NADP-glyceraldehyde-3-phosphate dehydrogenase

Enzyme activities of nonreversible NADP-glyceraldehyde 3-phosphate dehydrogenase and NADP-isocitrate dehydrogenase as marker for the cytosol, hydroxypyruvate reductase for peroxisomes and cytochrom c reductase for mitochondria were 0.3 to 2% compared to those of the total leaf extract. 

Abbreviations DAHP, deoxy-D-arablnoheptulosonate-7-phoshate DHAP, dihydroxyacetone phosphate E ase, 2-phosphoglycerate hydrolyase, enolase rev. NADP GAPDH, reversible NADP glyceraldehyde 3-phosphate dehydrogenase ... 

Aside from PEPCase, a number of other CAM-related genes have been partially characterised (Table 1). These include cDNA clones for pyruvate, orthophosphate dikinase (PPDK), a specific NADP malate dehydrogenase (MDH), glyceraldehyde phosphate dehydrogenase (GaPDH) and NADP-dependent malic enzyme (MOD). Previous studies indicated that the enzymatic activities of these gene products increased upon salt stress in the ice plant (Holtum Winter, 1982). As in the case... 

Ribulosebisphosphate carboxylase [Carboxy-dismutase] (4.1.1.39) is present. Glyceraldehyde-phosphate dehydrogenase (NADP )... 

Glyceraldehyde-3-phosphate dehydrogenase (NADP+) (nonphosphorylating) [EC 1.2.1.9], also referred to as triose-phosphate dehydrogenase, catalyzes the reaction of D-glyceraldehyde 3-phosphate with NADP+ and water to produce 3-phospho-D-glycerate and NADPH. 

Fructose-1,6-bisphosphatase Sedoheptulose-1,7-bisphosphatase Glyceraldehyde-3-phosphate dehydrogenase NADP-malate dehydrogenase Phosphoribulokinase Thylakoid ATP-synthase... 

For glyceraldehyde-3-phosphate dehydrogenase, glutamate dehydrogenase, and the NADP-linked oxidative decarboxylases, which have three substrates in one direction, initial rate measurements with a fixed concentration of any one of the three substrates also conform to Eq. (1). This again rules out any form of enzyme-substitution mechanism in which free product is formed before all the substrates have combined with the enzyme and indicates the involvement of a quaternary enzyme complex. The appropriate generalized form of Eq. (1) is... 

---