Immunohistochemical assays

Rhodes A, Jasani B, Anderson E, et al. Evaluation of HER-2/neu immunohistochemical assay sensitivity and scoring on formalin-fixed and paraffin-processed cell lines and breast tumors a comparative study involving results from laboratories in 21 countries. Am. J. Clin. Pathol. 2002 118 408 117. [Pg.99]

Although the two methods mentioned above are viable options, they should not be applied independently of each other. A tested, standardized, and well-controlled immunohistochemical assay should serve as the first test. It is simple to carry out and quite reliable for the two extreme ends of the staining spectrum (9 and 3+) (Hendricks, 2000). The 2+ cases (weak staining in at least 10% of neoplastic cells) can in turn be subjected to FISH analysis to confirm the presence of an altered HER-2Ineu gene. [Pg.299]

Battifora, H., Gaffey, M., Esteban, J., Mehta, R, Bailey, A., Faucett, C., and Niland, J. 1991. Immunohistochemical assay of neu/c-erbB-2 oncogene product in paraffin-embedded tissues in early breast cancer Retrospective follow-up study of 245 stage I and II cases. Mod. Pathol. 4 466-414. [Pg.307]

Esteban, J. M., Ahn, C., Battifora, H., and Felder, B. 1994a. Quantitative immunohistochemical assay for hormonal receptors Technical aspects and biological significance. J. Cell Biochem. 79 138-145. [Pg.315]

Huang, A., Pettigrew, N. M., and Watson, P. H. 1996. Immunohistochemical assay for oestrogen receptors in paraffin wax sections of breast carcinoma using a new monoclonal antibody. J. Pathol. 180.223-221. [Pg.322]

Maia, D. M. 1999. Immunohistochemical assay for HER2 overexpression. J. Clin Oncol. 77 1650. [Pg.329]

Dybdal N, Leiberman G, Anderson S, et al. Determination of HER2 gene amplification by fluorescence in situ hybridization and concordance with the clinical trials immunohistochemical assay in women with metastatic breast cancer evaluated for treatment with trastuzumab. Breast Cancer Res. Treat. 2005 93 3-11. [Pg.111]

Nichols GE, Frierson HF, Boyd JC, Hanigan MH. Automated immunohistochemical assay for estrogen receptor status in breast cancer using monoclonal antibody CC4-5 on the Ventana ES. Am J CHn Pathol 1996 106 332-38. [Pg.791]

The sensitivity of IHC is better than light microscopic identification of viral inclusions and compares favorably with culture and in situ hybridization. Additionally, immunohistochemical assays can be completed faster than the shell vial culture technique, allowing for rapid results that are important for early anti-CMV therapy. ... [Pg.61]

Recently IHC has been successfully used to identify Streptococcus pneumoniae in formalin-fixed organs with an overall sensitivity of 100% and a specificity of 71% when compared with cultures.Immunohistochemical assays are used to identify Clostridium sp., S. aureus, and S. pyogenes Haemophilus influenzae Chlamydia species i i Legionella pneumophila and L. dumoffiif Listeria monocytogenesf Salmonellaand rickettsial infections other than Rocky Mountain spotted fever such as boutonneuse fever, epidemic typhus, murine typhus,rickettsialpox, 489 African tick bite fever,i and scrub typhus. [Pg.67]

Immunohistochemical assays using polyclonal antibodies specific for Balamuthia mandrillaris, Naegleria fowleri, and Acanthamoeba sp. are used to demonstrate amebic trophozoites and cysts in areas of necrosis and can allow their differentiation from macrophages in cases of amebic meningoencephalitis. ... [Pg.69]

Zaki SR, Shieh W-J, Greer PW, etal. A novel immunohistochemical assay for the detection of Ebola virus in skin implications for diagnosis, spread, and strrveillance of Ebola hemorrhagic iever. J Infect Dis. 1999 179(suppl 1) S36-S37. [Pg.76]

Torres-Velez FJ, Nace EK, Won KY, et al. Development of an immunohistochemical assay for the detection of babesiosis in formalin-fixed, paraffin-embedded tissue samples. Am J Clin Pathol. 2003 120 833-838. [Pg.80]

Genrich GL, Guarner J, Paddock CD, et al. Fatal malaria infection in travelers Novel immunohistochemical assays for the detection of Plasmodium falciparum in tissues and implications for pathogenesis. Am J Trap Med Hyg. 2007 76 251-259. [Pg.80]

Argani P, Lai P, Hutchinson B, et al. Aberrant nuclear immu-noreactivity for TFE3 in neoplasms with TFE3 gene fusions A sensitive and specific immunohistochemical assay. Am J Surg Pathol. 2003 27 750-761. [Pg.130]

Immunohistochemical studies can give information on the formation and repair of adducts in single cells as well as clarifying the distribution and localization of adducts over different cell types in tissue sections. For immunohistochemical assays, however, it is necessary to denature DNA/or remove chromosomal proteins in order to allow the immune reagents access. Therefore optimal conditions for the acid treatment must be studied carefully, to minimize adduct instability. It remains to be established whether the presently applied methods are suitable for accurate quantification of adducts in cells.(6-8, 21)... [Pg.254]

Kennedy AS, Raleigh JA, Varia MA (1997) Proliferation and hypoxia in human squamous cell carcinoma of the cervix first report of combined immunohistochemical assays. Int J Radiat Oncol Biol Phys 37 897-905... [Pg.9]

Antibody-based methods have also been used, e.g. immunohistochemistry and electron immunohistochemical assays on parasitic infections in molluscs (e.g. Anderson et al, 1994 Boulo et al, 1989 Carnegie and Cochennec-Laureau, 2004 Dungan and Roberson, 1993 Mialhe et al, 1988). Clearly... [Pg.132]

ANDERSON T J, MCCAUL T F, BOULO V, ROBLEDO J A F and LESTER R J G (1994), Light and electron immunohistochemical assays on paramyxean parasites, Aquat Living Resour, 1,47-52. [Pg.142]

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