Klebsiella pneumoniae

Resistant Gram-negative organisms such as Klebsiella pneumoniae and Enterobacter aerogenes. 

AN AMPEROMETRIC ENZYME IMMUNOSENSOR BASED ON SCREEN-PRINTED ELECTRODE FOR THE DETERMINATION OF KLEBSIELLA PNEUMONIAE BACTERIAL ANTIGEN... 

The aim of our investigation was the development of the amperometric enzyme immunosensor for the determination of Klebsiella pneumoniae bacterial antigen (Ag), causes the different inflammatory diseases. The biosensing pail of the sensors consisted of the enzyme (cholinesterase) and antibodies (Ab) immobilized on the working surface of the screen-printed electrode. Bovine seiaim albumin was used as a matrix component. 

It was established that Ab to Klebsiella pneumoniae didn t demonstrate the cross-reactivity to antigens of the relative bacterial species so, it could be considered that antibodies investigated was highly specific only to the own antigen. The physical-chemical characteristics of the immunological interaction such as constants of formation of Ag-Ab complex were obtained. The binding constants of immune complex were Ka =(9.7 l.l)-10 and Ka,=(1.7+0.3)T0 (mg/ml)f... 

The developed amperometric enzyme immunosensor was probed to determine the Klebsiella pneumoniae antigen in the human sera samples. The obtained results were juxtaposed with the data of the bacteriological analysis. 

I Citrobacter freundii Klebsiella pneumoniae Enterobacter cloacae Yersinia enterocolitica... 

Kanamydn A Kanamycin B Escherichia coli Klebsiella pneumoniae Pseudomonas aeruginosa... 

Meropenem (Merrem IV) inhibits syndiesis of die bacterial cell wall and causes die deadi of susceptible cells. This drug is used for intra-abdominal infections caused by Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and odier susceptible organisms Meropenem also is effective against bacterial meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Hemophilus influenzae. 

The nitrogenase proteins are generally characterized by two letters indicating the species and strains of bacteria and the numerals 1 for the MoFe protein and 2 for the Fe protein. Thus, the Fe protein from Azotobacter vinelandii is Av2 and the MoFe protein from Klebsiella pneumoniae is Kpl. 

Figure 3 shows the three-dimensional structure of the MoFe protein from Klebsiella pneumoniae, Kpl, obtained at 1.65-A resolution (7). The overall structure of the polypeptides is frilly consistent with that reported earlier for Avl (3). The a and /8 subunits exhibit similar polypeptide folds with three domains of parallel /3 sheet/a helical type. At the interface between the three domains in the a subunit is a wide shallow cleft with the FeMoco at the bottom of the cleft about 10 A from the solvent. FeMoco is enclosed within the a subunit. The P cluster, however, is buried within the protein at the interface between the a and /8 subunits, being bound by cysteine residues from each subunit. A pseudo-twofold rotation axis passes between the two halves of the P cluster and relates the a and (3 subunits. Each af3 pair of subunits contains one FeMoco and one P cluster and thus appears... 

Fig. 3. The tetrameric structure of the MoFe protein (Kpl) from Klebsiella pneumoniae (7). The two FeMoco clusters and the P clusters are depicted by space-filling models and the polypeptides by ribbons diagrams (MOLSCRIPT (196)). The FeMoco clusters are bound only to the a subunits, whereas the P clusters span the interface of the a and j8 subunits.

Fig. 7. The nitrogen fixation (nif) genes of Klebsiella pneumoniae Eire contiguous on ca. 23 kb of chromosomal DNA. The EU rowheads indicate transcription stEuts.

Functions of Nitrogen Fixation (jiif) Gene Products in Klebsiella pneumoniae... 

Attaches to apoNifK2D2 to assist FeMoco insertion in Klebsiella pneumoniae-, Em equivalent protein inAzotobacter vinelandii is y Cysteine desulfurase... 

As mentioned earlier, a-D-carba-galactopyranose (1) has been found in a fermentation broth of Streptomyces sp. MA-4145, as an antibiotic. The potency of the antibiotic was rather low. A concentration of—125 fig/mh is required in order to produce a standard inhibition zone of 25-mm diameter against Klebsiella pneumoniae MB-1264, using 13-mm assay discs in a disc-plate assay. A sample of the synthetic a-DL-carba-galactopyranose (17) was... 

Escherichia coli and Klebsiella pneumoniae subsp, aerogenes produce acid from lactose on this medium, altering the colour of the indicator, and also adsorb some of the indicator which may be precipitated around the growing cells. The organisms causing typhoid and paratyphoid fever and bacillary dysentery do not ferment lactose, and colonies of these organisms appear transparent. 

Klebsiella pneumoniae subsp, aerogenes is found in the gut and respiratory tract of man and animals, and in soil and water. It may be distinguished from E. coli by a pattern of biochemical tests (Table 1.3). It can give rise to acute bronchopneumonia in humans but is not a common pathogen. 

Morse L. J., Williams H.I., Grenn F.P., Eldridge E.F. Rotta J.R. (1967) Septicaemia due to Klebsiella pneumoniae originating from ahandcream dispenser. NEnglJMed, 277, 472-473. 

Baarschers WH, AI Bharaty, J Elvish (1982) The biodegradation of methoxychlor by Klebsiella pneumoniae. Can J Microbiol 28 176-179. 

McBride KE, JW Kenny, DM Stalker (1986) Metabolism of the herbicide bromoxynil by Klebsiella pneumoniae subspecies ozaenae. Appl Environ Microbiol 52 325-330. 

Dihydroxyphenylacetate extradiol dioxygenase from Klebsiella pneumoniae strain M5al contains magnesium and is not activated by Fe (Gibello et al. 1994). 

Gibello A, E Ferrer, M Martin, A Garroido-Pertierra (1994) 3,4-Dohydroxyphenylacetate 2,3-dioxygemase from Klebsiella pneumoniae, a Mg + containing dioxygenase involved in aromatic catabolism. Biochem J 301 145-150. 

Wray JW, RH Abeles (1995) The methionine salvage pathway in Klebsiella pneumoniae and rat liver. J Biol Chem 270 3147-3153. 

Karns JS, RW Eaton (1997) Genes encoding i-triazine degradation are plasmid-borne in Klebsiella pneumoniae strain 99. J Agric Food Chem 45 1017-1022. 

Nawaz MS, TM Heinze, CE Cerniglia (1992) Metabolism of benzonitrile and butyronitrile by Klebsiella pneumoniae. Appl Environ Microbiol 58 27-31. 

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