D-Glucose-6-phosphate dehydrogenase

G9. Glaser, L., and Brown, D. H., Purification and properties of D-glucose-6-phosphate dehydrogenase. ]. Biol. Chem. 216, 67-79 (1955). 

Figure 18-24 Some reactions by which superoxide anions, hydrogen peroxide and related compounds are generated by neutrophils and to a lesser extent by other cells (a) NADPH oxidase, (b) superoxide dismutase, (c) catalase, (d) glucose-6-phosphate dehydrogenase,...

D-Glucose-6-phosphate dehydrogenase, D-gluconate-6-phosphate dehydrogenase, KDO-8-phosphate synthase, KDO-8-phosphate phosphatase and CMP-KDO synthetase have been purified to homogeneity and characterized. Munson, Rasmussen and Osborn (15) have partially purified one KDO transferase. D-Arabinose-5-phosphate isomerase is very unstable and has only been purified about 100 fold. D-Ribose-5-phosphate isomerase activity is approximately 20x that... 

Note HxK, G6PDH, GlcDH, and GaK are hexokinase, D-glucose-6-phosphate dehydrogenase, glucose dehydrogenase, and gluconate kinase. 

D. Glucose 6-phosphate dehydrogenase deficient individuals are at risk for hemolytic anemia. 

Total phosphorus is determined as inorganic phosphate after treatment with hot perchloric acid. Phosphorus in D-glucose-6-phosphate residues is assayed using D-glucose-6-phosphate dehydrogenase. 

Conditions have been established for the measurement of D-glucose using the hexokinase-D-glucose 6-phosphate dehydrogenase method. This has enabled a new approach to be made in kinetic analyses in which the dependencies upon experimental variables are much closer to equilibrium methods than to the more common kinetic methods. 

The evaluation of a procedure for D-glucose determination using hexokinase and D-glucose 6-phosphate dehydrogenase co-immobilized inside a nylon tube has been reported. The practical and economic feasibility of the system for use in a small laboratory was assessed. 

Figure 3. Synthesis of vanillin from DHS. Enzymes (a) DHS dehydratase (aroZ) (b) catechol-O-methyltransferase (comt) (c) aryl-aldehyde dehydrogenase (d) glucose 6-phosphate dehydrogenase, glucose 6-phosphate.

D-Glucose 6-phosphate dehydrogenase reacted with Sephadex cyclic imido-carbonate, either in the presence or in the absence of amino-acids, to give an active immobilized form of the enzyme.Sephadex activated with either cyanogen bromide or trichloro-l,3,5-triazine reacted with trypsin to give an active immobilized form of the enzyme. ... 

Co-immobilization with D-glucose-6-phosphate dehydrogenase by reaction with agarose cyclic imidocarbonate... 

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