Embryonic fibroblasts

The biological impact of starch capped copper nanoparticles on mouse embryonic fibroblast (3T3L1) cells in vitro) was also evaluated by various parameters. More than 85 % of the 3T3Llcells were found to be viable, even after 20 hours time exposure which implies minimum impact on cell viability and morphology. The study demonstrates dose dependent cytotoxic potential of SCuNPs, that is non cytotoxic in the nanogram dose and moderately cytotoxic in the microgram doses (Fig. 10). Comparison of SCuNPs with Cu ions and uncapped copper nanoparticles (UCuNPs) revealed that, ions are more cytotoxic than SCuNPs. This observation supports the theory of slow release of ions from starch coated nanoparticles. 

Fig. 10. Effect of starch capped copper nanoparticles on cell viability (MTT assay) in case of mouse embryonic fibroblast (3T3L1).

Fig. 11. Photomicrographs of mouse embryonic fibroblast (3T3L1) cells (A) untreated, treated with (B) control (C) SOOng CuNPs, (D) lOOOng CuNPs.

It is well known that 1,10-phenanthrolines are highly active ironchelating agents. The parent compound itself has recently been shown to increase HIF-la levels in ocular tissue and to suppress 02-mediated epithelial cell proliferation when administered to mice [29]. A quantitative assay was developed to measure transcriptional potency of certain HIF stabilizers via an HRE-mediated (3-lactamase production in which the EC50 of 1,10-phenanthroline was measured to be approximately 8 pM. In addition, VEGF was dose-dependently produced in mouse embryonic fibroblasts by 1,10-phenanthroline with an EC50 of... 

Fig. 7.4 Normal human embryonic fibroblasts grown on fullerene on the surfaces (FoS) before (A) and after 30min illumination with halogen lamp 45mW/cm2 (B). Magnification l,200x (See Color Plates)...

Immortalized embryonic fibroblasts from PTEN-/- mice were shown to have elevated PtdIns(3,4,5)P3 level, constitutively elevated PKB/AKT activity and a decreased sensitivity to cell death in response to apoptotic stimuli (Stambolic et al, 1998). Homozygotic mutation of PTEN induced... 

In vitro studies with unmodified and modified N-terminal peptides of H3 demonstrated that Lys-14 acetylation did not interfere with methylation at Lys-9 by Suv39hl, while phosphorylation at Ser-10 and acetylation at Lys-9 did (Fig. 7). Further dimethylation of Lys-9 reduced enzymatic activity [186], A Suv39h double null primary mouse embryonic fibroblasts had higher levels of Ser-10 phos-phorylated H3 than wild type cells. These mutant cells had increased numbers of micro- and polynuclei. Oversized nuclei were characteristic of subpopulation of cells. The level of Lys-9 methylated FI3 in wild type cells and Suv39h double null cells was similar, demonstrating that other FI3 methyltransferases were involved [195]. Phosphorylation of Ser-10 by Ipll/aurora was also studied. Acetylation at Lys-14 promoted the activity of the mitotic kinase, while dimethylation, but not acetylation at Lys-9, reduced activity of the kinase [186]. 

Pfau, R., Tzatsos, A., Kampranis, S.C., Serebrennikova, O.B., Bear, S.E. and Tsichlis, P.N. (2008) Members of a family of JmjC domain-containing oncoproteins immortalize embryonic fibroblasts via a JmjC domain-dependent process. 

Irradiated wild type (WT) and CD 154 expressing (CD 154) NIH3T3 mouse embryonic fibroblasts (see Note 2) WT and CD154 NIH3T3 cells are cultured in complete DMEM and maintained in a humidified atmosphere of 5% CO at 37°C. 

Feeder cells G418 resistant murine embryonic fibroblasts. 

Jchibler Bert Van der Horst did an experiment where he showed that he couldn t induce circadian rhythms in Crj double knockout mouse embryonic fibroblasts. This is not surprising because they probably need the same components than in the clock. My strong prediction would be that everything is flat in the periphery. 

Kosbash It is fly behaviour. If we did Paolo Sassone-Corsi s experiment and took out the Drosophila embryonic fibroblasts, we could study individual cell function. 

Reconstitution of full-length BRCAl into mouse embryonic fibroblast cells with a disrupted BRCAl led to an increase in resistance to several DNA damaging agents, including the platinum compounds carboplatin and oxaliplatin, the topoisomerase 1 drugs irinotecan and topotecan, and the topoisomerase 11 drugs doxorubicin and etoposide (61). 

Oya, Y, Yamamoto, K. Tonomura, A. (1986) The biological activity of hydrogen peroxide I. Induction of chromosome-type aberrations susceptible to inhibition by scavengers of hydroxyl radicals in human embryonic fibroblasts. Mutat. Res., 172, 245-253... 

The culture of embryonic fibroblasts is used to obtain enough cells to perform prenatal diagnosis of inherited metabolic diseases (Box 1-D). Tissue culture is easiest with embryonic or cancer cells, but many other tissues can be propagated. However, the cells that grow best and which can be propogated indefinitely are not entirely normal the well-known HeLa strain of human cancer cells which was widely grown for many years throughout the world contains 70-80 chromosomes per cell compared with the normal 46. 

---