Human cervical adenocarcinoma

In order to assess the effect of the corn cob xylan on the cell viability and proliferation rate, xylan solutions at concentrations of 0.1, 0.25, 0.50, 0.75, and 1 mg/ml were placed in contact with human cervical adenocarcinoma cells (HeLa cells) for 24 and 72 h. Finally, the cell viability was determined by the MTT assay. It was observed that regardless of the xylan concentration, the samples tested did not affect the viability of HeLa cells after incubation for 24 h (Figure 13) (Unpublished data). 

Houze (8) prepared pentafluorophenylsulfonanilide urea derivatives, (VIII), that were effective in arresting the growth of HeLa cells derived from a human cervical adenocarcinoma tumor cells. 

In spite of various and detailed studies of Aconitum alkaloids, very little information is available on their antitumor activity. De Ines et al. [46] have screened 43 Ci9-diterpenoid alkaloids on CT26 (murine colon adenocarcinoma), SW480 (human colon adenocarcinoma), HeLa (human cervical adenocarcinoma), SK-MEL-25 and SK-MEL-28 (human melanomas), and on the non-tumor cell line CHO (Chinese hamster ovary cells). Several compotmds (neoline, 8-0-methylco-lumbianine, 18-0-demethylpubescenine, lycoctonine, acetylajadine, etc.) exhibit... 

Gardasil is a noninfectious recombinant vaccine consisting of capsid proteins from four different human papillomaviruses (HPVs) of types 6,11, 16, and 18. HPV causes squamous cell cervical cancer and cervical adenocarcinoma, as well as 35-50% of vulvar and vaginal cancers. 

The antiproliferative and antitubulin activities of libraries of pyranopyridones and pyranoquinolones 147 (Scheme 28) derived from aldehydes, malononitrile and pyridones 146 have been examined [65]. The compounds were evaluated for their ability to reduce cell viability by 50% after 48 h of treatment relative to control. The HeLa and MCE-7 cell fines were used as models for human cervical and breast adenocarcinoma, respectively. Numerous compounds exhibited submicromolar antiproliferative activities with the quinolones being more potent than their pyridine counterparts (Eig. 20). Because of the structural similarity of pyranopyridones with chromene scaffolds known to inhibit tubulin polymerization, compounds 149 and 150 were further evaluated in Jurkat cells for their apoptotic... 

Human cervical epithelial adenocarcinoma (HeLa) cells (ATCC). 

Ishikawa M, Fujii T, Masumoto N, et al. Correlation of pl6INK4A overexpression with human papillomavirus infection in cervical adenocarcinomas. Int Gynecol Pathol. 2003 22 378-385. 

Raubenheimer reported the anti-cancer activity of compound 34 (Figure 4.9).This complex was tested on human cervical epithelioid carcinoma cell line HeLa, human colon adenocarcinoma cell line CoLo 320 DM, leukaemia cell line Jurkat, and breast cancer cell line MCF-7. IC50 values determined by the MTT assay showed human lymphocytes were less sensitive than the cancer cells tested, indicating some selectivity for the cancer cells. 

Several infeetions play a role in the pathogenesis of eaneer. Apart from Helicobacter pylori infection, which is a major environmental factor in the development of gastric adenocarcinoma, hepatitis B infeetion is reported to be assoeiated with hepatocellular carcinoma. Cervical squamous cell eaneer is associated with human papilloma vims. ... 

Fig. 2. Restriction enzyme analysis and Southern-blot hybridization of human tumor DNA. Human tumor DNA (20 p-g) was isolated (16) and digested with coRI restriction enzyme, electrophoresed on a 0.8% agarose gel (A shows the ethidium-bromide stain) and after Southern transfer, probed with P-labeled pcD-p(ADP-ribose) polymerase cDNA, (B). Lanes 1, Human placental DNA 2, Laryngeal squamous ceU carcinoma (SQ-20B) 3, Lung adenocarcinoma (A549) 4, Cervical carcinoma (HeLaSs) Ewing s sarcoma (A4573) 6, normal human fibroblasts (NHF). Marker-lambda-DNA.

Many researchers have conducted in vitro studies on the potential anticancer activity of flavones and flavonols in diverse cell systems. An array of 55 flavones having a variety of substituents was evaluated by Cushman and Nagarathnam for cytotoxicity in five cancer cell cultures, A-549 lung carcinoma, MCF-7 breast carcinoma, HT-29 colon adenocarcinoma, SKMEL-5 melanoma, and MLM melanoma [151]. Other reports also revealed that flavones and flavonols exhibited cytotoxicity in vitro to many human cell lines, including esophageal cancer cells [152, 153], colon cancer cells [154, 155], hepatoma cells [156, 157], prostate carcinoma cells [158, 159], and cervical carcinoma cells [160, 161],... 

The possible modes of action of 4HPR have been mostly investigated in already transformed tumor cells. 4HPR suppressed the proliferation of cell lines of different tumor types such as human breast carcinoma [24-27], prostate adenocarcinoma [28, 29], leukemias [30, 31], neuroblastoma [32, 33, 34], ovarian carcinoma [35, 36], cervical carcinoma [37, 38], head and neck squamous cell carcinoma [39], esophageal squamous carcinoma [40] and small-cell lung can-... 

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