Carcinoma cell line

Embryonal carcinoma cell lines have also been established in culture from transplantable teratocarcinomas. The embryonal carcinoma (EC) stem cells present in the tumors actually differentiated, and give rise to a variety of different types of embiyonic and adult cells. When these EC cells are cultured, they still possess the... 

OITATE M, NAKAKI R, KOYABU N, TAKANAGA H, MATSUO H, OHTANI H, SAWADA Y (2001) TranSCellular transport of genistein, a soybean-derived isoflavone, across human colon carcinoma cell line (Caco-2). Biopharm Drug Dispos. 22 23-9. 

The amino acid sequence of the human erythrocyte glucose transporter was deduced from the nucleotide sequence of a cDNA clone in 1985 [106]. Polyclonal antibodies raised against the protein were used to screen a Xgtl I cDNA library prepared from the human hepatocellular carcinoma cell line HepG2. (Like many other transformed... 

CP-channels with smaller conductance have first been noted in the rectal gland of Squalus acanthias by ourselves and in the colonic carcinoma cell line HT29 [61,73]. Later these types of 5-15 pS CP-channels were also found in pancreatic ducts, A6-cells and many other cells [74,75]. It is now claimed that this kind of channel is much more relevant than the ICOR for the pathophysiology of cystic fibrosis [12]. 

Pinto, M. et al. (1983). Enterocyte-like differentiation an polarizationof the human colon carcinoma cell line Caco-2 in culture. Biol. Cell 47 323-330. 

Hoffmann, R, Ji, H., Moritz, R. L., Connolly, L. M., Frecklington, D. F., Layton, M. J., Eddes, J. S., Simpson, R. J. (2001). Continuous free-flow electrophoresis separation of cytosolic proteins from the human colon carcinoma cell line LIM 1215 a non two-dimensional gel electrophoresis-based proteome analysis strategy. Proteomics 1(7), 807. 

Hiribarren, A., Heyman, M., L Helgouac h, A. and Desjeux, J.F. (1993) Effect of cytokines on the epithelial function of the human colon carcinoma cell line HT29 cll9A. Gut 34, 616-620. 

Borchardt, R. T., Characterization of the human colon carcinoma cell line (Caco-2) as a model for intestinal epithelial permeability, Gastroenterology 1989, 96, 736-749. 

M. D., Kedinger, M., Triadou, N., Dussaulx, E., Lacroix, B., Simon-Assman, P., Happen, K., Fogh, J., Zweibaum, A., Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture, Biol. Cell 1983, 47, 323-330. 

Brandsch, M., Miyamoto, Y., Ganapathy, V., Leibach, F. H., Expression and protein C-dependent regulation of peptide/H+ co-transport system in the Caco-2 human colon carcinoma cell line, Biochem. J. 1994, 299, 253-260. 

Grasset, E., Pinto, M., Dussaulx, E., Z WEI BAUM, A., Desjeux, J.-F., Epithelial properties of human colonic carcinoma cell line Caco-2 electrical parameters, Am. J. Physiol. 1984, 247, C260-C267. 

Kuang TO et al. Differential screening and suppression subtractive hybridisation identified genes differentially expressed in an estrogen receptor-positive breast carcinoma cell line. Nucleic Acids Res 1998 26 1116-1123. 

Seow TK et al. Two-dimensional electrophoresis map of the human hepatocellular carcinoma cell line, HCC-M, and identification of the separated proteins by... 

Simpson RJ et al. Proteomic analysis of the human colon carcinoma cell line (LIM 1215] development of a membrane protein database. Electrophoresis 2000 21 1707-1732. 

Cells in vivo exist either attached to a surface or free in suspension. Adherent cell lines originate from cells of solid tissue. Breast carcinoma cell lines (such as MCF7, T47D, and SK-BR-3) are adherent cultures, and these cells are grown on the surface of plastic flasks that have been treated to facilitate adhesion (see Fig. 6.2). Suspension culture cell lines originate from cells that exist in suspension, such as those cells present in the blood and the lymphatic system (see Fig. 6.3). 

SK-BR-3 This is an adherent breast carcinoma cell line. This cell line is cultured in McCoy s 5A medium with 2mM glutamine and 10% Fetal Bovine Serum (FBS) at 37°C in a 5% C02 in air atmosphere. 

MDA-MB-453 This represents an adherent breast carcinoma cell line which is cultured in Leibovitz s L-15 medium with 2mM glutamine and 15% FBS at 37°C in atmospheric air. 

Formalin-fixed and paraffin-processed sections from commercially prepared composite cell line block comprising the human breast carcinoma cell lines SK-BR-3, MDA-MB-453, MDA-MB-175, and MDA-MB-231, have been used consistently for the past 5 years for HER2 IffC EQA evaluation. More recently, the same composite cell line block reference material has been introduced for EQA evaluation of HER2 ISH testing of breast cancer. The principles and protocols developed and established for running of these two schemes have been recently described by Miller et al.13 and Bartlett et al.14... 

Chang T, Hughes-Fulford (2009) Monolayer and spheroid culture of human liver hepatocellular carcinoma cell line cells demonstrate distinct global gene expression patterns and functional phenotypes. Tissue Eng Part A 15 559-567... 

Wang W, VanAlstyne PC, Irons KA, Chen S, Stewart JW and Birt DF. 2004. Individual and interactive effects of apigenin analogs on G2/M cell-cycle arrest in human colon carcinoma cell lines. Nutr Cancer 48(1) 106—114. 

Several companies have expressed the cDNA coding for human factor VIII C in a variety of eukaryotic production systems (human VIII C contains 25 potential glycosylation sites). CHO cells and BHK cell lines have been most commonly used, in addition to other cell lines, such as various mouse carcinoma cell lines. The recombinant factor VIII product generally contains only VIII C (i.e. is devoid of vWF). However, both clinical and preclinical studies have shown that administration of this product to patients suffering from haemophilia A is equally as effective as administering blood-derived factor VIII complex. The recombinant VIII C product appears to bind plasma... 

---