Immunogold silver staining

De Waele, M., Renmans, W., Segers, E., De Valck, V., Jochmans, K., and van Camp, B. (1989) An immunogold-silver staining method for detection of cell surface antigens in cell smears. J. Histochem. Cytochem. 37, 1855-1862. 

Ellis, I.O., Bell, J., and Bancroft, J.D. (1988) An investigation of optimal gold particle size for immuno-histological immunogold and immunogold-silver staining to be viewed by polarized incident light (EPI polarization) microscopy./. Histochem. Cytochem. 36, 121-124. 

Gillitzer, R., Berger, R., and Moll, H. (1990) A reliable method for simultaneous demonstration of two antigens using a novel combination of immunogold-silver staining and immunoenzymatic labeling. J. Histochem. Cytochem. 38, 307-313. 

Holgate, C., Jackson, P., Cowen, P., and Bird, C. (1983) Immunogold-silver staining new method of immunostaining with enhanced sensitivity./. Histochem. Cytochem. 31, 938. 

Cassab GI, Varner JE. Immunocytolocalization of extensin in developing soybean seed coats by immunogold-silver staining and by tissue printing on nitrocellulose paper. J Cell Biol 1987 105 2581-2588. 

APES may interfere with silver salt solutions in intensification steps of immunogold silver-staining techniques therefore, for those applications, poly-L-lysine or gelatin-coated slides are preferable. 

De Waele, M., De Mey, J., Rewmans, W, Labeur, C., Rochmans, K., and Van Kamp, B. (1986) Potential of immunogold-silver staining for the study of leukocyte sub-populations as defined by monoclonal antibodies. J Histochem. Cytochem. 34, 1257—1263. 

Gao, K, GifFin, B F, Morris, R E, Cardell, E L, and Cardell, R. R (1994) Optimal visualization of immunogold-silver staining of hepatic PEPCK with epipolarized light microscopy J Histochem Cytochem 42, 823-826. 

Otsuki, Y., Maxwell, L. E, Magan, S., and Kubo, H. (1990) Immunogold-silver staining method for light and electron microscopic detection of lymphocyte cell-surface antigens with monoclonal antibodies. J Histochem Cytochem. 38, 1215—1221. 

Lackie, P M.Hennessy, R J, Hacker, G W, and Polak, J M (1985) Investigation of immunogold-silver staining using electron microscopy Histochemistry 83, 545-550... 

Manara, G C, Ferrari, C, Torresani, C., Sansoni, P, and De Panfilis, G. (1990) The immunogold-silver staining approach in the study of lymphocyte subpopula-tions in transmission electron microscopy. J Immunol Methods 128, 59-63... 

Moeremans, M., Daneels, G, van Dijck, A, Langanger, G., and De Mey, J. (1984) Sensitive visualization of antigen-antibody reactions in dot and blot immune overlay assays with immunogold and immunogold/silver staining. J Immunol Methods 74, 353-360. 

Jackson et al. (1988) were the first to employ the immunogold-silver staining (IGSS) method in combination with microwave heating. They completed within minutes the incubations in primary and secondary antibodies for detecting immunoglobulins in paraffin sections of human tonsil, van de Kant et al. (1990) applied the same method, except that resin instead of paraffin sections were used to detect bromodeoxyuridine incorporated in cells of the mouse testis. Tissue morphology is preserved better in a resin than in paraffin. The former also allows the use of thinner sections. 

Hayat, M. A. (ed.) 1995. Immunogold-Silver Staining Principles, Methods, and Applications. CRC Press, Boca Raton. FL. 

Jackson, P., Lalani, E. N., and Boutsen, J. 1988. Microwave-stimulated immunogold silver staining. Histochem. J. 20 353-358. 

Taban, C. H., and Cathieni, M. M. 1995. Microwave-aided binding of colloidal gold-protein-sub-stance P. In Immunogold-Silver Staining Principles, Methods, and Applications (M. A. Hayat, ed.), pp. 183-195. CRC Press, Boca Raton, FL. 

Wang, B. L., Springer, G. F., and Harwick, L. C. 1998. T (Thomsen-Friedenreich) and Tn epitope location and their spatial relations to adhesion plaques on human breast carcinoma cells Immunogold silver staining studies at scanning electron microscopic level. Submicrosc. Cytol. Pathol. 30 503-509. 

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