Ames assay

Benzyl chloride induced a positive mutagenic response ia the Ames Assay ia strain TA 100 with and without rat Hver S-9 metaboHc activation. 

Although trichloroethylene itself may not be genotoxic, several of its metabolites are reactive and potentially genotoxic compounds (Miller and Guengerich 1982). Several isomers of 1,2-dichlorovinyl-cysteine, a product of trichloroethylene metabolism in the kidney, are mutagenic in the in vitro Ames assay... 

Salmeen IT, AM Pero, R Zator, D Schuetzle, TL Riley (1984) Ames assay chromatograms and the identification of mutagens in diesel particle extracts. Environ Sci Technol 18 375-382. 

The mutagenic potential of diisopropyl methylphosphonate was investigated using the Ames assay. The compound was obtained from two different sources and tested on Salmonella typhimurium strains TA-1535, TA-1537, TA-1538, TA-98, and TA-100, both with and without S-9 activation. The compound did not demonstrate mutagenic activity in any of the assays (Hart 1980). Diisopropyl methylphosphonate was also negative for gene mutation in Saccharomyces cerevisiae (Hart 1980). 

Prokaryotic organisms Salmonella typhimurium (Ames assay) Gene mutation — FMC 1992b Durad 550B... 

Fluoranthene is one of the more prevalent PAH in the human environment. Although fluoranthene is not active as a carcinogen, its 2- and 3-methyl derivatives have been shown to be active as tumor initiators (105). The major mutagenic metabolite of fluoranthene in the Ames assay has been identified as the 2,3-dihydrodiol (31)... 

Benzo(b)-, benzo(k)-, and benzo(j)fluoranthene are common environmental contaminants (38). The tumor-initiating activity of benzo-(b)fluoranthene on mouse skin is about equal to that of DBA (38). All three isomers are mutagenic in the Ames assay (110). Syntheses of the 1,2-, 9,10-, and 11,12-dihydrodiols of benzo(b)fluoranthene by Method I have been reported (111,112). 

BL and Ames assays for screening of contaminated sediment a comparison... 

Hughes TJ, Simmons DM, Monteith LG, et al. 1987. Mutagenicity of 31 organic compounds in a modified preincubation Ames assay with Salmonella typhimurium strains TA100 and TA102. Environ Mutagen 9 49. 

S typhi muri urn (modified Ames assay) Gene mutation... 

Brusick D. 1983. Evaluation of chronic rodent bioassays and Ames assay tests as accurate models for predicting human carcinogens. In Milman HA, ed. Application of biological markers for carcinogen testing, 153-163. 

Blackburn GR, Deitch RA, Schreiner CA, et al. 1984. Estimation of the dermal carcinogenic activity of petroleum fractions using a modified Ames assay. Cell Biol Toxicol 1 67-80. 

Blackburn G, Deitch R, Schreiner C, et al. 1987. Testing of petroleum middle distillates in a modified Ames assay. Environmental Mutagenesis 9 15. 

Ames, B. N, and Hooper, K Does carcinogenic potency correlate with mutagenic potency in the Ames assay Nature (1978) 274, 19. 

Charles JM, Cunny HC, Wilson RD, et al Ames assays and unscheduled DNA synthesis assays on 2,4-dichlorophenoxyacetic acid and its derivatives. Mutat Res 444 207-16, 1999... 

Hydrogen sulfide was not mutagenic in Ames assays with or without metabolic activation. ... 

Titton Bionetics. 1980a. Sister chromatid exchange assay, Ames assay, mouse lymphoma forward mutation assay, and transformation assay for a sample containing 33-1/3% each ortho-, meta-, and para-cresol. Unpublished data submitted to EPA/OTS. Fiche no. OTS0517528. 

---