Primary hepatocytes

Hepatocyte primary cultures Hepatocyte primary cultures Cell transformation Syrian hamster embryo cells... 

Williams GM, Tong C, Brat SV. 1985. Tests with the rat hepatocyte primary culture/DNA-repair test. In Ashby J, de Serres FJ, et al., eds. Progress in mutation research. Vol. 5. Evaluation of short-term tests for carcinogens. Amsterdam, The Netherlands Elsevier Science Publishers,... 

Parzefall W, ErberE, Sedivy R et al (1991) Testing for induction of DNA synthesis in human hepatocyte primary cultures by rat liver tumor promoters. Cancer Res 51 1143-1147... 

Maslansky, C.J. and Williams, G.M. (1985). Methods for the initiation and use of hepatocyte primary cultures from various rodent species to detect metabolic activation of carcinogens. In In vitro Models for Cancer Research (Webber, M. and Sekely, L., Eds.). CRC Press, Boca Raton, FL, pp. 43-60. 

McQueen, C.A. and Williams, G.M. (1981). Characterization of DNA repair elicited by carcinogens and drugs in the hepatocyte primary culture/DNA repair test. J. Toxicol. Environ. Health 8 463-477. 

Murayama J-I, Ishiwata M, Fukui M, et al. 1990. Comparative acute cytotoxicities of 37 xenobiotics detected in drinking water to rat hepatocyte primary culture. Eisei Kagaku 36(4) 267-276. 

Williams GM, Laspia MF, Dunkel VC. 1982. Reliability of the hepatocyte primary culture/DNA repair test in testing of coded carcinogens and noncarcinogens. Mutat Res 97 359-370. 

Long RM, Moore L. 1988. Biochemical evaluation of rat hepatocyte primary cultures as a model for carbon tetrachloride in rats based on arterial blood inhaled air concentration ratios. Toxicol AppI Pharmacol 92 295-306. 

Kombrast, D.J. Barfknecht, TR. (1984) Comparison of 7 azo dyes and their azo rednction products in the rat and hamster hepatocyte primary crrltrrre/DNA-repair assays. Mutat. Res., 136, 255-266... 

Fujioka, T. Ui, M. Involvement of insulin receptor substrates in epidermal growth factor induced activation of phosphatidylinositol 3-kinase in rat hepatocyte primary culture. Eur. J. Biochem., 268, 25-34 (2001)... 

Williams, G.M., Tong, C. Ved Brat, S. (1985) Tests with the rat hepatocyte primary culture/ DNA repair test. Prog. Mutat. Res., 5, 341-345... 

McQueen, C.A., Kreiser, D.M. Williams, GM. (1983) The hepatocyte primary culture/DNA repair assay using mouse or hamster hepatocytes. Environ. Mutag., 5, 1-8... 

WHO (1993) Guidelines for Drinking Water Quality, 2nd Ed., Vol. 1, Recommendations. Geneva Williams, G.M., Laspia, M.F. Dunkel. VC. (1982) Reliability of the hepatocyte primary culture/DNA repair test in testing of coded carcinogens and noncarcinogens. Mutat. Res., 97,... 

Mori, H., Sugie, S., Yoshimi, N., Iwata, H.,Nishikawa, A., Matsukubo, K.. Shimizu. H. Hirono, I. (1988) Genotoxicity of a variety of hydrazine derivatives in the hepatocyte primary culture/ DNA repair test using rat and mouse hepatocytes. Jpn. J. Cancer Res., I ). 204-211... 

Martelli A, Robbiano L, Giuliano L, et al. 1985. DNA fragmentation by N-nitrosodimethylamine and methyl methanesulfonate in human hepatocyte primary cultures. Mutat Res 144 209-211. 

A15. Andus, T., Geiger, T., Hirano, T., Kishimoto, T., Tran Thi, T. A., Decker, K., and Heinrich, P. C. Regulation of synthesis and secretion of major rat acute-phase proteins by recombinant human interleukin-6 (BSF-2/IL-6) in hepatocyte primary cultures. Eur. J. Biochem. 173, 287-293 (1988). 

San, R. H. C. and Williams, G. M., Rat hepatocyte primary cell culture-mediated mutagenesis of adult rat liver epithelial cells by procarcinogens. Proc. Soc. Exp. Biol. Med. 156 534-538 (1977). 

By using the hepatocyte primary culture DNA repair test [247] with liver cells of adult ACI rats, an essay that is capable to discern true carcinogens from solely mutagenic compounds such as quercetin and other bioactive flavonoids, Japanese workers [248] were able to show that ptaquiloside is a genotoxic carcinogen. The key evidence was that ptaquiloside induced DNA repair in the exposed liver cells, which could be interpreted as an intrusion into the DNA molecule that caused enough distortions to trigger the reconstruction mechanism. 

Wang H H, Lautt W W (1997). Hepatocyte primary culture bioassay a simple tool to assess the initiation of the liver regeneration cascade. J. Pharmacol. Toxicol. Meth. 38 141-150. 

McQueen CA, Williams GM. 1987. The hepatocyte primary culture/DNA repair test using hepatocytes from several species. Cell Biology and Toxicology 3(2) 209-218. 

Mori H, Yoshimi N, Sugie S, et al. 1988. Genotoxicity of epoxy resin hardeners in the hepatocyte primary culture/DNA repair test. Mutat Res 204(4) 683-688. 

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