Glutamate phosphate dehydrogenase

Nicotinamide Adenine Dinucleotide phosphate (NADP+) Glutamate dehydrogenase, Alcohol dehydrogenase, Aryl-aldehyde dehydrogenase... 

Very low concentrations of substrates may be assayed by recycling the test substrate for an appreciable but definite period of time and measuring the amount of product formed. The coenzyme NADPH, for instance, may be assayed using the two enzymes glutamate dehydrogenase (EC 1.4.1.3) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) ... 

J.-C. Brochon, P. Wahl, J. M. Jallon, and M. Iwatsubo, Pulse fluorimetry study of beef liver glutamate dehydrogenase-reduced nicotinamide adenine dinucleotide phosphate complexes,... 

Terms in bold are defined in aminotransferases 660 transaminases 660 transamination 660 pyridoxal phosphate (PLP) 660 oxidative deamination 661 l-glutamate dehydrogenase 661 glutamine synthetase 662 glutaminase 663 creatine kinase 664... 

Glucose 6-phosphate isomerase 279 Glucosidases—see lysozyme Glutamate dehydrogenase 167 Glutamine synthetase 298 Glyceraldehyde 3-phosphate... 

Carbamoyl phosphate synthetase, which is technically not a member of the urea cycle, catalyzes the condensation and activation of ammonia (from the oxidative deamination of glutamate by glutamate dehydrogenase Topic M2) and C02 (in the form of bicarbonate, HC03 ) to form carbamoyl phosphate. The hydrolysis of two ATP molecules makes this reaction essentially irreversible. 

Chen S-S, Engel PC (1975) The equilibrium position of the reaction of bovine fiver glutamate dehydrogenase with pyridoxal 5 -phosphate. Biochem J 147 351-358... 

Ahmad, I., and HeUebust, J. A. (1989). A spectrophotometric procedure for measuing oxoglutarate and determining aminotransferase activities using nicotinamide adenine dinucleotide phosphate-hnked glutamate dehydrogenase from algae. Anal. Biochem. 180, 99—104. 

Ornithine and citrulline are amino acids, but they are not used as building blocks of proteins. The formation of NH4 + by glutamate dehydrogenase, its incorporation into carbamoyl phosphate, and the subsequent synthesis of citrulline take place in the mitochondrial matrix. In contrast, the next three reactions of the urea cycle, which lead to the formation of urea, take place in the cytosol. 

---