Neurospora crassa

The use of mutant 34486 of Neurospora crassa for the microbiological assay of ch oline has been described (8). A physiological method has also been used in which the ch oline is extracted after hydrolysis from a sample of biological material and acetylated. The acetylcholine is then assayed by a kymographic procedure, in which its effect in causing contraction of a piece of isolated rabbit intestine is measured (33). 

Nerve growth factor snake venoms zinc, 6, 613 Neurospora crassa calcium transport, 6, 571 cation transport, 6, 559 Neurosporin, 6, 676 Neurotransmitters secretion calcium, 6, 595 Neutral complexes electrical properties, 6, 143 Neutron absorbers... 

The molybdenum cofactor was liberated from D. gigas AOR, and under appropriate conditions was transferred quantitatively to nitrate reductase in extracts of Neurospora crassa nit-1 mutant) to yield active nitrate reductase 217). On the basis of molybdenum content, the activity observed for reconstitution with molybdenum cofactor of D. gigas was lower (25%) than the values observed for the procedure using extractable molybdenum cofactor of XO, used as reference. This result can now be put in the context of the difference in pterin present (MPT-XO and MCD-AOR) 218). 

Neurospora crassa is a filamentous pink mould. It became famous to scientists due to the work of Beadle and Tatum in the 1940s when they developed the one gene—one enzyme hypothesis. Its life cycle is shown in Fig. 2.9. Unforturrately, the full force of fungal nomenclature comes into play when considering this organism. Aerial hyphae... 

E. Physical Methods and Analytical Techniques.—Nucleotide maps of enzymic digests of DNA have been obtained using the same ionophoretic techniques as have been developed for RNA digests. Pancreatic DNase and Neurospora crassa endonuclease produce very similar maps with E. coli DNA but this technique still awaits the discovery of specific DNases. 

Goldie, A.H. and Subden, R.E., The neutral carotenoids of wild-type and mutant strains of Neurospora crassa, Biochem. Genet. 10, 275, 1973. 

Hausmann, A. and Sandmann, G., A single five-step desaturase is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene in Neurospora crassa, Eungal Genet. Biol. 30, 147, 2000. 

Carotene Yellow-orange Neurospora crassa (fungus) RP... 

Purification and characterisation of galactose-induced pectinases from the exo-1 mutant strain of Neurospora crassa... 

Gorlatova N, M Tchorzewski, T Kurihara, K Soda, N Esaki (1998) Purification, characterization, and mechanism of a flavin mononucleotide-dependent 2-nitropropane dioxygenase from Neurospora crassa. Appl Environ Microbiol 64 1029-1033. 

Although the pathway has not been established, relatively high yields of trimethyltin from inorganic tin have been observed in yeast concomitant with the degradation of butyltin compounds (Errecalde et al. 1995). Exceptionally, methionine transferase may carry out the methylation of Hg in Neurospora crassa (Landner 1971) and thiopurine methyltransferase the methylation of inorganic Se in Escherichia coli (Ranjard et al. 2003). 

Landner L (1971) Biochemical model for biological methylation of mercury suggested from methylation studies in-vivo with Neurospora crassa. Nature 230 452-454. 

The reduction of aromatic carboxylic acids to the corresponding aldehydes under aerobic conditions is of interest in biotechnology, since the oxidoreductase from Nocardia sp. is able to accept a range of substituted benzoic acids, naphthoic acids, and a few heterocyclic carboxylic acids (Li and Rosazza 1997). The reaction involves formation of an acyl-AMP intermediate by reaction of the carboxylic acid with ATP NADPH then reduces this to the aldehyde (Li and Rosazza 1998 He et al. 2004). A comparable reaction for aromatic carboxylates has been demonstrated in Neurospora crassa (Gross 1972). 

Gross GG (1972) Eormation and reduction of intermediate acyladenylate by aryl-aldehyde. NADP oxidor-eductase from Neurospora crassa. Eur J Biochem 31 585-592. 

In cells of the fungus Neurospora crassa, 5 mM butyrate (pH 5.8) produced a transient hyperpolarization followed by depolarization... 

Effects of Allelochemlcals on ATPases. Several flavonoid compounds inhibit ATPase activity that is associated with mineral absorption. Phloretin and quercetin (100 pM) inhibited the plasma membrane ATPase Isolated from oat roots (33). The naphthoquinone juglone was inhibitory also. However, neither ferulic acid nor salicylic acid inhibited the ATPase. Additional research has shown that even at 10 mM salicylic acid inhibits ATPase activity only 10-15% (49). This lack of activity by salicylic acid was substantiated with the plasma membrane ATPase Isolated from Neurospora crassa (50) however, the flavonols fisetln, morin, myricetin, quercetin, and rutin were inhibitory to the Neurospora ATPase. Flavonoids inhibited the transport ATPases of several animal systems also (51-53). Thus, it appears that flavonoids but not phenolic acids might affect mineral transport by inhibiting ATPase enzymes. 

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