Immunohistochemical detection

Choi JK, Mauger J, McGowan KL. Immunohistochemical detection of Aspergillus species in pediatric tissue samples. Am. J. Clin. Pathol. 2004 121 18-25. 

Almqvist PM, Mah R, Lendahl U, et al. Immunohistochemical detection of nestin in pediatric brain tumors. J. Histochem. Cytochem. 2002 50 147-158. 

MacGrogan G, Soubeyran I, De Mascarel I, et al. Immunohistochemical detection of progesterone receptors in breast invasive ductal carcinomas. Appl. Immunohistochem. 1996 4 219-227. 

Grizzle WE, Stockard CR, Billings PE. The effects of tissue processing variables other than fixation on histochemical staining and immunohistochemical detection of antigens. J. Histotechnol. 2001 24 213-219. 

Liu H, Shi J, Wilkerson M, et al. Immunohistochemical detection of pl6INK4a in liquid-based cytology specimens on cell block sections. Cancer (Cancer Cytopathol.) 2007 111 74-82. 

Western blot and immunohistochemical detection are the two most widely used techniques to confirm the expression of a given P450 isoform in isolated brain regions or thin slices. Antibodies detect actual protein,... 

Trap C, Nato F, Chalbot S, Kim SB, Lafaye P, et al. 2005. Immunohistochemical detection of the human cytochrome P4507B1 production of a monoclonal antibody after cDNA immunization. J Neuroimmunol 159 41-47. 

Larson et al. (1996) investigated the ability of acute chloroform vapor exposure to produce toxicity and regenerative cell proliferation in the liver of female B6C3F, mice. Groups of 5 animals were exposed to 0, 0.3, 2, 10, 30, or 90 ppm chloroform via inhalation for 6 hours a day for 4 consecutive days. Animals were administered BrdU via an implanted osmotic pump, and cell proliferation was quantitated as the percentage of cells in S-phase (LI) measured by immunohistochemical detection of BrdU-labeled nuclei. 

Krenacs, L., Tiszlavicz, L., Krenacs, T., and Boumsell, F. (1993) Immunohistochemical detection of CDla antigen in formalin-fixed and paraffin-embedded tissue sections with monoclonal antibody OlO. J. Pathol. 171, 99-104. 

Combined MicroRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers... 

Richmond, R.E., DeAngelo, A.B. Daniel. F.B. (1992) Immunohistochemical detection of ras and myc oncogene expression in regenerating rat liver. Toxicol. Lett., 60, 119-129... 

Immunohistochemical Detection of Bromodeoxyuridine-Labeled Nuclei for In Vivo Cell Kinetic Studies... 

Immunohistochemical Detection of Cells in the Division Cycle Using Antibodies to Proliferating Cell Nuclear Antigen (PCNA)... 

The following hydrated autoclave method can be employed for immunohistochemical detection of molecules in both cultured cell and tissue specimens. The method was used, for example, to localize androgen receptor in cultured LNCaP cells (derived from prostatic carcinoma metastasized to lymph node) and biopsy specimens from patients with prostatic carcinoma (Ehara et al 1996). After being removed from the culture medium, the cells on plastic cover slips are fixed with 10% formalin for 10 min at 20°C. Tissue specimens are fixed for 1-2 days and embedded in paraffin. Sections (5 pan) are cut, mounted on glass slides, and heated in an oven for 1 hr at 42°C to promote adherence to the slide. After deparaffmizing and rehydration, the sections are subjected to epitope retrieval treatment as follows. 

Immunohistochemical detection of wild-type and mutant p53 proteins can be carried out in fresh-frozen as well as formalin-fixed and paraffin-embedded tissues. This is best accomplished by using antigen retrieval with microwave heating or other types of heating such as autoclaving. A number of monoclonal antibodies are commercially available, the characteristics and sources of which are listed in Tables 10.3 and 10.4 and on pages 50-51. 

Birner, P., Ritzi, M., Musahl, C., Knippers, R., Geredes, J., Voigtlander, T., Budka, H., and Hainfellner, J. A. 2001. Immunohistochemical detection of cell growth fraction in formalin-fixed and paraffin-embedded murine tissue. Am. J. Pathol. 755 1991-1996. 

Dowell, S. P., and Ogden, J. R. 1996. The use of antigen retrieval for immunohistochemical detection of p53 overexpression in malignant and benign oral mucosa A cautionary note. 7. Oral Pathol. Med. 25 60-64. 

Hiasa, Y., Nishioka, H., Kitahori, Y., Yane, K., Nakoka, S., Ohshima, M., and Sugimura, M. 1991. Immunohistochemical detection of estrogen receptors in paraffin sections of human thyroid tissues. Oncology 48 421 424. 

Hirabayashi, S. 1999. Immunohistochemical detection of DNA topoisomerase type II a and Ki-67 in adenoid cystic carcinoma and pleomorphic adenoma of the salivary gland. 7. Oral Pathol. Med. 25 131—136. 

Janssen, P. J., Brinkmann, A. O., Boersma, W. J., and van der Kwast, T. H. 1994. Immunohistochemical detection of the androgen receptor with monoclonal antibody F39.4 in routinely processed, paraffin-embedded human tissues after microwave pretreatment. J. Histochem. Cytochem. 42 1169-1175. 

Mangham, D. C., and Isaacson, P. G. 1999. A novel immunohistochemical detection system using mirror image complementary antibodies (MICA). Histopathology 35 129-133. 

McCluggage, W. G., Maxwell, P., and Bharucha, H. 1998. Immunohistochemical detection of met-allothionein and MIB1 in uterine cervical squamosa lesions, hit. J. Gynecol. Pathol. 77 29-35. 

Mote, P. A., Leary, J. A., and Clarke, C. L. 1997. Immunohistochemical detection of progesterone receptors in archival breast cancer. Biotech. Histochem. 73 117-127. 

Oyaizu, T., Arita, S., Hatano, T., and Tsubura, A. 1996. Immunohistochemical detection of estrogen and progesterone receptors performed with an antigen-retrieval technique on methacam-fixed paraffin-embedded breast cancer tissues. J. Surg. Res. 60 69-73. 

Piffko, J., Bankfalvi, A., Ofner, D., Joos, U., Bocker, W., and Schmid, K. W. 1995. Immunohistochemical detection ofp53 protein in archival tissues from squamous cell carcinomas of the oral cavity using wet autoclave antigen retrieval. J. Pathol. 776 69-75. 

Wrba, F., Gullick, W. J., Fertl, H. et al. 1989. Immunohistochemical detection of the c-erbB-2 proto-oncogene product in normal, benign and malignant cartilage tissues. Histopathology 75 71-76. 

Table 3). They have been detected both functionally (Table 3) and, morphologically, by comparison of the distribution of H3 receptor mRNA and H3 receptor protein. By the latter approach it has been shown, for example, that thalamo-cortical and hippocampal pyramidal glutamate neurons and striato-nigral as well as striato-pallidal GABA neurons possess presynaptic H3 receptors (see Pillot et al. 2002 Jin and Panula 2005 see also immunohistochemical detection in Section 3.9). 

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