Immunohistochemical

The compatibility has been detected mostly by Lee and coworkers. They confirmed the ability of rat aortic SMC to attach and proliferate on the PGCL surface, and then the seeded scaffolds were implanted subcutaneously in nude mice and evaluated histologically and immunohistochemi-cally. SMCs were proliferated and differentiated immunohistochemically. 

In a histometric, immunohistochemical and ultrastructural study, TCA peeling in concentrations of 10,20 and 30% were compared with dermabrasion in nine dark-skinned patients (Fitzpatrick s IV and V) with photodamage [24]. Both procedures induced increasing amounts of types I and III collagen. However,... 

McManus C, Berman JW, Brett EM, Staunton H, FarreU M, Brosnan CE (1998) MCP-1, MCP-2 and MCP-3 expression in multiple sclerosis lesions an immunohistochemical and in situ hybridization study. J Neuroimmunol 86 20-29... 

Xia M, Qin S, McNamara M, Mackay C, Hyman BT (1997) lnterleukin-8 receptor B immunore-activity in brain and neuritic plaques of Alzheimer s disease. Am J Pathol 150 1267-1274 Xia MQ, Bacskai BJ, Knowles RB, Qin SX, Hyman BT (2000) Expression of the chemokine receptor CXCR3 on neurons and the elevated expression of its ligand IP-10 in reactive astrocytes in vitro ERKl/2 activation and role in Alzheimer s disease. J Neuroimmunol 108 227-235 Xia MQ, Qin SX, Wu LJ, Mackay CR, Hyman BT (1998) Immunohistochemical study of the beta-chemokine receptors CCR3 and CCR5 and their Ugands in normal and Alzheimer s disease brains. Am J Pathol 153 31-37... 

Immunohistochemical studies carried out in our laboratories have demonstrated the presence of xanthine oxidase in synovial endothelial cells (Stevens etal., 1991). As expected, the activity of this enzyme per unit weight of tissue is generally higher in synovia taken from RA patients due to their increased vascularity (Allen et al., 1987). In addition, it has also been shown that rheumatoid synoviocytes contain increased levels of iron-saturated ferritin (Morris et d., 1986). Xanthine oxidase (but not dehydrogenase) is able to mobilize iron from ferritin, supplying the necessary transition metal catalyst for the Haber-Weiss reaction and promoting OH formation (Biemond eta/., 1986). 

In collaboration with PA Baeuerle (Freibuig), active NF-xB has been detected immunohistochemically in rheumatoid synovium by using a polyclonal antibody directed against the Rel-A NLS subunit of NF-xB (M.L. Kus, unpublished observations). The antibody employed in these studies was considered to be activity specific because IxB sterically masks the NLS sequence. NF-xB activation by ROM in the rheumatoid joint may orchestrate some of the chronic inflammatory processes characteristic of this disease. It is plausible that lL-1 and TNFo generated in the inflamed synovium as well as the up-regulated expression of adhesion molecules may be under the influence of NF-xB. 

Injury (either physical or chemical) to the comeal endothelial cells has a marked efiect on occular function as these cells are responsible for maintaining the thickness and clarity of the cornea, yet they cannot be replaced if damaged. Immunohistochemical studies have revealed that enzymatic antioxidant defences, SOD, CAT and GSHPx, are similarly distributed in the corneal epithelium and endothelium (Rao etal., 1985 Attala et d., 1987, 1988). Other antioxidants include ascorbate, carotenoids and vitamin E (Fleath, 1962). 

Oka, S., Ogino, K., Hobara, T., Yoshimura, S., Okazaki, Y., Takemoto, T., Kato, N., lida, Y. and Uda, T. (1990b). An immunohistochemical study of copper, zinc-containing superoxide dismutase detected by a monocloncal antibody in gastric mucosa and gastric cancer. Histopathology 17, 231-236. 

Previous studies have also indicated that fenfluramine produces signs of neuronal degeneration (Harvey and McMaster 1975 Harvey and McMaster 1977 Harvey et al. 1977). Recent immunohistochemical studies also indicated that fenfluramine produced morphological damage to 5-HT terminal fields (Appel and De Souza 1988). Collectively, the neurochemical and histological data support the idea that fenfluramine is neurotoxic to 5-HT. 

Fritschy, J.-M., and Grzanna. R. Immunohistochemical analysis of the neurotoxic effects of DSP-4 identifies two populations of noradrenergic axon terminals. Neuroscience 30 181-198, 1989. 

Kohler, C. Chan-Palay, V. Haglund, L. and Steinbusch, H.W.M. Immunohistochemical localization of serotonin nerve terminals in the lateral entorhinal cortex of the rat Demonstration of two separate patterns of innervation from the midbrain raphe. Anat Embryol 160 121-129, 1980. 

Kohler, C., and Steinbusch. H.W.M. Identification of serotonin and non-serotonin-containing neurons of the mid-brain raphe projecting to the entorhinal area and the hippocampal formation. A combined immunohistochemical and fluorescent retrograde tracing study in the rat brain. Neuroscience 7 951-975. 1982. 

Lidov, H.G.W., and Molliver, M.E. An immunohistochemical study of serotonin neuron development in the rat Ascending pathways and terminals fields. Brain Res Bull 8 389-430, 1982. 

Takahashi S., Iwanaga T Takahashi Y., Nakano Y., et al. (1984). Neuron-specific enolase, neurofilament protein and S-100 protein protein in the olfactory mucosa of human fetuses an immunohistochemical study. Cell Tiss Res 238, 231-234. 

Evidence implicating CX3CR1 and fractalkine in atherosclerosis is growing. Immunohistochemical analysis of human atherosclerotic lesions demonstrate that macrophages in the intima as well as smooth muscle cells, mononuclear cells, and foam cells in the deep intima and media express CX3CL1, whereas normal arteries do not (57,58). 

Kraushaar BS, Nirschl RP. Tendinosis of the elbow (tennis elbow). Clinical features and findings of histological, immunohistochemical, and electron microscopy studies. J Bone Joint Surg Am 1999 81(2) 259-278. 

CEA levels are elevated in benign diseases such as inflammatory bowel disease, chronic gastritis and peptic ulcer, cirrhosis, and hepatitis. CEA testing is recommended primarily to monitor patients after surgery for recurrent colorectal carcinoma. Twenty percent of colorectal carcinomas do not express CEA therefore, immunohistochemical methods are recommended to identify the negative cases. If a 5.0 ng/ml cutoff is used as the detection criterion, approximately 60 to 90 percent of the clinical cases will be detected for recurrences 2 to 10 months prior to clinical symptoms (37, 38). 

Miller RH, David S, Patel R, Abney ER, Raff MC 1985 A quantitative immunohistochemical study of macroglial cell development in the rat optic nerve in vivo evidence for two distinct astrocyte lineages. Dev Biol 111 35-41... 

Noack S, Lilienthal H, Winneke G, et al. 1996. Immunohistochemical localization of neuronal and glial calcium-binding proteins in hippocampus of chronically low level lead exposed Rhesus monkeys. Neurotoxicology 17(3-4) 679-684. 

Chalabi, N, L Delort, S Satih, P Dechelotte, YJ Bignon, and DJ Bemard-Gallon. 2007. Immunohistochemical expression of RARalpha, RARbeta, and Cx43 in breast tumor cell lines after treatment with lycopene and correlation with RT-QPCR. J Histochem Cytochem 55(9) 877-883. 

Studies of sleep-active neuronal discharge across the sleep-wake cycle in freely moving animals provide important information about the hypnogenic process (see below) but, because of sampling limitations, are not suitable for systematic mapping of the exact locations of putative hypnogenic neurons. The application of the c-Fos immunoreactivity (IR) method to map sleep-active neurons has stimulated several advances. C-Fos IR is a marker of neuronal activation in most brain sites immunohistochemically labeled neurons can be mapped systematically. The localization of c-Fos IR following sustained sleep, but not... 

Fort, R, Khateb, A., Pegna, A., Muhlethaler, M. 8r Jones, B. E. (1995). Noradrenergic modulation of cholinergic nucleus basalis neurons demonstrated by in vitro pharmacological and immunohistochemical evidence in the guinea-pig brain. Eur. J. Neurosci. 7, 1502-11. 

Jones, B. E. Beaudet, A. (1987). Distribution of acetylcholine and catecholamine neurons in the cat brainstem a choline acetyltransferase and tyrosine hydroxylase immunohistochemical study. J. Comp. Neurol. 261, 15-32. 

Manns, I. D., Alonso, A. Jones, B. E. (2000). Discharge profiles of juxtacellularly labeled and immunohistochemically identified GABAergic basal forebrain... 

Shiromani, P. J., Armstrong, D. M. Gillin, J. C. (1988). Chohnergic neurons from the dorsolateral pons project to the medial pons a WGA-HRP and choline acetyltransferase immunohistochemical study. Neurosci Lett. 95, 19-23. 

Watanabe, T., Taguchi, Y., Shiosaka, S. et al. (1984). Distribution of the histaminergic neuron system in the central nervous system of rats a fluorescent immunohistochemical analysis with histidine decarboxylase as a marker. Brain Res. 295, 13-25. 

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