Image Complementary Antibodies

The sections are incubated in 1 30 diluted MICA antibody No. 1 (peroxidase-conjugated donkey antisheep Ig) for 20 min and washed with TBS/Tween. They are incubated in 1 30 diluted MICA antibody No. 2 (sheep antidonkey Ig) for 20 min and washed in TBS/Tween. This is followed by incubation with 1 30 diluted MICA antibody No. 1 (peroxidase-conjugated donkey antisheep Ig) for 20 min and washed in TBS/Tween. The sections are exposed to 1 mg/ml DAB-0.02% H202 in TBS for 6 min and washed in TBS. They are dehydrated in ethanol, counterstained with hematoxylin, and cover-slipped. 

All the reagents are commercially available in kit form (po/yMICA Binding Site Ltd., Birmingham, UK). 

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Mangham, D. C., and Isaacson, P. G. 1999. A novel immunohistochemical detection system using mirror image complementary antibodies (MICA). Histopathology 35 129-133. 

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