Gluconic acid 6-phosphate dehydrogenase

Adding glucose-6-phosphate dehydrogenase (G-6-PDH) in the presence of oxidized nicotinamide adenine trinucleotide (NADH) yields gluconic acid-6-phosphate and an equivalent amount of the reduced form, NADPH. The amount of NADPH can be measured from its absorbance at 340 nm. For fructose the first reaction is ... 

A purified D-glucose dehydrogenase, isolated from animal liver, catalyzes the oxidation of D-glucose to D-gluconic acid.202 A coenzyme (either nicotinamide adenine dinucleotide or its 2 -phosphate) is necessary for the oxidation. The reaction is reversible, and is specific for /J-D-glucopyranose it does not proceed with the a-D anomer, and it occurs only slowly with D-xylose. 

Despite the fact that the formation of these alkoxo and aryloxo vanadates is not favored relative to many other ligated species, they can still have important influences in enzymic systems. It has, for instance, been shown that vanadate in the presence of glucose and glucose-6-phosphate dehydrogenase readily produces gluconic acid, a normal product of glucose-6-phosphate metabolism [3], Similar reactivity has been observed with a number of enzymes that metabolize phosphate compounds [4],... 

Biologically relevant vanadate esters (33-35) and a mixed vanadate-phosphate anhydride (36) Ad = adenosyl. For 33, the oxidation of activated glucose to gluconic acid (the lactone form is shown), as catalysed hy glucose-6-phosphate dehydrogenase (Glu-6PDH), is also depicted. 

Pyridine nucleotide-dependent dehydrogenases (C 2.1) transform aldose derivatives to uronic acids, e.g., UDP-D-glucose to UDP-D-glucuronic acid and in animals also to aldonic acids, e.g., D-glucose-6-phosphate to D-gluconic acid-6-phos-phate (glucose-6-phosphate dehydrogenase). 

Glucose 6- hosph(Ue Dehydrogenase. The establishment of the mechanism of glycolysis led to the acceptance of this sequence as the major pathway of carbohydrate metabolism. However, one of the first enzymes to be identified and studied catalyzes a reaction that takes hexose phosphate on a different route. Warburg and Christian found an oxidative enz3rme that used a cofactor, later identified as TPN, to form 6-phospho-gluconic acid from glucose-6-phosphate. At the time these observations... 

D-Gluconic acid 6-phosphate (8), (20) Purification of glycerol 3-phosphate dehydrogenase 83... 

Gluconic acid 6-phosphate may be obtained from glucose 6-phosphate with a dehydrogenase and TPN. Glucosamine 6-phosphate can be prepared from glucose 6-phosphate and glutamine. 

Phospho-D-gluconic acid is formed from gluconic add by yeast glucono-kinase and from glucose 6-phosphate by the corresponding dehydrogenase or by bromine oxidation ... 

G, and Naresh Kumar, G. (2013) 2-Ketogluconic acid secretion by incorporation of Pseudomonas putida KT 2440 gluconate dehydrogenase (gad) operon in Enterobacter asburiae PSI3 improves mineral phosphate solubilization. Curr. Microbiol, 67 (3), 388-394. 

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