Glyceraldehyde-3-phosphate dehydrogenase isolation

Ercolani, L., Florence, B., Denaro, M., Alexander, M. (1988). Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene. J. Biol. Chem. 263, 15335-15341. 

Two new immunophilins have recently been discovered which contain both FKBP and cyclophilin domains. A 37 kDa protein isolated from the Jurkat T-cell line was found to bind FK506, rapamycin, and cyclosporin A, all with high affinity.50 FKBP37 contains two FKBP domains and one cyclophilin domain, and also possesses glyceraldehyde 3-phosphate dehydrogenase activity. The 52 kDa protein, isolated from human lymphoid cells, also binds all three immunosuppressant drugs but shows no detectable rotamase activity towards a variety of peptidic substrates.95... 

Pawluk, A. Scopes, R.K. Griffiths-Smith, K. Isolation and properties of the glycolytic enzymes from Zymomonas mobilis. The five enzymes from glyceraldehyde-3-phosphate dehydrogenase through to pyruvate kinase. Biochem. J., 238, 275-281 (1986)... 

Figure 1. The rate of incorporation of carbon from Glc6P and pyruvate (ImM) into fatty acids and the rate of pyruvate (ImM) uptake by plastids isolated from embryos at three stages in development (A 1.5 mgFW, B 2.5 mgFW and C 3.5 mgFW). A unit of GAPDH (NAPH-dependent glyceraldehyde 3-phosphate dehydrogenase) activity is one />tmol min Each value is the mean SE of measurements from three separate preparations.

The natural substrate for the dehydrogenase, glyceraldehyde-3-phosphate (G-3-P), had been synthesized earlier by Hermann Fischer, Emil Fischer s son, and Baer in 1932. In 1934 Meyerhof and Lohmann synthesized hexose diphosphate, establishing it to be fructose 1,6 bisphosphate (F-l, 6 bis P). With F-1,6 bisP as substrate and hydrazine to trap the aldehydic and ketonic products of the reaction, G-3-P was identified in the mixture of G-3-P and dihydroxyacetone phosphate which resulted. Triose phosphate isomerase was then isolated and the importance of phosphorylated 3C derivatives established. 

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