Analytical methods fluorescent staining

With CE, separated analytes are detected and measured as they migrate past an optical detector without prior staining. Optical detection is based on classical methods, such as photometric absorbance, refractive index, and fluorescence (see Chapter 3). These techniques miniaturize and simplify the process of measurement by eliminating several of the steps required in conventional gel electrophoresis. Because the amount of protein measured and the measurement path are both very small, however, special conditions are often used to improve sensitivity. 

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