MDBK cells

The following cell cultures and virus have shown to be suitable MDBK cells (ATCC No. CCL22), or Mouse L cells (NCTC clone 929 ATCC No. CCL I) as the cell culture and vesicular stomatitis virus VSV Indiana strain (ATCC No. VR-158) as the infective agent or human diploid fibroblast FS-71 cells responsive to interferon as the cell culture, and encephalomyocarditis virus (ATCC No. VR-129B) as the infective agent. 

As indicated above, these data do not tell us whether a function" is lacking in MDBK which is essential for viral protein or RNA synthesis, or whether a macromolecule is synthesized which prevents the translation of viral proteins or viral RNA synthesis. Since MDBK can be infected productively by other viruses (Bovine enterovirus, influenza), it must be a molecule specific for the mengo-MDBK cell interaction. The greatest enigma in our data is the origin of the early ENA replicase, since very little (or no) protein synthesis occurs. 

In order to determine whether restriction is due to a lack of host factor as opposed to inhibition by a host factor of viral functions, it was decided to hybridize MDBK with L-cells. We have introduced an HGPRT mutation into the MDBK cells, and utilized a TK strain of L-cells. Hybrids were selected in HAT medium (hypoxanthine, aminopterin, thymidine), clones were selected, grown up, and tested for virus yield. Table 2 summarizes the data obtained. 

Bovine viral diarrhea virus MDBK cells Lecot et al. (2005)... 

Cacan, R., C. Dengremont, O. Labiau, D. Kmiecik, A. M. Mir, and A. Verbert, Occurrence of a cytosolic neutral chitobiase activity involved in oligomannoside degradation a study with Madin-Darby bovine kidney (MDBK) cells, Biochem. J., 1996, 313, 597-602. 

Schulman, J. L., and Palese, P., 1977, Virulence factors of influenza A viruses WSN virus neuraminidase required for plaque production in MDBK cells, J. Virol. 24 170-176. 

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