Responder strains

Fu, Y. and Blankenhom, E.R, Nitric oxide-induced anti-mitogenic effects in high and low responder strains, J. Immunol., 148, 2217, 1992. 

Initially we Investigated whether Immunization of H-2< and H-2S mice (two high responder strains to Mb refs. 46,47) with free synthetic peptides of Increasing length and carryTng site 5 (Figure 9) could evoke antibodies that will react with native Mb (48). Thus Immunization with peptides of 22 amino acids (peptide 137-153), 11 amino acids (peptide 143-153), 9 amino acids (peptide 145-153) or 7 amino acids (peptide 145-151), each of which... 

Diaz-Sanchez, D. and Kemeny, D.M. (1991). Generation of a long-lived IgE response in high and low responder strains of rat by co-administration of ricin and antigen. Immunology 72, 297-303. 

Jiang, Y. and G. Moller. In vitro effects of HgCl2 on murine lymphocytes. I. Preferable activation of CD4+ T cells in a responder strain. J. Immunol. 154 3138-3146, 1995. 

There were, however, some indications that in some systems two H-linked Ir genes might be required for responsiveness. Rare instances had been reported where Fj hybrids between two low responder strains gave responses higher than either parental strain In addition, Stimpfling and Durham also obtained evidence that responses to the histocompatibility specificity H-2.2 might be controlled by two genes. 

Several F hybrids were then immunized with GL0 (see Table 4.5). All of the hybrids made between responder and non-responder strains were responders to this antigen, indicating the expected dominance of the Ir-GLcj) gene. More interestingly, however, the (A x B10)Fi x 18R)Fi hybrids,... 

Where do each of the two genes operate at the cellular level The recent reports by Taussig et alP and Mozes et alP (discussed in Section 4.7), indicating that the genetic defect in non-responder strains to (T,G)-A-L is found in B cells in some strains and in both T and B cells in other strains might be ex-... 

In vitro cultures of spleen cells from inbred and congenic-resistant strains in the presence of 1 or 10 lug of soluble GAT have shown identical results as those observed in vivo. Soluble GAT in these concentrations stimulates the development of IgG GAT-specific PFC responses in cultures of spleen cells from responder C57BL/6 (H-2 ), Fi(C57 x SJL) (H-2 ) and A/J (H-2") mice. Soluble GAT did not stimulate the development of GAT-specific PFC responses in cultures of spleen cells from non-responder H-2 mice (SJL, BIO.S, A.SW) or H-2 mice (DBA/1). It is of interest to note that GAT did not stimulate antibody production of the IgM class by responder or non-responder strains either in vivo or in vitro. The GAT terpolymer differs, therefore, markedly in this respect from the branched polymers such as (T,G)-A--L which stimulate the synthesis of IgM antibodies in both responder and nonresponder strains. 

The genetic differences between responder and non-responder mouse strains in the response to (T,G)-A- -L (i.e. the production of IgG antibodies and the development of specific memory by the responder strains) are abolished by... 

The demonstration that non-specific T cell stimulation of B cells by the allogeneic effect can stimulate strong antibody responses by genetic nonresponder strains and the finding that tetraparental mice generated from responder and non-responder strains behave as responders to (T,G)-A- -L and synthesize antibody of non-responder allotype may be considered further evidence for the lack of expression of H-linked Ir gene function in B cells in this system. 

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