Immunohistochemical analysis

Fritschy, J.-M., and Grzanna. R. Immunohistochemical analysis of the neurotoxic effects of DSP-4 identifies two populations of noradrenergic axon terminals. Neuroscience 30 181-198, 1989. 

Evidence implicating CX3CR1 and fractalkine in atherosclerosis is growing. Immunohistochemical analysis of human atherosclerotic lesions demonstrate that macrophages in the intima as well as smooth muscle cells, mononuclear cells, and foam cells in the deep intima and media express CX3CL1, whereas normal arteries do not (57,58). 

Watanabe, T., Taguchi, Y., Shiosaka, S. et al. (1984). Distribution of the histaminergic neuron system in the central nervous system of rats a fluorescent immunohistochemical analysis with histidine decarboxylase as a marker. Brain Res. 295, 13-25. 

It is well accepted that MDMA produces 5-HT depletions in rat CNS, but much less attention has been devoted to the effects of MDMA on established markers of neurotoxicity such as cell death, silver-positive staining, and reactive gliosis. Support for the hypothesis of MDMA-induced axotomy relies heavily on immunohistochemical analysis of 5-HT levels, which could produce misleading results if not validated by other methods. For example, MDMA-induced loss of 5-HT could be due to persistent adaptive changes in gene expression or protein function, reflecting a state of metabolic quiescence rather than neurotoxic damage. Table 7.3 summarizes the effects of MDMA on hallmark measures of neurotoxicity. 

OATP-C, a liver-specific member of this transporter family, was cloned by number of groups [17, 30-32] and often referred by aliases such as liver-specific transporter 1 (LST-1) and OATP2. Immunohistochemical analysis proved OATP-C expression on the basolateral membrane of hepatocytes [32]. As with OATP-A, OATP-C... 

Kelly D. and King T.P. (1991). The influence of lactation products on the temporal expression of histo-blood group antigens in the intestines of suckling pigs lectin histochemical and immunohistochemical analysis . Histochem J, 23, 55-60. 

Pan CC, Chen PC, Tsay SH, et al. Cytoplasmic immunoreactivity for thyroid transcription factor-1 in hepatocellular carcinoma a comparative immunohistochemical analysis of four commercial antibodies using a tissue array technique. Am. J. Clin. Pathol. 2004 121 343-349. 

In this chapter we show that topi inhibitors with apparently similar cytotoxic profile could have a specific effect of cell cycle, distinguishing the activity of classical camptothecin, such as irinotecan and SN-38, active specifically on the S phase by edotecarin active as tight binder on topi and without cell cycle specificity. The use of cytometry of solid tumors from xenograft made it possible to provide support to immunohistochemical analysis during the study of biomarkers, comparing cell cycle effects to topi modulation after treatment. 

The limited availability of suitable human tissue for experimental studies and the rather low CYP expression level in the human lung, compared to that found in rodents [111], have notably limited identification of the lung CYP isoforms. By means of RT-PCR, it has been possible to qualitatively demonstrate the presence of several CYP mRNAs in the human bronchial mucosa, namely CYP1A1, 2A6, 2B6, 2C, 2E1 and 3A5, whereas the expression of CYP1A2, 2D6, 3A4 and 3A7 could be detected only in some samples (Table 10.1) [109, 111, 112], Immunohistochemical analysis has shown the significant expression of CYP enzymes in different pulmonary cells including... 

Perfusion of brain tissue is not as easy to perform as immediate freezing following removal of fresh tissue from the animal. The former technique has long been preferred for immunohistochemistry, but it has been argued that fresh-frozen sections are better for analysis of mRNA distribution and can be adapted for immunohistochemical analysis (Newton et al., 2002). 

V/XI collagen genes/" col5al, col5a3, and coll la2. Immunohistochemical analysis suggested that type XXVII collagen localizes at the ossification site of hypertrophic cartilage/ ... 

The ABC detection system has been shown to be more sensitive than most other detection system (5,6), primarily because of the large size of the preformed ABC complexes, which result in amplification of the signals. Alternative detection systems for immunohistochemical analysis include the peroxidase-antiperoxidase (PAP) (1) and the alkaline phosphatase-antialkaline phosphatase (APAAP) systems (7) (see Chapter 24). These approaches are conceptually and technically similar, and will not be discussed here. 

Wick, M. R., Lillemoe, T. J., Copland, G. T., Swanson, P. E., Manivel, J. C., and Kiang, D. T. (1989) Gross cystic disease flnid protein-15 as a marker for breast cancer immunohistochemical analysis of 690 hnman neoplasms and comparison with alpha-lactalbnmin. Hum. Pathol. 20, 281-287. 

Srinivasan, U., Jones, E., Carolan, J., and Feighery, C. (2006). Immunohistochemical analysis of coeliac mucosa following ingestion of oats. Clin. Exp. Immunol. 144,197-203. 

Koshiba T, Hosotani R. Miyamoto Y, et al. Immunohistochemical analysis of cyclooxygenase-2 expression in pancreatic tumors. Int J Pancreatol 1999 26 69-76. 

Sibley, R. K., Sutherland, D. E., Goetz, F., and Michael, A. F. (1985). Recurrent diabetes mellitus in the pancrease iso- and allograft. A light and electron microscopic and immunohistochemical analysis of four cases. Lab. Invest. 53, 132-144. 

Londono, D.P., Alvarez, J.I., Trujillo, J., Jaramillo, M.M. and Restrepo, B.l. (2002) The inflammatory cell infiltrates in porcine cysticercosis immunohistochemical analysis during various stages of infection. Veterinary Parasitology 109, 249-259. 

Wood et al. (1991) have used the Southern hybridization method for detecting DNA amplification and a possible structural rearrangement of the HER-2/nen oncogene in 1 of 12 bladder tumors. Amplification of this oncogene in the tumor was sixfold that of oncogene found in placental DNA. Approximately 36% of the tumors studied overexpressed HER-2 mRNA, which was 3- to 38-fold that of normal urothelium. HER-2 overexpression occurred in superficial and invasive tumors. Deoxyribonucleic acid amplification occurs infrequently in bladder carcinoma, in contrast to its occurrence in some other carcinomas. Immunohistochemical analysis has shown that pi85 HER-2 polyclonal antibody is specific for HER-2 protein overexpression in bladder carcinoma. This study was carried out prior to the use of Herceptin. 

Esteban, J. M., Ahn, C., Battifora, H., and Felder, B. 1994c. Predictive value of estrogen receptors evaluated by quantitative immunohistochemical analysis in breast cancer. Am. J. Clin. Pathol. 702 S9-S12. 

Kashima, K., Yokoyama, S., Daa, T., Nakayama, I., Nickerson, P. A., and Noguchi, S. 1997. Cytoplasmic biotin-like activity interferes with immunohistochemical analysis of thyroid lesions A comparison of antigen retrieval methods. Mod. Pathol. 70 515-519. 

Martin de las Mulas, J., Millan, Y., Blankenstein, M. A., van Mil, F., and Misdorp, W. 2000. Immunohistochemical analysis of estrogen receptors in feline mammary gland benign and malignant lesions Comparisons with biochemical assay. Domestic Animal. Endocrinol. 78 111-125. 

Miura, K., Ueda, Y., Mineta, H., Takebayashi, S., Harada, H., Arabi, K., and Misawa, K. 2001. Immunohistochemical analysis of small cell carcinoma of the head and neck A report of four patients and a review of sixteen patients in the literature with ectopic hormone production. Ann. Otol. Rhinol. Laryngol. 770 76-82. 

Radhi, J. M. 2000. Immunohistochemical analysis of pleomorphic lobular carcinoma Higher expression of p53 and chromogranin and lower expression of ER and PgR. Histopathology 36 156-160. 

L. G. 2000. Immunohistochemical analysis of estrogen receptors in breast carcinomas using monoclonal antibodies that recognize different domains of the receptor molecule. Appl. Immunohistochem. Mol. Morphol. 8 275-284. 

VojteSek, B., Bartek, J., Midgley, C. A., and Lane, D. P. 1992. An immunohistochemical analysis of human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53. J. Immunol. Methods 757 237-244. 

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