Mouse cells, culture

Hirt, 8. (1967) Selective extraction of polyoma DNA from infected mouse cell cultures. J. Mol. Biol. 119,487-506. 

Mycoplasma cells may fuse with the cells of eukaryotic hosts M. fermentans fused CD4 Molt, and CD4 12E lymphocytes in vitro without visible cytotoxicity [69]. Mycoplasma contamination of human tumor cell cultures is frequent with mycoplasma cells attached to tumor cells [70a, b, 71]. However, M. fermentans, or M. penetrans in embryonic mouse cell cultures activated the rc-related vav proto-oncogene, and induced malignant transformation src, Rous sarcoma vav, sixth letter of the Hebrew alphabet standing for linkage or connection) [72] recently reviewed in [73]. 

Siegel, B. V. Enhancement of interferon production by poly(rl) poly(rC) in mouse cell cultures by ascorbic acid. Nature (Lond.). 2M 1-, 197. ... 

Brinton, M. A., 1981, Isolation of a replication-efficient mutant of West Nile virus from a persistently infected genetically resistant mouse cell culture, J. Virol. 39 413. 

Historically, the development of animal cell culture systems has been dependent upon the development of new types of tissue culture media. Mouse L cells and HeLa cells were developed using a balanced salt solution supplemented with blood plasma, an embryonic tissue extract, and/or serum. In 1955 Eagle developed a nutritionally defined medium, containing all of the essential amino acids, vitamins, cofactors, carbohydrates, salts, and small amounts of dialyzed serum (Table 1). He demonstrated that this minimal essential medium (MEM) supported the long-term growth of mouse L and HeLa ceils. Eagle s MEM was so well defined that the omission of a single essential nutrient eventually resulted in the death of these animal cells in culture. 

Gao and Yamaguchi, 1999b Mouse bone marrow cells cultured for 7 d with bone resorbing factors (PTH, PGE2, EPS) +/- genistein osteoclast formation assessed by TRAP enzyme Genistein (10 Yi0 M) inhibited osteoclast formation. Mechanism may involve cAMP signalling. 

GAO Y H and YAMAGUCHi M (1999a) Inhibitory effect of genistein on osteoclast-like cell formation in mouse marrow cultures. Biochem Pharmacol 58, 767-72. 

May, L.A. et al.. Detection and quantitation of curcumin in mouse lung cell cultures by matrix-assisted laser desorption ionization time of flight mass spectrometry. Anal. Biochem., 337, 62, 2005. 

NoV are readily transferred from hands to fomites and vice versa (Bidawid et al., 2004 D Souza et al., 2006). The pronoimced environmental stability of NoV particles also contributes to the spread of outbreaks from point sources of surface contamination. All stability studies have made use of surrogate organisms to model NoV response to conditions, since the human virus is not easily grown in cell culture (Duizer et al., 2004b Straub et al., 2007). The murine norovirus (MNV) and the feline calicivirus (FCV) have both been used, with the mouse virus providing more... 

For other plant-derived antibodies, stability was shown to be similar to mammalian counterparts. For instance, a humanized anti-herpes simplex virus monoclonal antibody (IgGl) was expressed in soybean and showed stability in human semen and cervical mucus over 24 h similar to the antibody obtained from mammalian cell culture. In addition, the plant-derived and mammalian antibodies were tested in a standard neutralization assay with no apparent differences in their ability to neutralize HSV-2. As glycans may play a role in immune exclusion mechanisms in mucus, the diffusion of these monoclonal antibodies in human cerival mucus was tested. No differences were found in terms of the prevention of vaginal HSV-2 transmission in a mouse model, i.e. the plant-derived antibody provided efficient protection against a vaginal inoculum of HSV-2 [58]. This shows that glycosylation differences do not necessarily affect efficacy. 

Forgacs, Z., K. Paksky, P. Lazar, and E. Tatrai. 1998. Effect of Ni2+ on the testosterone production of mouse primary Leydig cell culture. Jour. Toxicol. Environ. Health 55A 213-224. 

Cytotoxicity has been evaluated against the cell culture KB of the human carcinoma of the nasopharynx and lymphocyte carcinoma of the mouse P388. Alkaloids tested are reported in Table XII. These data indicate that dimeric... 

The 5-HT4 receptor is a member of the seven transmembrane-spanning G protein-coupled family of receptors (GPCR) and constitutes an important subtype of the class of serotonin (5-HT) receptors. Initially, the 5-HT4 receptor was characterized in the neuronal cell culture [3] of mouse colliculi and was shown to be positively coupled to adenylyl cyclase. The effect of serotonin was mimicked by the 5-HT4 receptor agonists, BIMU 1 and BIMU 8, and was blocked by the 5-HT4... 

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