Fed-batch production

Fed-batch production of pyruvic acid [CH3COCOOH] from an engineered strain (Escherichia coli YYC202) was optimized by resorting to ED to prevent potential product inhibition in the bioreactor (Zelic et al., 2004). In this way, by continuous separation of pyruvate from the fermentation medium, high values of the pyruvate-to-glucose molar yield (1.78 mol/mol), volumetric productivity (145 kg m 3day ), and pyruvate concentration (79 kg/m3) were achieved by the repeated fed-batch mode. 

Fig. 8. Fed-batch production of citric acid from glucose by A. niger in a bubble column [43]. Bubble column 500-ml working volume temperature 35°C aeration 1.5 wm...

Dilsen S, Paul W, Sandgathe A, Tippe D, Freudl R, Thommes J, Kula M R, Takers R, Wandrey C, Weuster-Botz D (2000). Fed-batch production of recombinant human calcitonin precursor fusion protein using Staphylococcus carnosus as an expression-secretion system. Appl. Microbiol. Biotechnol. 54 361-369. 

Fig. 6.5.2 Biomass and aldolase concentration profiles for the fed-batch production of FucA...

Pandian SRK, Deepak V, Kalishwaralal K, Rameshkumar N, Jeyaraj M, Gurunathan S (2010) Optimization and fed-batch production of PHB utilizing dairy waste and sea water as nutrient sources by Bacillus megaterium SRKP-3. Bioresour Technol 101 705-711 Pantazaki AA, Papaneophytou CP, Pritsa AG, Liakopoulou-Kyriakides M, Kyriakidis DA (2009) Production of polyhydroxyalkanoates from whey by Thermus thermophilus HB8. Process Biochem 44 847-853... 

Weiner, M, Albermann, C., Gottlieb, K., Sprenger, GA., and Weuster-Botz, D. (2014) Fed-batch production of l-phenylalanine from glycerol and ammonia with recombinant Escherichia cdi. Biochem. Eng. /., 83, 62-69. 

Sun, Z., Ramsay, J.A., Guay, M., and Ramsay, B.A. (2007) Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates from nonanoic acid by Pseudomonas putida KT2440. Appl. Microbiol. Biotechnol, 74, 69-77. 

Dean, J. and Reddy, P. (2013) Metabolic analysis of antibody producing CHO cells in fed-batch production. Biotechnol Bioeng, 110, 1735-1747. 

There are two types of fed-batch production one results in a growth-associated product and the second results in a non-growth associated product. Cupriavidus necator has been reported to produce a nongrowth-associated product. In this type of production, there is a microbial growth phase followed by a PHA production phase... 

Zakaria and co-workers [28] reported the use of Comamonas sp. FBI 72 for the fed-batch production of copolymer poly(3HB-co-3HV) using a mixed organic acids treated palm oil mill effluent (POME) in a 2 1 bioreactor. A PHA content of 59 wt% and 3HV monomer composition of 21 mol% was obtained when the two-stage cultivation method was used. This method involves growing cells in a nutrient-rich medium for about 12 h, followed by inoculation of the production medium and supply of concentrated organic acids from treated POME using the fed-batch strategy. 

Sun Z, Ramsay J, Guay M, Ramsay B (2009) Fed-batch production of unsaturated medium-chain-length polyhydroxyalkanoates with controlled composition by Pseudomonas putida KT2440. Appl Microbiol Biotechnol (in press)... 

Chen X, Zhang DJ, Qi WT, Gao SJ, Xiu ZL, Xu P. (2003h). Microhial fed-batch production of 1,3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions. Appl Micmbiol Biotechnol, 63,143-146. 

Jun SA, Moon C, Kang CH, Kong SW, Sang BI, Um Y. (2010). Microbial fed-batch production of 1,3-propanediol using raw glycerol with suspended and immobilized Klebsiella pneumoniae. Appl Biochem Biotechnol, 161, 491-501. 

Corynebacterium glutamicum BOL-3/ pAN6- a/) Aerobic growth in minimal medium, then anaerobic fed-batch production in saline solution 1.67(1.36 ) 1134 21 (16") 107 (417) 0.10 Litsanov et al. (2012a)... 

Aerobic growth in complex 1.60 (1.60 ) medium, then anaerobic fed-batch production in complex medium... 

Bang SG, Choi CY. (1995). DO-stat fed-batch production of cis, cw-muconic acid from benzoic-acid by Pseudomonas putida Bm014. J Ferment Bioeng, 79, 381-383. 

Atif, et al. (2005). Fed batch production of hydrogen from palm oil mill effluent using anaerobic microflora. International Journal of Hydrogen Energy, 30, 1393—1397. 

CroUa A, Kennedy K. Fed-batch production of citric acid by Candida lipolytica grown on -paraffins. J Biotechnol 2004 110 73-84. 

The model described in Park and Ramirez (1988) for fed-batch production of a recombinant protein will serve as an example to outline the proposed methods. The mass balance equations take the following state space format... 

Lee and Ramirez (1994) have developed the optimal nutrient and inducer feeding strategy for the fed-batch production of induced foreign protein using recombinant bacteria. They used the optimal control theory and showed the existence of singular... 

He GQ, Kong Q, Chen QH, Ruan H (2005) Batch and fed-batch production of butyric acid by Clostridium butyricum ZJUCB. J Zhejiang Univ (Sci) 6(11) 1076-1080. doi 10.1631/Jzus. 2005.B1076... 

He, G.Q,Kong, Q., Chen, Q.-H., Ruan, H., 2005. Batch and fed-batch production of butyric add by QoshidiurafcMfi/ncum ZJUCB. Journal of Zhejiang University Science B 6 (11), 1076. 

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