Bacteria recombinant

Eaton RW, PJ Chapman (1992) Bacterial metabolism of naphthalene construction and use of recombinant bacteria to study ring cleavage of 1,2-dihydroxy naphthalene and subsequent reactions. J Bacteriol 174 7542-7554. 

Keywords. Polyhydroxyalkanoates, PHA biosynthesis genes, Recombinant bacteria, Fermentation... 

Table 1. Recombinant bacteria harboring the heterologous PHA biosynthesis genes and synthesized PHA... 

Figure 6.15 Left stress-induced response of native and recombinant bacteria. Right single E. coli cell fluorescent response after induction by mitomycin C. (Reproduced from ref. 16, with permission.)...

Paton, A. W., Morana, R., and Paton, J. C. (2001). Neutralization of shiga toxins Stxl, Stx2, and Stx2e by recombinant bacteria expressing mimics of globotriose and globotetraose. Infect. Immun. 69,1967-1970. 

J. Lee and W.F. Ramirez. Mathematical modeling of induced foreing protein production by recombinant bacteria. Biotechnol. Bioeng., 39 635-, 1992. 

Avidin is a glycoprotein which was initially extracted from chicken egg white and which exhibits four deep binding pockets for biotin. Streptavidin is an analogous protein with a molecular weight of about 70 kD which is prepared from the recombinant bacteria strain Streptomyces avidinii and which... 

This researcher is growing recombinant bacteria that will produce a compound to be tested as a drug for HIV infection. (David Parker/Photo Researchers, inc.)... 

As early as 1975, participants in the Asilomar conference expressed their concerns over the safety and wisdom of releasing GEMs. At that time the primary concern was accidental release, but we are now pursuing intentional releases. The successful release of several recombinant bacteria has done little to alleviate concerns over perceived ecological dangers (Halvorson et al., 1985). 

The enzyme can be produced cheaply and in large quantities by recombinant bacteria. 

Active compounds were retested in medium containing glycerol as a carbon source. Glycerol does not require PFK for introduction into bacterial energy metabolism pathways. Compounds that were as toxic to the recombinant bacteria in the presence of glycerol as they were in mannitol could not be acting via inhibition of PFK. Conversely, compounds that are significantly more toxic in... 

Fig.l. Biologic and chemical processes for production of R3HB in natural and recombinant bacteria. PhaA,P-ketothiolase PhaB,acetoacetyl-CoA reductase PhaC, PHA synthase Ptb, phosphotransbutyrylase Buk, butyratekinase TCA, tricarboxylic acid. 

Chemical processes for the production of RH As and RHA alkyl esters are composed of two steps (9,22) (Fig. 1). First, PHA is synthesized in natural or recombinant bacteria and then purified. Cells containing PHA can also be used. Second, purified PHA (or cells containing PHA) is depolymer-ized by acidic alcoholysis using sulfuric acid or hydrochloric acid as an acidic catalyst to produce RHA alkylesters. Subsequent saponification of the purified RHA alkylesters yields corresponding RHAs. 

Leclerc C, Charbit A, Martineau P, Deriaud E, Hofnung M, The cellular location of a foreign B cell epitope expressed by recombinant bacteria determines its T cell-independent or T cell-dependent characteristics, J. Immunol., 147 3545-3552, 1991. 

---