Staining methods

Stacey also initiated a study of the widely used Dische and Feulgen staining methods. When he commenced his investigations, knowledge of the chemical basis of the tests was limited, although there was extensive literature on applications of the Feulgen reaction. 

Cell count staining method with Trypan Blue (see e.g. [68])... 

A dual isotope labeling technique [85] has been used to measure membrane permeability in plant cells, based on the selective permeabiHty of the membranes of living cells to tritiated water and carbon-14 labeled mannitol. Kieran [29] showed that the results of the dual isotope labeling and Evan s Blue staining methods correlated well as indicators of cell viability however, the latter was preferable in terms of reagent cost and ease of analysis. 

Genetic engineering of PHA synthase proteins in E. coli in combination with a suitable viable-colony staining method for the screening of PHA content [143] will make many methodological tools applicable to PHA biosynthesis genes and proteins and will allow studies on structure to function relation-... 

Leong3 postulated that internal controls were required to optimize the variable influences resulting from aspects of tissue preparation and factors intrinsic to the staining method. Various controls have been employed, includ-... 

Zeheb R. Automating immunohistochemistry. In Immunohistochemical Staining Methods, 4th edition, ed. ME Key, pp. 103-106. Carpinteria, CA Dako, 2006. 

Van der Loos CM. Immunoenzyme Multiple Staining Methods. Oxford, UK Bios Scientific Publishers, 1999. 

Non-gynecologic directly smeared brushing is prepared, stained by Papanicolaou staining method and coverslipped for microscopic examination. 

The needle rinse or the fluid retrieved from the FNA is prepared following the cytocentrifugation procedure and stained with Papanicolaou staining method. 

De Mey, J., Moeremans, M., Ceuens, G., Nuydens, R., and De Brabander, M. (1981) High resolution light and electron microscopic localization of tubulin with the IgS (immuno gold staining) method. Cell Biol. Int. Rep. 5, 889. 

De Waele, M., Renmans, W., Segers, E., De Valck, V., Jochmans, K., and van Camp, B. (1989) An immunogold-silver staining method for detection of cell surface antigens in cell smears. J. Histochem. Cytochem. 37, 1855-1862. 

Duan, L., Wang, Y., Li, S.S-C., Wan, Z., and Zhai, J. (2005) Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano-gold immunological amplification and silver staining method. BMC Infect. Dis. 5, 53. http //www.biomedcentral. com/1471-2334/5/53. 

Eleven plant species (Table 3) were preliminarly tested for their response to the Feulgen staining method (described below), which is essential for the efficient microscope observation of genetic anomalies of cells. This, in order to select plants to be successively used in the experiments with the mutagenic compounds. 

Suberized lamellae in casparian strips can be located using the berbe-rine-aniline blue fluorescence staining method, which works equally... 

Introduction The Primary Antibodies Visualization of the Target/Staining Methods Enhancing of Signals with Antibody Complexes A Protocol for Immunovisualization of Proteins with Alkaline Phosphatase Conclusions References... 

Crissman HA, Oka MS, Steinkamp JA. Rapid staining methods for analysis of deoxyribonucleic acid and protein in mammalian cells. J Histochem Cytochem 1976 24 64-71. 

Lewis PR. Cytochemical Staining Methods for Electron Microscopy, Elsevier, Amsterdam, The Netherlands, 1992. 

Finally, the reader is referred to Van Nooden and Hulstaert (46) and Lewis and Knight (47) for reviews of cytochemical staining methods for electron microscopy. Continual improvements in ultrastructural cytochemistry are anticipated. 

Knight DR, Lewis PR. General cytochemical methods, in Cytochemical Staining Methods for Electron Microscopy. Practical Methods in Electron Microscopy (Glauert A, ed.), Elsevier, Amsterdam, 1992, pp. 79-145. 

By means of intracellular recording and staining methods, we have examined the responses of AL neurons to stimulation of the ipsilateral antenna with each of the sex pheromone components as well as partial and complete blends (75). In accordance with results of behavioral and sensory-receptor studies, components A and B are the most effective and potent sex pheromone components for eliciting physiological responses in the male-specific AL neurons. On the basis of these responses, we classified the neurons into two broad categories pheromone generalists and pheromone specialists (76). Pheromone generalists are neurons that respond similarly to stimulation of either the component A input channel or the component B input channel and do not respond differently when the complete, natural pheromone blend is presented to the antenna. In contrast, pheromone specialists are neurons that can discriminate between antennal stimulation with component A and stimulation with component B. There are several types of pheromone specialists. Some... 

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