Balb/3T3 cells

Percival SS, Talcott ST, Chin ST, Mallak AC, Lound-Singleton A and Pettit-Moore J. 2006. Neoplastic transformation of BALB/3T3 cells and cell cycle of HL-60 cells are inhibited by mango (Mangifera indicaL.) juice and mango juice extract. J Nutr 136 1300-1304. 

Dunkel, V.C., R.J.Pienta, A.Sivak, and K.A.Traul. 1981. Comparative neoplastic transformation responses of Balb/3T3 cells, Syrian hamster embryo cells, and Rauscher murine leukemia virus-infected Fischer 344 rat embryo cells to chemical carcinogens. J. Natl. Cancer Inst. 67 1303-1315. 

Litton Bionetics. 1985. Evaluation of 2,5-dichlorophenol in the in vitro transformation of BALB/3T3 cells assay. Report to Bayer AG Institut Fuer Toxicology, West Germany, by Litton Bionetics, The Netherlands. 

Based on the above descriptions, we examined the inhibitory effect of the Artocarpus flavonoids on TNF-a release stimulated by okadaic acid using BALB/3T3 cells. This experiment was carried out in co-operation with Dr. Fujiki s group (Saitama Cancer Center Research Institute, Japan). All the compounds tested inhibit the TNF-a release stimulated by... 

Table 4. Inhibitory effects (1C , pmol/L) of six flavonoids for the release of TNF-a from BALB/3T3 cells by treatment of okadaic acid...

Atchison. M.. Chu, C.-S., Kakunaga, T. Van Duuren, B.L. (1982) Chemical cocarcinogenesis with the use of a subclone derived from BALB/3T3 cells with catechol as cocarcinogen. J. natl Cancer Inst., 69, 503-508... 

Barber ED, Cifone M, Rundell J, et al. 2000. Results of the L5178Y mouse lymphoma assay and the balb/3T3 cell in vitro transformation assay for eight phthalate esters. J Appl Toxicol 20 69-80. 

Polyamines (sper- BALB 3T3 cells Khan et al. (1990). Cell. Molec. Biology 36, 345-... 

Shibanuma, M., Koroki, T Nose, K. (1990). Stimulation by hydrogen peroxide of DNA synthesis, competence family gene expression and phosphorylation of a specific protein in quiescent Balb/3T3 cells. Oncogene 5,1025-1032. 

TNFa Inhibition of mouse TNF-a release from BALB/3T3 cells... 

E. Sabbioni, M. Fischbach, G. Pozzi, R. Pietra, M. Gallorini, J. L. Piette, Cellular retention, toxicity and carcinogenic potential of seafood arsenic. 1. Lack of cytotoxicity and transforming activity of arsenobetaine in the BALB/3T3 cell line, Carcinogenesis, 12 (1991), 1287-1291. 

In contrast to the constitutive release from tumour cells, hydrogen-peroxide release has been detected in Balb/3T3 cells [73], murine epidermal keratino-cytes[91] and rat pancreatic islets [92], but in each case release requires the stimulus of a phorbol ester. In the case of Balb/3T3 cells, platelet-derived growth factor (PDGF) was also stimulatory [73]. 

A positive response indicates a potential for maximum tolerated dose of less than 0.9 mmol/kg an LD50 value of less than 7.2 mmol/kg or a toxicity (IC50) for cultured BALB/3T3 cells of less than 1 ijM. 

Harada, A., Hanzawa, M., Saito, J. and Hashimoto, K. (1992). Quantitative Analysis of Structure-Toxicity Relationships of Substituted Anilines by Use of BALB/3T3 Cells. Environ. Toxi-col.C/iem.,J 2,973-980. 

Mouse C3H/10T1/2 clone 8 Mouse Balb/3T3 cells... 

Mouse Balb/3T3 cells Transformation No data ( + ) Dunkel et al. 1981... 

Crawford D, Zbinden I, Amstad P, Cerutti PA (1988) Oxidant stress induces the protooncogenes c-fos and c-myc in mouse epidermal cells. Oncogene 3 27-32 ShibanumaM, Kuroki T, Nose K (1988) Induction of DNA replication and expression of proto-oncogene c-myc and c-fos in quiescent Balb/3T3 cells by xanthine/xanthine oxidase. Oncogene 3 17-21... 

Mazzotti F, Sabbioni E, Ghiani M, Cocco B, Ceccatelli R and Foetanee S (2001) In vitro assessment of cytotoxicity and carcinogenic potential of chemicals evaluation of the cytotoxicity induced by 58 metal compounds in the Balb/3T3 cell line. Altern Lab Anim 29 601-611. 

Mazzotti F, Sabbioni E, Ponti J Ghiani M, Foetanee S and Rossi GL (2002) In vitro setting of dose-effect relationships of 32 metal compounds in the Balb/3T3 cell line, as a basis for predicting their carcinogenic potential. Altem Lab Anim 30 209-217. 

Balb 3T3 Cell transformation No data - Litton Bionetics 1985a... 

LBI. 1985a. Evaluation of 1C in the in vitro transformation of BALB/3T3 cells assay Final Report. Litton Bionetics, Inc. Chemical Manufacturers Association, Washington, DC. LBI Project No. 20992. 

MBOCA induced gene mutations at the thymidine kinase (TK) locus in mouse lymphoma cells (Caspary et al. 1988 Myhr and Caspary 1988). Unscheduled DNA synthesis (UDS) was induced in HeLa cells (Martin and Mcdermid 1981), in rat primary hepatocytes at >10 pmol (McQueen et al. 1981 Mori et al. 1988 Williams et al. 1982), and in hamster (McQueen et al. 1981) and rabbit (McQueen and Wiliams 1987) hepatocytes. The concentration that tested positive in the mouse was 50 jmol (McQueen et al. 1981). Sensitivity to MBOCA showed species-specific variations rat > mouse > hamster > rabbit (McQueen et al. 1981, 1983). Because hepatocytes have their own metabolic activation systems, no exogenous metabolic activation is needed. In assays using attachment independence as an end point, MBOCA, at concentrations near the LC o, transformed baby hamster kidney (Daniel and Dehnel 1981 Styles 1981), rat embryo (Dunkel et al. 1981 Traul et al. 1981), and Balb/3T3 cells (Dunkel et al. 1981). Transformation assays have not been evaluated... 

Sakai, A. and Fujiki, H., Promotion of BALB/3T3 cell transformation by the okadaic acid class of tumor promoters, okadaic acid and dinophysistoxin-1, Jpn. J. Cancer Res., 82, 518, 1991. 

Komori, A. et al., Tumor necrosis factor acts as a tumor promoter in BALB/3T3 cell transformation. Cancer Res., 53, 1982, 1993. 

The procedures for testing the biological activity of the polymers have also been previously described (126,127). Balb/3T3 cells, either normal or transformed by the Moloney murine sarcoma... 

The biological activity of the platinum polyamines was tested in cell culture using two cell lines derived from mouse fibroblasts. Balb/3T3 cells resemble normal cells in their growth properties m-MSV Balb/3T3 cells have been transformed and resemble cancer cells in their growth properties. 

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