Syrian hamster cells

Weakley BS, Wehh P, James JL. Cytochemistry of the Golgi apparatus in developing ovarian germ cells of the Syrian hamster. Cell TissueRes 1981 220 349-372. 

Russo, I., A. R. Silver, A. P. Cuthbert, D. K. Griffin, D. A. Trott, and R. F. Newbold. 1998. A telomere-independent senescence mechanism is the sole barrier to Syrian hamster cell immortalization. Oncogene 17(26) 3417-26. 

DiPaolo JA, Casto BC. 1979. Quantitative studies of in vitro morphological transformation of Syrian hamster cells by inorganic metal salts. Cancer Res 39 3008-1013. 

Larramendy ML, Popescu NC, DiPaolo JA. 1981. Induction by inorganic metal salts of sister chromatid exchanges and chromosome aberrations in human and Syrian hamster cell strands. Environ Mutagen 3 597-606. 

Kaul, K.L. Novak, R.F. (1987) Inhibition and induction of rabbit liver microsomal cytochrome P-450 by pyridine. J. Pharmacol, exp. Ther., 243, 384-390 Kerckaert, G.A., Brauninger, R., LeBoeuf, R.A. Isfort, R.J. (1996) Use of the Syrian hamster cell transformation assay for carcinogenicity prediction of chemicals currently being tested by the National Toxicology Program in rodent bioassays. Environ. Health Perspect, 104 (Suppl. 5), 1075-1084... 

Cell transformation, SA7/Syrian hamster cells ND + Hatch et al. (1982)... 

Xylene of unspecified grade did not induce morphological transformation in cultured Syrian hamster cells. 

There are also data that indicate that aluminum does not directly interact with DNA in mutagenicity tests. These data come from negative transformation assays in Syrian hamster cells (DiPaolo and Casto 1979), negative rec (recombination repair) assays in Bacillus subtilis (Kanematsu et al. 1980), and negative Ames assays in Salmonella typhimurium (Marzin and Phi 1985). These data are summarized in Table 2-4. 

J.A. DiPaolo et al., Nitration of carcinogenic and noncarcinogenic polycyclic aromatic hydrocarbons results in products able to induce transformation of Syrian hamster cells. Carcinogenesis 4, 357-359 (1983)... 

Burk (1970) showed that Syrian hamster cells (BHK21/C13) failed to grow when transferred to medium containing 0.25% serum and could be maintained in a quiescent state for 8 days or more. On readdition of serum no DNA synthesis occurred for 9 h and mitotic peaks were observed at about 23 and 33 h. It appeared the cells had come to rest in G1 and on stimulation showed a lag of about 9 h before entering into exponential growth with a generation time of about10-12h. 

Mally, M. I., Grayson, D. R., and Evans, D. R. (1980). Catalytic synergy in the multifunctional protein that initiates pyrimidine biosynthesis in Syrian hamster cells. J. Biol Chem., 255, 11372-11380. 

Syrian hamster cells Chromosomal aberrations No data + Lavappa et al. 1975 CH ... 

Hesterberg TW, Butterick CJ, Oshimura M, et al. 1986. Role of phagocytosis in Syrian hamster cell transformation and cytogenetic effects induced by asbestos and short and long glass fibers. Cancer Res 46 5795-5802. 

Oshimura M, Hesterberg TW, Barrett JC. 1986. An early, nonrandom karyotypic change in immortal Syrian hamster cell lines transformed by asbestos Trisomy of chromosome 11. Cancer Genet Cytogenet 22 225-237. 

Transformation of Syrian Hamster Cells by Metals. When cultures of cells were exposed to two nickel compounds with different carcinogenic potencies, there were differences in the incidence of transformed colonies that formed (Table II). Treatment with amorphous NiS resulted in a low incidence of transformation while treatment with crystalline NiaS2 induced the appearance of numerous transformed colonies (Table II). In this series of experiments, control cultures had no transformed colonies however, in some experiments there was an occasional incidence of spontaneous transformation. The induction of transformation by... 

Carcinogenic metal compoimds such as crystalline Ni3S2 induced a dose-dependent statistically significant incidence of transformation in secondary cultures of Syrian hamster cells. A chemically related compound, amorphous NiS, which is not carcinogenic, resulted in no statistically significant incidences of transformed colonies. Untreated control cultures also had no statistically significant incidence of transformation. We have... 

This is a Syrian hamster cell line derived from the kidneys of 1-day-old hamsters. The cells have a fibroblast-like morphology and are used for viral replication studies, including poliovirus, rabies (Pay et al, 1985), rubella, foot and mouth disease virus (Radlett et al, 1985), VSV, HSV, adenovirus (Ad) 25 and arbovirus. Successful cultivation at scales up to 80001 has been achieved with maximum cell density attained by minimum air sparging sufficient to satisfy the oxygen demand of the cells. 

Smith, K, A, Gorman, P. A., Stark, M. B., Groves, R. P., Stark, G. R. (1990). Distinctive chromosomal structures are formed very early in the amplification of CAD genes in Syrian hamster cells. Cell, 63, 1219-1227. 

Mengovirus replicates efficiently in HeLa (human), L (mouse) and BHK (syrian hamster) cells but replicates poorly in rabbit embryo fibroblasts, PtK-1 cells (kangaroo rat), and MBBK cells (25). Restriction of viral growth is the most severe in MBBK cells with viral yields of only 10-20 PFU/cell seen. Yields in permissive L cells range from 5OO-IOOO PFU/cell. 

In this chapter, emphasis will be placed on the use of early passage Syrian hamster cells for identifying carcinogens. Results from this cell transformation system will be correlated with reported results from the bacterial mutagenesis system and chronic carcinogenicity tests in animals. 

Y. Ishii, J. A. Elliott, N. K. Mishra, and M. W. Lieberman, Quantitative studies of transformation by chemical carcinogens and ultraviolet radiation using a subclone of BHK21 Clone 13 Syrian hamster cells. Cancer Res. 37, 2023-2029 (1977). 

All the inferences and speculations presented thus far are based upon observations of the first-discovered interspecific hybrids— those between rat X mouse cells, and between mouse and (Chinese and Syrian) hamster cells. Some more recent observations made on three other hybrid combinations, appear as exceptions to the rule of coordination emphasized in the preceding Sections and may call for a modification of some of the hypotheses formulated above. We shall therefore describe now the peculiar features of these three new types of hybrids (recorded in Table 2) and then briefly consider their possible causes. 

For example, the generation times of the Syrian hamster cells, 3460-3, the mouse cells, N-2-2, and of their hybrid are, respectively, 20, 35, and 60 hoius. An example of the opposite relationship is observed in the intraspecific hybrid cross Py 27-6 and T 6 (diploid senescent cells), isolated by Yoshida and Ephrussi (1967). The generation times of the parents and hybrid are 27-6, 16.5 hours T 6, several days hybrid, 14.5 hours. It will be noted also that many intraspecific hybrids were isolated without recourse to a selective system, i.e., owing to the rapid overgrowth of both parental cell lines by the hybrids. 

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