Cover slips plastic

Continue with Subheading 3., step 18 in the method. The positive reaction product with this chromogen will be red, with the nuclei a light blue. Rather than dehydrating the specimens in ethanol and xylene, allow them to dry, add 1 drop of Crystal/Mount (Biomeda Corp, Foster City, CA) to the specimen and bake them in a 60°C oven for 30 min. This preparation is permanent and can be cover-slipped with Permount if needed. The Crystal/Mount will form a hard plastic coating on the slide, but it can be damaged by smudging. 

Add 100 J L of IX PCR reaction buffer on the top of the each slide, cover the tissue with either a clean plastic or a glass cover slip, and seal with rubber cement (see Notes 18 and 25). 

The following hydrated autoclave method can be employed for immunohistochemical detection of molecules in both cultured cell and tissue specimens. The method was used, for example, to localize androgen receptor in cultured LNCaP cells (derived from prostatic carcinoma metastasized to lymph node) and biopsy specimens from patients with prostatic carcinoma (Ehara et al 1996). After being removed from the culture medium, the cells on plastic cover slips are fixed with 10% formalin for 10 min at 20°C. Tissue specimens are fixed for 1-2 days and embedded in paraffin. Sections (5 pan) are cut, mounted on glass slides, and heated in an oven for 1 hr at 42°C to promote adherence to the slide. After deparaffmizing and rehydration, the sections are subjected to epitope retrieval treatment as follows. 

Place the slides sideways into a glass or plastic staining trough and place this into an easily sealable container (e.g., a Tupperware box with a clip-on lid). Humidify the chamber by placing some tissues soaked in 2X SSC on the bottom. Incubate overnight at 42°C. If the slides are well humidified and level, there is no need to seal the cover slips with rubber cement... 

FIGURE 2.10 An array of commercially available and commonly used plastic plates for both sitting and hanging drop vapor diffusion crystallization. Also in the picture is a box of silicone coated cover slips for hanging drops. Courtesy of Hampton Research. 

Preparatory work cut plastic trays in the required size and siliconise microscope glass cover slips of 22x22 mm. Keep the cover slips in an oven at 110°C during the night. 

Development of a simple technique has been reported for coating glass cover slips and plastic Petri dishes with various immune complexes.The supports were coated with poly(L-Lys), which was then treated with 2,4-dinitrobenzene sulphonate followed by glutaraldehyde-mediated reaction with the appropriate substance. 

In some cases, the SEM substrate can be a TEM grid. More typically, ideal substrates are flat or relatively featureless or both. Pre-cleaned glass or plastic cover slips are commonly used for cell layers and cells that can adhere. Use of pre-cut silicon chips as a substrate is gaining popularity, as these are very flat and featureless and sufficiently conductive to minimize charge buildup in samples. In many cases, cells and bacteria adhere to cover slips and chips without further treatment. If necessary, the substrates can be coated with polypeptides or other agents such as collagen, fibronectin, serum, or polylysine to promote adherence. 

The key question for the cell biologist is whether the softness of such tissue - versus the rigidity of glass cover slips and tissue culture plastic (polystyrene) - influences cell stmcture and function. 

Plastic cover slips pieces of appropriate size cut from polyethylene autoclavable waste disposal bags. 

For steps in the schedule using volumes of 200 oL or less the solution is pipeted onto the slide and covered with a plastic cover slip. All washing steps are carried out in Coplin jars using a sufficient volume of liquid to fully immerse the whole slide. Slides must be handled carefully to avoid scratching. They should never dry out during incubation or between steps (except after step 9 of Section 3.1.). 

Add 40 pL denatured hybridization mixture to each slide and cover with a plastic cover slip. Ensure no bubbles are trapped. Quickly place the slides in the preheated humid chamber and incubate for 10 min at 90°C see Note 8). 

Add 200 jL of BSA block to each slide, cover with a plastic cover slip, and incubate for 5 min at room temperature (see Note 6). 

Remove the cover slip, drain the slide (see Note 7), and add 30 pL of the appropriate antisheep conjugate. Cover with a plastic cover slip and incubate slides in a humid chamber for 1 h at 37°C. 

Fig. 112. Ruffled coat of a peritoneal macrophages from a SPF-NMRl mouse 48 h after a single intraperitoneal injection of 1 ml NaCl. The cells harvested with the ascites were centrifuged for 10 min at 1000 r.p.m. and incubated in TCM 199 + 20 % fetal calf serum on a plastic cover slip for 2 h, rinsed with phosphate buffered saline after Dulbecco and fixed in 1 % glutaraldehyde in 0.07 M phosphate huffer (pH 7.4). Ethanol, amyl acetate. Critical point drying. Gold coating. Cambridge Stereoscan 150 operated at 19 kV. APh-R. 854/80, negative 07 162. (from Schiller 1982)...

Neurons do not attach, mature, or survive properly when grown directly on plastic tissue culture dishes. In addition, immunofluorescence imaging and quantification can only be carried out easily on cells grown on cover-slips. Therefore, we plate hippocampal neurons onto glass coverslips that have been cleaned, sterilized, and coated with poly-l-lysine to improved cell adhesion (Banker, 1991). 

An 80 m3 tank fiberglass-reinforced plastic acid tank was blown apart at the base as the result of overpressure. The vent had been slip-plated so the tank could be entered for inspection. The steel slip-plate was covered with a corrosion-resistant sheet of polytetrafluoroethylene. Afterward, when the slip-plate was removed, the sheet was left behind. This did not matter at the time, as the tank was also vented through an oveiflow line,... 

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