Dehydrogenases glucose phosphate dehydrogenase

Glucose phosphate isomerase 5.3.1.9 C Fructose-6- phosphate NADP+ Glucose-6- phosphate Glucose-6-phosphate dehydrogenase... 

The preparation of these solutions is described in the text in section 4.6.7.2. Pipette these successively into 1-cm cuvettes. The absorbance of the mixtures is read twice (at 340 nm) before (ODi) and after (OD2) the addition of glucose-phosphate isomerase. G6P-DH glucose-6-phosphate dehydrogenase, HK hexokinase, NADP nicotinamide adenine dinucleotide, PGI glucose phosphate isomerase, TEA triethanolamine... 

Glucose -phosphate Dehydrogenase Origin Leuconostoc mesenteroides... 

Combined glucose phosphate isomerase and glucose-6-phosphate dehydrogenase deficiency of eiythroc5d es. 

En mes that use NADPVNADPH Glucose phosphate dehydrogenase Catalyzes reactions in the pentose phosphate pathway... 

For the synthesis of vanillin itself, there follows, in a separate step, a further enzymatic reduction ofthe carboxylic function. To recover the NADP+, the reaction product is stirred for 7 hours at 30 °C together with glucose in the presence of the arylaldehyde-dehydrogenase from Neurospora crassa and glucose phosphate dehydrogenase. 

This is an enzyme of glycogen synthesis which can be estimated by u.v. spectroscopy using phosphoglucomutase in an auxiliary reaction and glucose -phosphate dehydrogenase in an indicator reaction [644]. 

In this chapter a number of variations employed for the metabolism of carbohydrates has been described. These illustrate the many mechanisms devised to support the metabolism of organisms at the expense of fermentation or oxidation of carbohydrate. Many more individual reactions are known. For example, several additional reactions for metabolizing various pentoses have been reported. The specificity of various enzyme systems is shown by oxidation of free arabinose by Pseudomonas, which follows the general mechanism described for glucose phosphate in this organism, but the dehydrogenase and lactonizing... 

Clinical Analysis. A wide range of clinically important substances can be detected and quantitated using chemiluminescence or bioluminescence methods. Coupled enzyme assay protocols permit the measurement of kinase, dehydrogenase, and oxidases or the substrates of these enzymes as exemplified by reactions of glucose, creatine phosphate, and bile acid in the following ... 

Chemiluminescence and bioluminescence are also used in immunoassays to detect conventional enzyme labels (eg, alkaline phosphatase, P-galactosidase, glucose oxidase, glucose 6-phosphate dehydrogenase, horseradish peroxidase, microperoxidase, xanthine oxidase). The enhanced chemiluminescence assay for horseradish peroxidase (luminol-peroxide-4-iodophenol detection reagent) and various chemiluminescence adamantyl 1,2-dioxetane aryl phosphate substrates, eg, (11) and (15) for alkaline phosphatase labels are in routine use in immunoassay analyzers and in Western blotting kits (261—266). 

Two or more linked enzyme reactions can lead to a change in the concentration of NADH or NADPH that is equivalent to the concentration of the original analyte. The reference glucose measurement using hexokinase [9001-51-8] and glucose-6-phosphate dehydrogenase [9001-40-5] is an example ... 

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