Stress-Induced Hyperthermia

This difference m body temperature is called stress-induced hyperthermia (SIH) and is thought to reflect an anticipatory type of anxiety. Following pretreatment of all mice hvmg m the same cage with an anxiolytic compound (chlordiazepoxide m the example given m Fig. 10), the body temperature of the... 

I hour after treatment the rectal temperature is determined and mice are consecutively placed in another cage Stress-induced hyperthermia defined as the della of the median rectal temperature within the six initially removed mice and Ihe median of the six last removed mice within a cage... 

Spooren, W.P.J.M., Schoeffter, P., Gasparini, F., et al. Pharmacological and endocrinological charaterisation of stress-induced hyperthermia in singly housed mice using classical candidate anxiolytics CLY314582. MPEP and NKP608). Eur. J. Pharmacol. 435, 161-170, 2002. 

Chinese angelica Angelica sinensis) is the most important female tonic remedy in Chinese medicine. The elfects of angelica EO in three assays in mice (elevated plus maze, light/dark and stress-induced hyperthermia test) suggested that angelica EO exhibited an anxiolytic-like effect [391]. A link to emotion and cognitive performance with the olfactory system was reported [392]. Moreover, the EOs could affect mood, concentration and sleep [393], while other studies had shown that EOs were potentially important to boost the immune system [394, 395]. 

Bouwknecht, J. A., Olivier, B. and Paylor, R. E. (2007) The stress-induced hyperthermia paradigm as a physiological animal model for anxiety a review of pharmacological and genetic studies in the mouse. Neurosci Biobe-hav Rev 31, 41-59. 

Li, X., Morrow, D. and Witkin, J. M. (2006) Decreases in nestle shredding of mice by serotonin uptake inhibitors comparison with marble burying. Life Sci 78, 1933-1939. Olivier, B., Zethof, T., Pattij, T., van Boogaert, M., van Oorschot, R., et al. (2003) Stress-induced hyperthermia and anxiety pharmacological validation. Eur J Pharmacol 463, 117-132. 

Stress-induced hyperthermia Body temperature response v=> Plasma corticosterone <=> Body temperature response to 5-HT1A agonist 66... 

Iijima, M., Shimazaki, T., Ito, A. and Chaki, S. (2007) Effects of metabotropic glutamate 2/3 receptor antagonists in the stress-induced hyperthermia test in singly housed mice. Psychopharmacology, 190, 233-239. 

Rose and lavender oil, administrated intraperitoneally (ip) to mice, showed anticon ict effects (Umezu 1999 Umezu et al. 2002), suggesting anxiolytic properties. Unequivocal anxiolytic properties were also demonstrated for lavender EO (ip, gerbils) with the elevated plus maze test (Bradley et al. 2007). Chen et al. (2004) reported Xh i Angelica sinensis (Oliv.) Diels (Apiaceae) EO (orally administrated, mice) also has an anxiolytic effect in the elevated plus maze, the light/dark model, and the stress-induced hyperthermia paradigms. 

While most strains respond consistently to this paradigm due to its independence from motor activity, specific mouse strains (e.g., FVB/N) have considerably higher baseline body temperatures and should be avoided in this model (21). However, this procedure has been shown to effectively induce hyperthermia to varying degrees in all inbred and outbred strains tested (41) and is also an effective indicator of stress in genetically modified animals (34). 

Burgman PW, Kampinga HH, Konings AW (1993) Possible role of localized protein denaturation in the mechanism of induction of thermotolerance by heat, sodium-arsenite and ethanol. Int J Hyperthermia 9 151-162 Cajone F, Bernelli-Zazzera A (1988) Oxidative stress induces a subset of heat shock proteins in rat hepatocytes and MHICI cells. Chem Biol Interact 65 235-246 Caltabiano MM, Koestler TP, Poste G, Greig RG (1986a) Induction of 32- and 34-kDa stress proteins by sodium arsenite, heavy metals, and thiol-reactive agents. J Biol Chem 261 13381-13386... 

Data indicating participation of PolyPs in overcoming stress were obtained in Propi-onibacteria. The PolyP component in the 31P NMR spectra of Propionibacterium acne increased after ultraviolet light irrardiation (Kjeldstad and Johnson, 1987) and after hyperthermia treatment (Kjeldstad et al., 1988). Such treatments, carried out in triplicate, induced an increase in the PolyP content, as observed by 31P NMR spectroscopy. One of the explanations for this might be that hyperthermia and ultraviolet light induce an oxidative stress in the cells, which increases the amount of PolyP (Kjeldstad and Johnson, 1987 Kjeldstad et al., 1988). 

Johnson Rowsey, P., Yang, Y. L., and Gordon. C. J. (2002). Peripheral cholinergic pathway modulates hyperthermia induced by stress in rats exposed to open-field. stress. J. Appl. Phy.siol. 92, 789-794. 

Sodium arsenite and hyperthermia both induce a similar stress response in rat embryos treated in vitro as measured by stress protein synthesis and accumulation of hsp70 mRNA and hsp72 expression, although the abnormal morphologies produced by these embryotoxic agents appear to be different (Mirkes and Cornel 1992). Studies conducted by Amaral et al. (1988) have shown that arsenite does not inhibit synthesis of constitutive, nonstress proteins as dramatically as does hyperthermic shock. Several studies have... 

The intracellular localization of stress proteins is problematic for the evaluation of the response in humans. Because the cells for the assay of stress proteins are not readily available through noninvasive procedures, the application of this response to human monitoring is limited. Recently, however, enhanced synthesis of stress proteins was demonstrated in primary cultures of human lymphocytes exposed to several metals (Yamada and Koizumi 1993). The specificity of the response was dependent on the metal to which the cultures were exposed. For example, cadmium and zinc induced both hsp70 and MT, while cobalt and triphenyltin induced only hsp70. Conversely, copper, mercury, nickel, and silver all induced synthesis of MT, but not of hsp70. Enhanced synthesis of stress proteins has also been demonstrated in vivo in lymphocytes and spleen cells excised from mice exposed to hyperthermia (Rodenhiser et al. 1985). 

Mirkes PE (1987) Hyperthermia-induced heat shock response and thermotolerance in postimplantation rat embryos. Dev Biol 119 115-122 Mirkes PE, Cornel L (1992) A comparison of sodium arsenite- and hyperthermia-induced stress responses and abnormal development in cultured postimplantation rat embryos. Teratology 46 251-259... 

Hyperthermia. - The ability to tolerate heat stress decreases with age. EPR studies on frozen liver samples from rats have revealed changes in the intensity of signals from endogenous metals and a semiquinone species. Related studies have shown that hyperthermia induces the appearance of signals from ceruloplasmin, haem-coordinated NO and a semiquinone species in the blood. These changes were attributed to increased NOS and XO activities and were proposed to underlie the observed systemic responses e.g. hypotension). Other workers have examined the mechanisms by which radicals enhance the induction of apoptosis in human lymphoma cells exposed to hyperthermia. ... 

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