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Anthocyanins extraction

Use of Extracted Anthocyanin Derivatives in Nanostructures for Solar Energy Conversion... [Pg.245]

This is the classical method of extracting anthocyanins from plant materials. This procedure involves maceration or soaking of the plant material in methanol containing a small concentration of mineral acid (e.g., HC1). Methanol extraction is a rapid, easy, and efficient method for anthocyanin extraction. However, a crude aqueous extract with several contaminants is obtained, and methanol evaporation can result in hydrolysis of labile acyl linkages, which is aggravated by the presence of HC1. [Pg.777]

The extracted anthocyanin-containing solution can be used for pigment quantitation by the pH-differential method (unit fi.2). Both the weight of the fresh material and the final volume of the extract should be accurately recorded when further analyses of pigment content will be carried out. [Pg.783]

Extract anthocyanins from 700 g cherries using 900 ml of 99.9 0.1 (v/v) metha-nol/HCl three times. [Pg.825]

In a polyphenolic extract, anthocyanins can interfere with other polyphenolics such as pro-cyanidins during HPLC analysis and hence should be removed prior to analysis. Anthocyanins from crude polyphenolic extracts can be removed as described in Basic Protocol 2. The ethyl acetate used for elution of phenolic compounds other than anthocyanins is removed using a rotary evaporator at 20°C. The non-anthocyanin polyphenolics are dissolved in deionized distilled water and the pH is adjusted to 7.0 with NaOH as described in Alternate Protocol 2 or the method developed by Oszmianski and Lee (1990a). In the latter method, polyphenolics were fractionated into three groups neutral fraction A (flavanols and other polar phenolics), neutral fraction B (flavonols), and acidic phenolics. Polyphenolic extracts were adjusted to pH 7.0 with NaOH... [Pg.1247]

This fractionation step may be optional. Some samples can be directly analyzed by HPLC after filtration (step 2) without solid-phase extraction. Anthocyanins that can be detected at 280 nm can interfere with the separation of some polyphenolics. If the analyst is interested in nonanthocyanin polyphenolics, and especially if plant materials containing high levels of anthocyanins are being analyzed, this fractionation technique should be utilized. [Pg.1253]

The difference in wine making is also the reason for the differences in ochratoxin A concentration between white, rose, and red wine. White grapes are immediately pressed after harvest, whereas red wine grapes are mashed and macerated to extract anthocyanins from the berry skins. Maceration lasts either for several days at elevated temperature or for several hours using pectolytic enzymes after heating of the must to 80°C. [Pg.63]

Non-acidified methanol is the more suitable solvent to extract anthocyanins from grape reducing risks of hydrolysis of acetylated compounds 20 berry skins are extracted with 50 mL of methanol for 12h at room temperature (Revilla et al., 1998). Alternatively, the use of a methanol/water/formic acid 50 48.5 1.5 (v/v/v) mixture was reported as well (Gao et al., 1997). The volume of extract is reduced to about one-half under vacuum at 30 °C, adjusted to 100 mL with water, and 10 mL of this solution is diluted to 50 mL with water in order to further reduce the MeOH content. The resulting solution is purified by a SPE Ci8 cartridge (e.g., lg) previously activated by passage of... [Pg.193]

The difference between the M3G concentration in individual ferments (Table 1) could be partially attributed to the bin-to-bin variation of grapes having different maturity and might have been influenced by the choice of tank type and fermentation temperature (77). It remains to be established whether the peak M3G concentration reflected the maximum possible concentration of extractable anthocyanins under the conditions studied, or whether it was limited by the physical skin removal and could have been further increased by extended maceration on skins. [Pg.12]

Figure 1, Subcritical water extraction system for extracting anthocyanins from berry substrates. Figure 1, Subcritical water extraction system for extracting anthocyanins from berry substrates.
Acid violet 49 Acid yellow 3 Aluminum stearate Annatto (Bixa orellana) Annatto (Bixa orellana) extract Anthocyanins Beet (Beta vulgaris) extract ... [Pg.4984]

It is supposed, that packaged juice, as well as in the restored one and in juices of direct extraction, anthocyanin profile should be the same as the profile of fresh juice. [Pg.105]

The effeets of ethanol or mixtures of ethanol with hydroehlorie aeid (pH 4.1), citrie aeid (pH 4.9), tartaric acid (pH 5.0), lactic acid (pH 4.8) or phosphoric acid (pH 4.6) on the stability of extracted anthocyanins from blueberries have been investigated more detailed. It was observed that, apart the temperature and the pH-value, the type of acid influenced the extraction yield and the stability of the extracted anthocyanins [116,120]. [Pg.141]

The most common method for isolation of phenolic compounds found in fruits is the solvent extraction. Anthocyanins, like all polyphenols, are polar molecules thus polar solvents such as ethanol, methanol, water, acetone or mixtures of these are the most common solvents used in their extraction. The extraction can be conducted by grinding, drying, freeze-drying of fruits or only by soaking fresh fruits with subsequent solvent extraction [Kahkonen et al., 2001]. [Pg.163]


See other pages where Anthocyanins extraction is mentioned: [Pg.95]    [Pg.1246]    [Pg.38]    [Pg.31]    [Pg.36]    [Pg.156]    [Pg.94]    [Pg.336]    [Pg.473]    [Pg.248]    [Pg.494]    [Pg.223]    [Pg.252]   


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Anthocyanin extraction method

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Anthocyanins extracting from grape pomace

Anthocyanins extraction coefficient

Anthocyanins extraction during winemaking

Anthocyanins subcritical water extraction from

Column chromatography anthocyanin extraction

Ethanol anthocyanin extraction

Extraction of anthocyanins

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Extraction, Isolation, and Purification of Anthocyanins

Fractionation anthocyanin extraction

Solid-phase extraction anthocyanins

Use of Extracted Anthocyanin Derivatives in Nanostructures for Solar Energy Conversion

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