L-cells mixed with positively charged CL/ DNA complexes with theLa structure (50%DOPC-50%DOTAP-Z>gal DNA) and fixed one hour after the transfection. Side views looking inside cell, (see page 205)... 

Lapie P, Goudet C, Nargeot J, Fontaine B, Lory P (1996) Electrophysiological properties of the hypokalaemic periodic paralysis mutation (R528H) of the skeletal muscle alpha Is subunit as expressed in mouse L cells. FEBS Lett 382 244-248. 

Fig. 15 Total gene knockdown (KT) with siRNA complexes targeting the luciferase mRNA in transfected mouse L-cells as a function of mole fraction of neutral lipid at pchg = 15 (a) and pchg = 2.8 (b). Reprinted with permission from [79]. Copyright 2007 American Chemical Society...

Fig. 10.7. Fraction of labelled mitoses. The solid line represents the theoretical change in the fraction of mitotic cells after labelling for 100 min with tritiated thymidine. The dotted line follows data obtained with mouse L cells. The hatched areas represent the duration of the exposure to tritiated thymidine. (Reproduced from Cleaver, 1967, with kind permission of the author.)...

By measuring the incorporation of thymidine into DNA from [3H]thymidine supplied at different concentrations to mouse L cells, Cleaver (1967) was able to show that, at about 10-5M thymidine, incorporation reached a plateau, and a similar observation has been made for CHO cells (Fig. 12.3). This has been interpreted as showing that at this concentration the contribution of endogenous dTTP to DNA thymine is negligible. Care must be taken, however, that at... 

Lucia-Jandris P, Hooper JW, Fields BN. 1993. Reovirus M2 gene is associated with chromium release from mouse L cells. J Virol 67(9) 5339-5345. 

The upper-left panel of figure 6.3 shows how variations in the concentrations of KC1 or K-acetate affect the incorporation of radiolabeled lysine into newly synthesized proteins in mouse L-cells (Weber et al., 1977). Both potassium salts stimulate protein synthesis up to a certain salt concentration, with the acetate salt having a higher optimal concentration and higher final degree of stimulation than KC1. Above the opti-... 

A His residue is often found at the active site of enzymes where it functions as a catalyst in acid-base and nucleophilic processes. Substitution of fluorine on His reduces the pKa by about 5 pH units, and this dramatic drop in basicity is reflected in altered biological properties of FHis-containing proteins. The presence of 2-FHis in cell cultures inhibits the stimulation of several enzymes, for example, the stimulation of pineal gland A-acetyltransferase activity, in cell culture and in vivo. This stimulation is accompanied by His and cyclohex-imide-sensitive incorporation of 2-FHis into cellular protein192,193. A direct comparison of His and 2-F-His in mouse L cells showed that the analogue is incorporated at about 17% the efficiency of the parent194.4-FHis showed none of the above biological activity. 

Estacion, M., Nguyen, H.B., and Gargus, J. J. 1996. Calcium is permeable through a maitotoxin-activated nonselective cation channel in mouse L cells. Am J Physiol 270, Cl 145-Cl 152. 

Hooper, J. W., and Fields, B. N. (1996). Monoclonal antibodies to reovirus crl and fil proteins inhibit chromium release from mouse L cells. /. Virol. 70, 72- T1. 

The method of biosynthetic incorporation of spin label, rather than mechanical addition to isolated material, is a convenient way of ensuring that the results obtained are biologically meaningful and has also been used with such systems as the mould Neurospora crassa [158], Mycoplasma laidlawii [159], human leucocytes, and mouse L cells [160]. The spectra from these two mammalian cells showed distinct similarities for a variety of spin labels, but different spectra were obtained when the labels were incorporated in human erythrocytes. Fractionation of the cell components showed the stearic acid (C, n = 3) spin label in all the major fractions, but by far the largest concentration was in the nuclear membrane. The ESR spectrum underwent a time and temperature dependent decay and spin labels on the surface membrane were reactivated with K3Fe(CN)6. 

Miyagawa S. 1977. Differential effects of continuous and short time treatment with 2,4-dinitrophenol on the cell cycle of mouse L cells. Tokushima J Exp Med 24 147-154. 

Tsuda S. 1974. Effects of 2,4-dinitrophenol, sodium arsenate, and oligomycin on mitosis of mouse L cells growing in monolayer culture. Tokushima J Exp Med 21 49-59. 

Bredinin, Neosidomycin, and SF-2140. Bredinin (62), isolated from the culture filtrates of Eupenicillium brefeldianum (1,4), inhibits the multiplication of L5178Y, HeLa S3, RK-13, mouse L-cells, and Chinese hamster cells. GMP can reverse the inhibition by (62), but (62) is not incorporated into the nucleic acids. The inhibition of nucleic acid synthesis and chromosomal damage in the S and G 2 phases that is caused by (62), is reversed by GMP. It blocks the conversion of IMP to XMP and XMP to GMP. In combination with GMP, (62) interferes with intracellular cAMP levels and thereby inhibits cell division. 

Such repressor molecules have been proposed in other work [90] where transcription of a human -yl gene transfected into mouse L cells appeared to be strongly stimulated by treatment of the cells with the protein synthesis inhibitor cycloheximide. 

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