Glycerol-3-phosphate dehydrogenase EC

This enzyme utilizes in-glycerol 3-phosphate (L-glycerol 3-phosphate) and the 4-pro-S position of the coenzyme [38]. The primary structure of the rabbit muscle enzyme 

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Glycerol-3-phosphate dehydrogenase [EC 1.1.99.5] is a flavoprotein that catalyzes the reaction of sn-glycerol 3-phosphate with an acceptor substrate to produce glycerone phosphate (dihydroxyacetone phosphate) and the reduced acceptor. 

Glycerol-3-phosphate may be used as a substrate for the MRC in a subset of tissues. It is conveniently added after malonate when measuring either complex II (allowing measurement of glycerol-3-phosphate dehydrogenase EC 1.1.95.5) or complex II—III... 

Fig. 17. Nucleotidyltransferase substrate screening assay (NUSSA). A Nucleotide sugar-synthesizing nucleotidyltransferase (EC 2.7.7), B PPrdependent phosphofructokinase (EC 2.7.2.90), C Aldolase (EC 4.1.2.13), D Triose-phosphate isomerase (EC 5.3.1.1), E Glycerol-3-phosphate dehydrogenase (EC 1.1.1.8) [336]...

All assay methods are based on the forward ALD-catalyzed reaction. Both photometric fixed-time and continuous-monitoring procedures have been developed. In the analytical approach on which all the commonly used procedures and kits are based, the ALD reaction is coupled with two other enzyme reactions. Triosephosphate isomerase (EC 5.3.1.1) is added to ensure rapid conversion of all GLAP to DAP. Glycerol-3-phosphate dehydrogenase (EC 1.1.1.8) is added to reduce the DAP to glycerol-3-phosphate, with NADH acting as hydrogen donor. The decrease in NADH concentration is then measured. 

L-Glycerol-3-phosphate dehydrogenase (GDH, from rabbit muscle) [9075,65-4] M4 78,000 [EC 1.1.1.8]. Recrystd by adding (NH4)2S(>4 till 0.45 saturation at pH 5.5 at 4° and the small amount of ppte is removed then satd (NH4)2S04 is added dropwise from time to time over several days in the cold room. 

L-Glycerol-3-phosphate dehydrogenase (GDH, from rabbit muscle) [9075-65-4] Mr 78,000 [EC 1.1.1.8]. Recrystd by adding (NH4)2S04 till 0.45 saturation at pH 5.5 at 4° and the small amount of ppte is removed then satd (NH4)2S04 is added dropwise from time to time over several days in the cold room. The crystals are collected and recrystd until they have maximum activity. The enzyme is stable in half saturated (NH4)2S04 for several weeks at 4°. The equilibrium [dihydroxyacetone][NADH][H ]/[G-3-P][NAD] is 1.0 x lO M in Tris buffer at 25°. It uses NAD ten times more efficiently than NADP. The Km for G-3-P is 1.1 x 10 M, for NAD it is 3.8 x lO M and for dihydroxyacetone it is 4.6 x lO M in phosphate buffer pH 7.0 and at 23.3°. Dihydroxyacetone phosphate and fructose-1,6-diphosphate are inhibitors. [Branowski J Biol Chem 180 515 1949, The Enzymes 7 85 1963, Young and Pace Arch Biochem Biophys 75 125 1958 Walsh and Sallach Biochemistry 4 1076 1965.]... 

L-Glycerol-3-phosphate dehydrogenase (GDH, from rabbit muscle) [9075-65-4] 78,000 [EC 1.1.1.8]. 

Fig. 2. Acylglycerols. Biosynthesis of triacylglycer-ols in liver and adipose tissue cells. EC 1.1.1.8 Glycerol-3-phosphate dehydrogenase (NAD "). EC 2.3.1.15 (j ycerol-3-phosphate acyltransferase. EC 2.3.1.20 Diacylglycerol acyltransferase. EC 2.3.1.51 1-AcylgIycerol-3-phosphate acyltransferase. EC 3.1.3.4 Phosphatidate phosphatase. EC 6.2.1.3 Long-chaln-fatty acid-CoA ligase.

Fig. 4. Histidine tnosynthess. Intermediates PRPP, phosphoribosyl pyrophosidiate PR-ATP, JV-phoqjhoribosyl-ATP PR-AMP, A/ -phosphoribosyl-AMP PR-FAIC-RN, phosphoribosyl for-mimino-S-amino imidazole carboxamide ribonucleotide PRu-FAIC-RN, phosphoribulosyl for-mimino-5-aniino imidazole carboxamide ribonucleotide IGP, imidazole glycerol phosphate lAP, imidazole acetol idrosphate HP, histidinol (dioqrhate HOL, histidinol. Enzymes (I) ATP phos-phoribosyltransferase (EC 2.4.2.17) (2) phosphoribosyl-ATP pyrophosphohydrolase (EC 3.6.1.31) (3) idiospharibosyl-AMP cyciohydrolase (EC 3.5. A19) (4) PR-FAIC-RN isomerase (EC S.3.1.16) (S) glutamine amidotransferase (6) cyclase (7) imidazole glycerol phosphate dehydratase (EC 4.2.1.19) (8) histidinol-phosphate aminotransferase (EC 2.6.1.9) (9) histidinol phosphatase (EC 3.1.3.15) (10) histidinol dehydrogenase (EC 1.1.1.23).

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