Murine colon adenocarcinoma

CCR2 expression was also examined in liver tumor formation induced by intraportal injection of a murine colon adenocarcinoma cell line (58). Tumor growth occurred at similar rates in wild-type and CCR2 /" mice, but the number... 

Tan, M. H., Holyoke, E. D. and Goldrosen, M. H. (1977) Murine colon adenocarcinoma syngeneic orthotopic transplantation and subsequent hepatic metastases. J Natl Cancer Inst 59, 1537-1544. 

Sugimoto, Y., Watanabe, M., Oh-hara, T., Sato, S., Isoe, T. and Tsuruo, T. (1991). Suppression of experimental lung colonization of a metastatic variant of murine colon adenocarcinoma 26 by MoAb 8F11 inhibiting TCIPA. Cancer Res. 51, 921-925. 

Watanabe, M., Okochi, E., Sugimoto, Y. and Tsuruo, T. (1988). Identification of a platelet aggregating factor of murine colon adenocarcinoma 26, Mw 44,000 membrane protein as determined by MoAbs. Cancer Res. 48, 6411-6416. 

In accordance with this highly selective delivery to solid tumor sites, a dramatic enhancement of antitumor activity was observed (46). Figure 15 shows in vivo antitumor activity against murine colon adenocarcinoma 26. For free ADR, only the maximum tolerated dose (10 mg/kg body weight) provided considerable inhibition effects on tumor growth however, a decrease in tumor volume was never seen from the day of the first injection. For the polymeric micelles, the tumor completely disappeared in two doses (20 and 10 mg physically entrapped ADR/kg of body weight). All the polymeric... 

In spite of various and detailed studies of Aconitum alkaloids, very little information is available on their antitumor activity. De Ines et al. [46] have screened 43 Ci9-diterpenoid alkaloids on CT26 (murine colon adenocarcinoma), SW480 (human colon adenocarcinoma), HeLa (human cervical adenocarcinoma), SK-MEL-25 and SK-MEL-28 (human melanomas), and on the non-tumor cell line CHO (Chinese hamster ovary cells). Several compotmds (neoline, 8-0-methylco-lumbianine, 18-0-demethylpubescenine, lycoctonine, acetylajadine, etc.) exhibit... 

Fig. 2. Concentration-dependent inhibition of the growth of murine L1210 leukemia and rat RCC-2 colon adenocarcinoma cell populations by VBL ( ), deoxy VBL (O), epideoxy VBL ( ), and deoxydesethyl VBL (A).

B16 melanoma, Walker sarcoma, and M5076 forming liver metastasis have been used in the preclinical evaluation of HPMA copolymer-adriamydn conjugates [36]. Other tumors useful for secondary screening are MS-2 sarcoma, NMU-1 murine lung adenocarcinoma, and murine adenocarcinoma Colon 26. These have been used by Zunino et al. [147] to determine the activity of poly (carboxylic acid) immobilized anthracyclines. Mice inoculated intramuscularly with Lewis lung carcinoma have been used by Pratesi et al. [215] to assess the effect of a poly-L-aspartic acid/doxorubicin conjugate. 

Holgate ST (1999) The epidemic of allergy and asthma. Nature 402(Suppl 6760) B2-B4 Hoskin DW, Reynolds T, Blay J (1994a) Colon adenocarcinoma cells inhibit anti-CD3-activated killer cell induction. Cancer Immunol Immunother CII 38(3) 201-207 Hoskin DW, Reynolds T, Blay J (1994b) Adenosine as a possible inhibitor of killer T-cell activation in the microenvironment of solid tumours. Int J Cancer 59(6) 854-855 Hoskin DW, Reynolds T, Blay J (1994c) 2-Chloroadenosine inhibits the MHC-unrestricted cytolytic activity of anti-CD3-activated killer cells evidence for the involvement of a non-Aj/ A2 cell-surface adenosine receptor. Cell Immunol 159(1) 85—93 Hoskin DW, Buttler JJ, Drapeau D, Haeryfar SM, Blay J (2002) Adenosine acts through an A3 receptor to prevent the induction of murine anti-CD3-activated killer T cells. Int J Cancer 99(3) 386—395... 

The value of A app is tumor-dependent. It varies from 3.1 xlO 8 cm2/cmH20/sec in Morris hepatoma 5123 (Swabb et al., 1974) to 1.8xl0-6cm2/cmH2O/sec in a murine mammary carcinoma (MCalV) (Netti et al., 2000). The variation is likely due to the difference in tumor lines rather than experimental methods, because the same methods have also been used to quantify A app in a human colon adenocarcinoma (LS174T) in three different studies and the data from these studies are nearly identical (Boucher et al., 1998 Netti et al., 2000 Znati, 1995). 

Since natural product cryptolepine hydrochloride 173 (Fig. 48) has been found to be cytotoxic to B16 melanoma cells with an IC50 of 0.3 pg/mL (1.3 pM) [102], there has also been interest in cationic 5-carboline-based compounds as antitumor agents. For example, Wright and coworkers synthesized a series of these compounds and evaluated their activity against MAC 15 cells (murine adenocarcinomas of the colon) [103]. Of the compounds prepared, the most active was 179 which had an IC50 value of 1.03 pM. 

---