Proteins, foreign

Applications. Immunoassays are used in many different disciplines, having clinical, industrial, agricultural, and environmental appHcations. This technique has made possible rapid analysis of such varied analytes as vimses, toxins, hormones, foreign proteins, dmgs, and insecticides. 

Water soluble protein with a relative molecular mass of ca. 32600, which particularly contains copper and zinc bound like chelate (ca. 4 gram atoms) and has superoxide-dismutase-activity. It is isolated from bovine liver or from hemolyzed, plasma free erythrocytes obtained from bovine blood. Purification by manyfold fractionated precipitation and solvolyse methods and definitive separation of the residual foreign proteins by denaturizing heating of the orgotein concentrate in buffer solution to ca. 65-70 C and gel filtration and/or dialysis. 

Van den Broek, G., Timko, M.P., Kausch, A.P., Cashmore, A.R., Van Montagu, M. Herrera-Estrella, L. (1985). Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-biphosphate carboxylase. Nature, 313, 358-63. 

Ikeda, K., Nakazawa, H., Shimo-Oka, A. et al. (2006) Immobilization of diverse foreign proteins in viral polyhedra and potential application for protein microarrays. Proteomics, 6 (1), 54—66. 

Foreign Protein Expression Using Plant Cell Suspension and Hairy Root Cultures... 

Plant-based production systems are now being used commercially for the synthesis of foreign proteins [1-3]. Post-translational modification in plant cells is similar to that carried out by animal cells plant cells are also able to fold multimeric proteins correctly. The sites of glycosylation on plant-produced mammalian proteins are the same as on the native protein however, processing of N-linked glycans in the secretory pathway of plant cells results in a more diverse array of glycoforms than is produced in animal expression systems [4]. Glycoprotein activity is retained in plant-derived mammalian proteins. 

Production of Foreign Proteins Using Plant Tissue Culture... 

In this review, we focus on the use of plant tissue culture to produce foreign proteins that have direct commercial or medical applications. The development of large-scale plant tissue culture systems for the production of biopharmaceutical proteins requires efficient, high-level expression of stable, biologically active products. To minimize the cost of protein recovery and purification, it is preferable that the expression system releases the product in a form that can be harvested from the culture medium. In addition, the relevant bioprocessing issues associated with bioreactor culture of plant cells and tissues must be addressed. 

Extensive research has been carried out into the molecular aspects of foreign protein production in whole plants to enhance the yield, quality and stability of the product and to facilitate protein separation and purification from the biomass [3, 6, 9], In contrast, comparatively little research has been undertaken to investigate the... 

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