Immunohistochemical expression analysis

Kommoss F, Schmidt D, Coetdt W, et al. Immunohistochemical expression analysis of inhibin-alpha and -beta subunits in pat-tial and complete moles, trophoblastic tumots, and endomettial decidua. Int J Gynecol Pathol. 2001 20 380-385. 

Takashima M, Ueki T, Nagai E, et al. Carcinoma of the ampulla of Vater associated with or without adenoma a clinico-pathologic analysis of 198 cases with reference to p53 and Ki-67 immunohistochemical expressions. Mod Pathol. 2000 13 1300-1307. 

Vang R, Gown AM, Barry TS, et al. Gytokeratins 7 and 20 in pti-mary and secondary mucinous tumors of the ovary analysis of coordinate immunohistochemical expression profiles and staining distribution in 179 cases. Am J Surg Pathol. 2006 30 1130. 

Evidence implicating CX3CR1 and fractalkine in atherosclerosis is growing. Immunohistochemical analysis of human atherosclerotic lesions demonstrate that macrophages in the intima as well as smooth muscle cells, mononuclear cells, and foam cells in the deep intima and media express CX3CL1, whereas normal arteries do not (57,58). 

It is well accepted that MDMA produces 5-HT depletions in rat CNS, but much less attention has been devoted to the effects of MDMA on established markers of neurotoxicity such as cell death, silver-positive staining, and reactive gliosis. Support for the hypothesis of MDMA-induced axotomy relies heavily on immunohistochemical analysis of 5-HT levels, which could produce misleading results if not validated by other methods. For example, MDMA-induced loss of 5-HT could be due to persistent adaptive changes in gene expression or protein function, reflecting a state of metabolic quiescence rather than neurotoxic damage. Table 7.3 summarizes the effects of MDMA on hallmark measures of neurotoxicity. 

OATP-C, a liver-specific member of this transporter family, was cloned by number of groups [17, 30-32] and often referred by aliases such as liver-specific transporter 1 (LST-1) and OATP2. Immunohistochemical analysis proved OATP-C expression on the basolateral membrane of hepatocytes [32]. As with OATP-A, OATP-C... 

Kelly D. and King T.P. (1991). The influence of lactation products on the temporal expression of histo-blood group antigens in the intestines of suckling pigs lectin histochemical and immunohistochemical analysis . Histochem J, 23, 55-60. 

Geertzema, A. Hennipman, and G. Rijksen, c-Src protein expression is increased in human breast cancer. A immunohistochemical and biochemical analysis, J. Pathol. 180 383 (1996). 

The limited availability of suitable human tissue for experimental studies and the rather low CYP expression level in the human lung, compared to that found in rodents [111], have notably limited identification of the lung CYP isoforms. By means of RT-PCR, it has been possible to qualitatively demonstrate the presence of several CYP mRNAs in the human bronchial mucosa, namely CYP1A1, 2A6, 2B6, 2C, 2E1 and 3A5, whereas the expression of CYP1A2, 2D6, 3A4 and 3A7 could be detected only in some samples (Table 10.1) [109, 111, 112], Immunohistochemical analysis has shown the significant expression of CYP enzymes in different pulmonary cells including... 

Koshiba T, Hosotani R. Miyamoto Y, et al. Immunohistochemical analysis of cyclooxygenase-2 expression in pancreatic tumors. Int J Pancreatol 1999 26 69-76. 

Hatanaka, Y., Hashizume, K., Kamihara, Y., Itoh, H., Tsuda, H., Osamura, R. Y., and Tany, Y. 2001. Quantitative immunohistochemical evaluation of HER2/neu expression with Herceptest in breast carcinoma by image analysis. Pathol. Int. 51 33-36. 

Kim, D., Gregory, C. W., Smith, G. J., and Mohler, J. L. 1999a. Immunohistochemical quantitation of androgen receptor expression using color video image analysis. Cytometry 35 2-10. 

Radhi, J. M. 2000. Immunohistochemical analysis of pleomorphic lobular carcinoma Higher expression of p53 and chromogranin and lower expression of ER and PgR. Histopathology 36 156-160. 

Both functional assays [vincristine transport (381) and rhodamine 123 transport (383)] and biochemical assays involving immunohistochemical analysis (381,384) have confirmed the expression of P-gp in the luminal membrane of BMECs cultured on polycarbonate membranes. Additionally, immunohistochemical methods showed the expression of P-gp in BMEC to be constant and at a high level in five- to seven-day-old old primary cultures (384). Like many other barrier-forming cells, BMECs appear to express other efflux proteins, for example, RT-PCR and immunoblot analysis have shown the presence of MRP1 in rat BMECs (385,386). Functional evidence has also been presented to confirm the expression of MRP1 in BMECs (387). 

Li Q-H, Nakadate K, Tanaka-Nakadate S, Nakatsuka D, Cui Y, Watanabe Y. Unique expression patterns of 5-HT2A and 5-HT2C receptors in the rat brain during postnatal development Western blot and immunohistochemical analysis. J Comp Neurol 2004 469 128-140. 

Not only biochemical analysis, but immunohistochemical staining with antibodies against gangliosides has similarly revealed the existence and distribution of gangliosides in epidermis. Nakakuma et al. showed that epidermal keratinocytes reacted with an anti-GM3 monoclonal antibody, but not an anti-GD3 mAb.11 Expression of GM3, predominantly in the stratum corneum, was reported by Paller et al.12 In contrast, Hersey et al. detected GD2 in the basal and spinous layers of the epidermis, whereas neither GM3 nor GD3 was detected in normal skin.13 However, the epidermis adjacent to naevi and primary melanoma strongly expressed GD3.13... 

MAbs make excellent tools with which to begin elucidating the relationship between chemokine, receptor, leukocyte recruitment, and disease. Flow cytometric analysis of chemokine receptor expression on peripheral blood leukocytes together with immunohistochemical examination of chemokine receptor expression on leukocytes recruited to inflammatory lesions may be used to correlate specific receptors with disease associated leukocyte infiltrates. 

Fig. 4.3 Immunohistochemical analysis in Brassica napus (A,C,E) and Arabidopsis thaliana (B,D) of myrosinase by use of the antibody 3D7. Myrosinase is present in the ground tissue of cotyledons (left) and radicle (upper right) of B. napus embryo (A), while in A. thaliana seed no expression is detected (B) some cells in the seed coat retain unoxidized substrate. Arrows in (A) show examples of myrosinase-containing myrosin cells. In A. thaliana leaves, myrosinase is located in phloem myrosin cells (D), whereas in B. napus leaves myrosinase is in myrosin cells in the phloem (C), as well as in the ground tissue (E). For experimental details, see1. The size bars equal 100 pm.

Autoradiography, in situ hybridization, western blot analysis, and immunohistochemical studies have described the distribution of sigma receptors in the brain (Samovilova et al., 1985 Largent et al., 1986 Jansen et al., 1991 Bouchard and Quirion, 1997 Alonso et al., 2000 Zamanillo et al., 2000). The al receptor is expressed in septum, olfactory bulb, hypothalamus, anterodorsal thalamic nucleus, dorsal raphe, locus coeruleus, substantia nigra, and the cerebellum. 

Direct immunohistochemical analysis of prostatic tissue has become very popular since the development of AR antibodies. However, a disadvantage of this technique in quantitative analysis is that the intensity of the immunohistochemical stain is dependent on the intactness of the structure of the AR. Therefore, mutations or alterations in the structure may reduce staining intensity (T5). Biochemical and immunohistochemical studies of AR content in relation to grade or stage of disease, as well as prediction of response to endocrine therapy, has been inconsistent. Nearly all primary prostate cancer specimens positively express AR protein, as determined by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) analysis as well as by immunohistochemical analysis on formalin-fixed, paraffin-embedded primary prostate tissues (D12, H14). In advanced-stage prostate cancer, immunohistochemical techniques has shown that metastases in bone, the... 

As shown in Table (13) , The reduction of the lymphocyte, CD4 and CD8 T cell numbers in LLC-bearing mice was also inhibited by the oral administration of A-l (30 and/or 300 mg/kg). From the immunohistochemical analysis in tumors, A-l (30, 100 and 300 mg/kg) increased the number of apoptotic cells in the tumors. In the untreated LLC-bearing mice, von Willebrand factor (vWF, a marker of angiogenesis) expression was increased in the tumors. Angiogenesis in the tumors was inhibited by the oral administration of A-l. 

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